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JonathanT

Last updated: Aug 04, 2021

BW25113 - CmR- BC383

This is the barcoded version of BW25113 using lambda-red as barcoding method (Datsenko, KA and Wanner, BL 2000). The DNA cassette PCR amplified from pEC-RED2 (attached). 

Commited
Whole genome sequence

This strain was produced in this study using the procedure described in:Datsenko, KA and Wanner, BL (2000) One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc. Natl. Acad. Sci. U.S.A. 97:6640-5

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Strain data
Barcode
v1 8328b5

8328b553bfd349f098d19ce9d95e05d6b2c0ad6eb

v1 GTCGTG

GTCGTGTCTGTCAATATCGGGGATAATAAATGTAGATAATGATTTCGTCAAGTCCTTACCCCGACTATACAACAGCAATCCATAGTAATCTGTGCGAATGGTTTAGTAGGACATAACACAAAT

Escherichia coli

ATCC 11775

s279

from parent

rrnB3 ΔlacZ4787 hsdR514 Δ(araBAD)567 Δ(rhaBAD)568 rph-1. CmR-BC383 (in between pyrH and frr)

Chloramphenicol resistance

Newcastle University, Devonshire Building. ICOS -80 room.

Mar 01, 2019