BW25113 - CmR- BC383
This is the barcoded version of BW25113 using lambda-red as barcoding method (Datsenko, KA and Wanner, BL 2000). The DNA cassette PCR amplified from pEC-RED2 (attached).Commited
This strain was produced in this study using the procedure described in:Datsenko, KA and Wanner, BL (2000) One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc. Natl. Acad. Sci. U.S.A. 97:6640-5
Escherichia coliATCC 11775
rrnB3 ΔlacZ4787 hsdR514 Δ(araBAD)567 Δ(rhaBAD)568 rph-1. CmR-BC383 (in between pyrH and frr)
Newcastle University, Devonshire Building. ICOS -80 room.
Mar 01, 2019