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JonathanT

Last updated: Jun 06, 2020

BW25113 - BC383 (CRISPR)

This is the barcoded version of BW25113 using CRISPR as barcoding method (Li et al. 2015). The attached plasmid contains the gRNA and the repair template.

Draft
Whole genome sequence

Li Y, Lin Z, Huang C, et al. Metabolic engineering of Escherichia coli using CRISPR-Cas9 meditated genome editing. Metab Eng. 2015;31:13‐21. doi:10.1016/j.ymben.2015.06.006

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Strain data
Barcode
v1 8328b5

8328b553bfd349f098d19ce9d95e05d6b2c0ad6eb

v1 GTCGTG

GTCGTGTCTGTCAATATCGGGGATAATAAATGTAGATAATGATTTCGTCAAGTCCTTACCCCGACTATACAACAGCAATCCATAGTAATCTGTGCGAATGGTTTAGTAGGACATAACACAAAT

Escherichia coli

ATCC 11775

BW25113 - BC (CRISPR)

lacI+ rrnBT14 ΔlacZWJ16 hsdR514 ΔaraBADAH33 ΔrhaBADLD78
BC383

Devonshire Building. ICOS -80 room. Freezer 2. Box 1.

Aug 07, 2019