168 - BC659
This is the barcoded version of 168 using CRISPR as barcoding method (J. Altenbuchner 2016). pBS-CRISPR2-BC was used to barcode this strain.Commited
This strain was produced in this study using the procedure described in: J. Altenbuchner, “Editing of the Bacillus subtilis Genome by the CRISPR-Cas9 System.,” Appl. Environ. Microbiol., vol. 82, no. 17, pp. 5421–7, Sep. 2016, doi: 10.1128/AEM.01453-16.
Bacillus subtilisATCC 6051
trpC2, BC659 (in between rpsD and tyrS genes)
Newcastle University, Devonshire Building. ICOS -80 room.
Nov 05, 2019