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JonathanT
Last updated: Aug 05, 2021
168 - ZeoR - BC659
This is the barcoded version of 168 using Cre-Lox as barcoding method (Koo et al, 2016). The DNA cassette was PCR amplified from pBS-CreLox2-BC (attached).
CommitedThis strain was produced in this study using the procedure described in: B. M. Koo et al., “Construction and Analysis of Two Genome-Scale Deletion Libraries for Bacillus subtilis,” Cell Syst., vol. 22, pp. 291–305, 2017, doi: 10.1016/j.cels.2016.12.013.
Strain data
Barcode
Bacillus subtilis
ATCC 6051s289
from parent
trpC2, ZeoR-BC659 (in between rpsD and tyrS genes)
Trp(-), Zeocin resistant
Newcastle University, Devonshire Building. ICOS -80 room.
Mar 01, 2019