Loading...

JonathanT
Last updated: Aug 05, 2021
168 - BC659
This is the barcoded version of 168 using Cre-Lox as barcoding method (Koo et al, 2016). The resistance marker was removed from its parental strain using Cre mediated recombination using pDR244.
CommitedThis strain was produced in this study using the procedure described in: B. M. Koo et al., “Construction and Analysis of Two Genome-Scale Deletion Libraries for Bacillus subtilis,” Cell Syst., vol. 22, pp. 291–305, 2017, doi: 10.1016/j.cels.2016.12.013.
Strain data
Barcode
Bacillus subtilis
ATCC 6051s294
from parent
trpC2, BC659 (in between rpsD and tyrS genes)
Trp(-)
Newcastle University, Devonshire Building. ICOS -80 room.
Mar 01, 2019