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JonathanT
Last updated: Sep 01, 2021
J1074 - BC4
This is the barcoded version of J1074 using CRISPR as barcoding method (Cobb et al, 2015). The barcoding plasmid used was pSA-CRISPR-BC (attached).
CommitedThis strain was produced in this study using the procedure described in: Cobb RE, Wang Y, Zhao H. High-efficiency multiplex genome editing of Streptomyces species using an engineered CRISPR/Cas system. ACS Synth Biol. 2015;4(6):723-728. doi:10.1021/sb500351f
Strain data
Barcode
Streptomyces albidoflavus
ATCC 25422s268
from parent
BC4 (inserted in between XNR_3871 and XNR_3872)
Newcastle University, Devonshire Building. ICOS -80 room.
Feb 05, 2019