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JonathanT

Last updated: Aug 05, 2021

168 - BC659

This is the barcoded version of 168 using Cre-Lox as barcoding method (Koo et al, 2016). The resistance marker was removed from its parental strain using Cre mediated recombination using pDR244.

Commited
Whole genome sequence

This strain was produced in this study using the procedure described in: B. M. Koo et al., “Construction and Analysis of Two Genome-Scale Deletion Libraries for Bacillus subtilis,” Cell Syst., vol. 22, pp. 291–305, 2017, doi: 10.1016/j.cels.2016.12.013.

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Strain data
Barcode
v1 dcabd7

dcabd7cccc09420682210d4384131e65b2c2242df

v1 ⎆CTTACT

CTTACTATGCCACATAGTCTAACTACTTTGCGTAGGTAATAGAGCAACGAGTAGGGTTATCGTCTTTCATACTCGGTTGGCGATTATACAAACTCCAATGGTTTGATTGGTGTTTCTCATACG

Show GC content diagram

Bacillus subtilis

ATCC 6051

s294

from parent

trpC2, BC659 (in between rpsD and tyrS genes)

Trp(-)

Newcastle University, Devonshire Building. ICOS -80 room.

Mar 01, 2019