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JonathanT

Last updated: Aug 05, 2021

168 - ZeoR - BC659

This is the barcoded version of 168 using Cre-Lox as barcoding method (Koo et al, 2016). The DNA cassette was PCR amplified from pBS-CreLox2-BC (attached). 

Commited
Whole genome sequence

This strain was produced in this study using the procedure described in: B. M. Koo et al., “Construction and Analysis of Two Genome-Scale Deletion Libraries for Bacillus subtilis,” Cell Syst., vol. 22, pp. 291–305, 2017, doi: 10.1016/j.cels.2016.12.013.

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Strain data
Barcode
v1 dcabd7

dcabd7cccc09420682210d4384131e65b2c2242df

v1 CTTACT

Genosignature: CTTACTATGCCACATAGTCTAACTACTTTGCGTAGGTAATAGAGCAACGAGTAGGGTTATCGTCTTTCATACTCGGTTGGCGATTATACAAACTCCAATGGTTTGATTGGTGTTTCTCATACG

Show GC content diagram

Bacillus subtilis

ATCC 6051

s289

from parent

trpC2, ZeoR-BC659 (in between rpsD and tyrS genes)

Trp(-), Zeocin resistant

Newcastle University, Devonshire Building. ICOS -80 room.

Mar 01, 2019