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JonathanT

Last updated: Sep 02, 2021

GS115 - BC10

This is the barcoded version of GS115 using Thermo Fischer AOX1 insertion protocol as barcoding method.

Commited
Digital Barcode (Version 1.0)

2074106e6e5a472bbdd60b3705146446b2c87c045

Physical Barcode (Version 1.0)
TAGAGCAAGTAATATGTAGCGATAGCGTGGGGGGAATAAAGTTAGTCGCCACTTTGTCGGGTAGCAGATAGTGTAATCTATTCAAACTCATAAAACTCGTCTTTATGCCCTTTGGTATCAATC

Thermo Fisher Scientific’s pPICZ A, B, and C transformation protocol (Manual part no. 25-0148)

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Strain data

Komagataella phaffii

ATCC 20864

s256

from parent

his4, ZeoR-BC10 (downstream of AOX1 gene)

Auxotrophic for histidine, Zeocin resistant

Newcastle University, Devonshire Building. ICOS -80 room.

Jan 11, 2019