
JonathanT
168 strain (parent strain of this repo)
This is the initial strain in this repository obtained from the shown reference. The attached file describes the transformation protocol used.
This strain was barcoded with barcode BC659 following the Cre-Lox protocol in between the ybxH and ybxI genes (zeocin resistance removed).
The genome of this strain is attached.
Burkholder, P. R., & Giles, N. H. (1947). INDUCED BIOCHEMICAL MUTATIONS IN BACILLUS SUBTILIS. American Journal of Botany, 34(6), 345–348. doi:10.1002/j.1537-2197.1947.tb12999.x
Strain data
Bacillus subtilis
ATCC 6051168 (BC659)
The initial aliquot of 168 cells was obtained from our strain collection in Devonshire Building.
trpC2
Barcode: BC659
Trp(-)
Devonshire Building. ICOS -80 room. Freezer 2. Box 2.
May 16, 2019