BY4742 - BC2
This is the barcoded version of BY4742 using Cre-Lox as barcoding method (Fang 2011). The resistance marker was removed from its parental strain using Cre mediated recombination using pBF3060_NatMX (attached).Commited
Fang F, Salmon K, Shen MW, et al. A vector set for systematic metabolic engineering in Saccharomyces cerevisiae. Yeast. 2011;28(2):123-136. doi:10.1002/yea.1824
Saccharomyces cerevisiaeDSMZ DSM-70449
S288C-derivative laboratory strain: MATα his3Δ1 leu2Δ0 lys2Δ0 ura3Δ0. BC2 (inserted between NCA3 and ASF1)
Auxotrophic for: histidine, leucine, lysine and uracil.
Devonshire Building, Newcastle University. ICOS -80 room.
Jun 25, 2019