168 - BC659
This is the barcoded version of 168 using the toxin-mediated recombination as barcoding method (Lin et al, 2013). The DNA cassette was PCR amplified from the in-vitro assembly of all the parts.Commited
This strain was produced in this study using the procedure described in: Z. Lin et al., “A versatile mini-mazF-cassette for marker-free targeted genetic modification in Bacillus subtilis,” J. Microbiol. Methods, vol. 95, pp. 207–214, 2013, doi: 10.1016/j.mimet.2013.07.020.
Bacillus subtilisATCC 6051
trpC2, BC659 (in between rpsD and tyrS genes)
Newcastle University, Devonshire Building. ICOS -80 room.
Nov 04, 2019