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JonathanT
Last updated: Sep 01, 2021
EM383 - BC6
This is the barcoded version of EM383 using CRISPR/targetron as barcoding method (Velazquez et al, 2019).
CommitedThis strain was produced by collaborators using the procedure described in: E. Velázquez, V. de Lorenzo, and Y. Al-Ramahi, “Recombination-Independent Genome Editing through CRISPR/Cas9-Enhanced TargeTron Delivery,” ACS Synth. Biol., vol. 8, no. 9, pp. 2186–2193, Sep. 2019, doi: 10.1021/acssynbio.9b00293.
Strain data
Barcode
Pseudomonas putida
ATCC 12633s369
Obtained from collaborators (see parent)
Δprophage1 Δprophage4 Δprophage3 Δprophage2 ΔTn7 ΔendA-1 ΔendA-2 ΔhsdRMS Δflagellum ΔTn4652 ΔrecA. BC6 (upstream of glmS gene)
Newcastle University, Devonshire Building. ICOS -80 room.
Nov 25, 2019
