TraVF was PCR amplified using primer pair 59/60 and cloned into pUTM18CDraft
This plasmid was constructed in this work: Protein interactions within and between two F-type type IV secretion systems. Birgit Koch, Melanie M. Callaghan, Jonathan Tellechea Luzardo, Ami Y. Seeger, Joseph P. Dillard, and Natalio Krasnogor. 2020. Submitted to Molecular Microbiology. The backbone (pUTM18C) comes from: Ouellette, S.P., Gauliard, E., Antosová, Z., and Ladant, D. (2014) A Gateway ® -compatible bacterial adenylate cyclase-based two-hybrid system: A Gateway-compatible bacterial two-hybrid system. Environ Microbiol Rep 6: 259–267.
This plasmid was propagated using 10-beta strain: Δ(ara-leu) 7697 araD139 fhuA ΔlacX74 galK16 galE15 e14- ϕ80dlacZΔM15 recA1 relA1 endA1 nupG rpsL
This plasmid is based on pUT18MC (Ouellette et al., 2014): As pUT18C but designed to insert the TM domain 1 of E. coli oppB between the cloned polypeptide and the T18 fragment; ColE1 ori, AmpR.
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