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JonathanT
Last updated: Aug 04, 2021
BW25113 - BC383
This is the barcoded version of BW25113 using lambda-red as barcoding method (Datsenko, KA and Wanner, BL 2000). CamR was removed by FLP expression (pCP20).
CommitedDatsenko, KA and Wanner, BL (2000) One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc. Natl. Acad. Sci. U.S.A. 97:6640-5
Strain data
Barcode
Escherichia coli
ATCC 11775s284
from parent
rrnB3 ΔlacZ4787 hsdR514 Δ(araBAD)567 Δ(rhaBAD)568 rph-1. BC383 (in between pyrH and frr)
Newcastle University, Devonshire Building. ICOS -80 room.
Mar 01, 2019
