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JonathanT

Last updated: Jul 16, 2020

pKT25TraVF

TraVF was PCR amplified using primer pair 59/60  and cloned into pKT25.

Draft
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v1 ac64f7

ac64f78ea09c4a20a34951c2c2616289b2cf18df1

Whole genome sequence

This plasmid was constructed in this work: Protein interactions within and between two F-type type IV secretion systems. Birgit Koch, Melanie M. Callaghan, Jonathan Tellechea Luzardo, Ami Y. Seeger, Joseph P. Dillard, and Natalio Krasnogor. 2020. Submitted to Molecular Microbiology. The backbone (pKT25) comes from: Euromedex

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Strain data

Plasmids

This plasmid was propagated using 10-beta strain: Δ(ara-leu) 7697 araD139 fhuA ΔlacX74 galK16 galE15 e14-  ϕ80dlacZΔM15  recA1 relA1 endA1 nupG  rpsL 

This plasmid was created in this work from pKT25

This plasmid is based on pKT25 (Euromedex): Bacterial two-hybrid vector designed to express a given polypeptide fused in frame at its N-terminal end with T25 fragment; p15 ori, KmR

Kanamycin resistant

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