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JonathanT

Last updated: Jun 06, 2020

BW25113 - BC383 (CRISPR)

This is the barcoded version of BW25113 using CRISPR as barcoding method (Li et al. 2015). The attached plasmid contains the gRNA and the repair template.

Draft
Digital Barcode (Version 1.0)

8328b553bfd349f098d19ce9d95e05d6b2c0ad6eb

Physical Barcode (Version 1.0)
GTCGTGTCTGTCAATATCGGGGATAATAAATGTAGATAATGATTTCGTCAAGTCCTTACCCCGACTATACAACAGCAATCCATAGTAATCTGTGCGAATGGTTTAGTAGGACATAACACAAAT

Li Y, Lin Z, Huang C, et al. Metabolic engineering of Escherichia coli using CRISPR-Cas9 meditated genome editing. Metab Eng. 2015;31:13‐21. doi:10.1016/j.ymben.2015.06.006

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Strain data

Escherichia coli

ATCC 11775

BW25113 - BC (CRISPR)

lacI+ rrnBT14 ΔlacZWJ16 hsdR514 ΔaraBADAH33 ΔrhaBADLD78
BC383

Devonshire Building. ICOS -80 room. Freezer 2. Box 1.

Aug 07, 2019