BW25113 - BC383
This is the barcoded version of BW25113 using CRISPR as barcoding method (Li et al. 2015). The attached plasmid contains the gRNA and the repair template.Commited
This strain was produced in this study using the procedure described in: Li Y, Lin Z, Huang C, et al. Metabolic engineering of Escherichia coli using CRISPR-Cas9 meditated genome editing. Metab Eng. 2015;31:13‐21. doi:10.1016/j.ymben.2015.06.006
Escherichia coliATCC 11775
rrnB3 ΔlacZ4787 hsdR514 Δ(araBAD)567 Δ(rhaBAD)568 rph-1. BC383 (in between pyrH and frr)
Devonshire Building, Newcastle University. ICOS -80 room.
Apr 16, 2019