AU

Nadia Rostami

Last updated: Oct 08, 2024

pKTM25

Bacterial two-hybrid vector designed to express a given polypeptide fused in frame at its N-terminal end with TM domain 1 of E. coli oppB and T25 fragment, p15 ori, KmR The TM region was PCR amplified from pSTM25b with primer pair 1/2 cut with SalI/EcoRI and cloned into SalI/EcoRI digested pKT25

This plasmid was propagated using 10-beta strain: Δ(ara-leu) 7697 araD139 fhuA ΔlacX74 galK16 galE15 e14-  ϕ80dlacZΔM15  recA1 relA1 endA1 nupG  rpsL 

Primers 1 and 2

1. CCGACAACGACGTCAACAG

2. CCAGGGTTTTCCCAGTCA

 

Commited
Whole genome sequence

Koch B, Callaghan MM, Tellechea-Luzardo J, Seeger AY, Dillard JP, Krasnogor N. Protein interactions within and between two F-type type IV secretion systems. Mol Microbiol. 2020;114(5):823-838. doi:10.1111/mmi.14582

Strain data
Barcode
v0 19d9d9

19d9d97cde74ed98ea023c1163ff5356b2c359335

plasmid

ATCC plasmid

pKTM25

Birgit Koch created this plasmid from the parent plasmid pKT25

-80 freezer- Birgit Koch collection- Devonshire building