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JonathanT
Last updated: Aug 05, 2021
168 - BC659
This is the barcoded version of 168 using CRISPR as barcoding method (J. Altenbuchner 2016). pBS-CRISPR2-BC was used to barcode this strain.
CommitedThis strain was produced in this study using the procedure described in: J. Altenbuchner, “Editing of the Bacillus subtilis Genome by the CRISPR-Cas9 System.,” Appl. Environ. Microbiol., vol. 82, no. 17, pp. 5421–7, Sep. 2016, doi: 10.1128/AEM.01453-16.
Strain data
Barcode
Bacillus subtilis
ATCC 6051s356
from parent
trpC2, BC659 (in between rpsD and tyrS genes)
Trp(-)
Newcastle University, Devonshire Building. ICOS -80 room.
Nov 05, 2019