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JonathanT

Last updated: Aug 04, 2021

BW25113 - wild type

This is the wild type strain used for barcoding purposes in E. coli

The script used to find the barcoding site (essential_pair_finder.py) is attached. The script uses as input the .gff3 annotation file (BW25113.gff3) and the essential genes file (keio_genes_synonimschanged.txt). 

It prints as output the closest pair of essential genes.

Commited
Whole genome sequence

Datsenko, KA and Wanner, BL (2000) One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc. Natl. Acad. Sci. U.S.A. 97:6640-5

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Strain data
Barcode
v1 fe7c16

fe7c1691ee1c4bcda1444aaa4a3bfaf9b2c357b35

Escherichia coli

ATCC 11775

BW25113

The initial aliquot of BW25113 cells was obtained from our strain collection in Devonshire Building.

rrnB3 ΔlacZ4787 hsdR514 Δ(araBAD)567 Δ(rhaBAD)568 rph-1.

Newcastle University, Devonshire Building. ICOS -80 room.