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JonathanT
Last updated: Aug 04, 2021
BW25113 - BC383
This is the barcoded version of BW25113 using CRISPR as barcoding method (Li et al. 2015). The attached plasmid contains the gRNA and the repair template.
CommitedThis strain was produced in this study using the procedure described in: Li Y, Lin Z, Huang C, et al. Metabolic engineering of Escherichia coli using CRISPR-Cas9 meditated genome editing. Metab Eng. 2015;31:13‐21. doi:10.1016/j.ymben.2015.06.006
Strain data
Barcode
Escherichia coli
ATCC 11775s316
from parent
rrnB3 ΔlacZ4787 hsdR514 Δ(araBAD)567 Δ(rhaBAD)568 rph-1. BC383 (in between pyrH and frr)
Devonshire Building, Newcastle University. ICOS -80 room.
Apr 16, 2019