pKTM25

Bacterial two-hybrid vector designed to express a given polypeptide fused in frame at its N-terminal end with TM domain 1 of E. coli oppB and T25 fragment, p15 ori, KmR The TM region was PCR amplified from pSTM25b with primer pair 1/2 cut with SalI/EcoRI and cloned into SalI/EcoRI digested pKT25

This plasmid was propagated using 10-beta strain: Δ(ara-leu) 7697 araD139 fhuA ΔlacX74 galK16 galE15 e14-  ϕ80dlacZΔM15  recA1 relA1 endA1 nupG  rpsL 

Primers 1 and 2

1. CCGACAACGACGTCAACAG

2. CCAGGGTTTTCCCAGTCA