LOCUS       pEC-CRISPR2-BC          3372 bp ds-DNA     circular     15-NOV-2019
DEFINITION  .
SOURCE      Cloning vector pUC19                                               
  ORGANISM  Cloning vector pUC19 other sequences; artificial sequences;        
            vectors.
FEATURES             Location/Qualifiers
     CDS             2025..2064
                     /label="spy-tem"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     gene            complement(join(3020..3372,1..508))
                     /label="bla gene"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    1273..1395
                     /label="BC-383"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    1880..1899
                     /label="BC_44"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    1397..1879
                     /label="RH"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_RNA        2703..2810
                     /label="RNAI transcript"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     CDS             1928..1956
                     /label="pJ23119"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_RNA        complement(2261..2813)
                     /label="RNAII transcript (complementary strand)"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    1398..1449
                     /label="putative promoter frr"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    complement(773..791)
                     /label="pUC_EX_rev"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     CDS             complement(join(3020..3372,1..508))
                     /label="bla CDS"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     -35_signal      complement(2844..2849)
                     /label="RNAII promoter (counter-clockwise); TTGAGA"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     rep_origin      complement(2261..2849)
                     /label="pMB1 origin of replication (counter-clockwise)(RNAII -35 to RNA/DNA switch point)"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    1963..1982
                     /label="Ec - gRNA 1"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    1248..1272
                     /label="Universal primer"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    complement(2122..2141)
                     /label="Rvs"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     -10_signal      2689..2694
                     /label="RNAI promoter (clockwise); GCTACA"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    2142..2162
                     /label="pUC_SP_fw"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    1912..1931
                     /label="Fw-KpnI"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    792..1246
                     /label="LH"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     -10_signal      complement(552..557)
                     /label="bla promoter (counter-clockwise); GAGACA"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    1514..1879
                     /label="frr"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    792..1212
                     /label="pyrH"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     -35_signal      complement(573..578)
                     /label="bla promoter (counter-clockwise); TTCAAA"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     -10_signal      complement(2823..2828)
                     /label="RNAII promoter (counter-clockwise); CGTAAT"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    1938..2007
                     /label="oligo"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     Primer          1983..2006
                     /label="Fwd_primer"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             1983..2024
                     /label="SP_scaffold"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     Primer          complement(1938..1962)
                     /label="Rev_primer"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     -35_signal      2667..2672
                     /label="RNAI promoter (clockwise); TTGAAG"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     sig_peptide     complement(440..508)
                     /label="bla sig_peptide"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer          complement(2122..2166)
                     /label="BC_174"
                     /note="sequence: accgtattaccgcctttgagtgagctggagctgcacatgaactcg"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer          767..811
                     /label="BC281"
                     /note="sequence: cacagcttgtctgtaagcggatgccTCGTCTGATGTCCGCTATTC"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer          complement(1861..1904)
                     /label="BC284"
                     /note="sequence: ataccgcacagatgcgtaaggagaaTTCTGCTTCACCACGAACG"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
ORIGIN
        1 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
       61 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
      121 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
      181 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
      241 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
      301 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
      361 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
      421 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
      481 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
      541 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
      601 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta
      661 tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg
      721 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt
      781 aagcggatgc cTCGTCTGAT GTCCGCTATT CCATTGAATG GCGTGTGCGA CAGCTACAGC
      841 TGGGCAGAAG CTATCAGCCT GTTGCGCAAC AACCGTGTGG TGATCCTCTC CGCCGGTACA
      901 GGTAACCCGT TCTTTACCAC CGACTCAGCA GCTTGCCTGC GTGGTATCGA AATTGAAGCC
      961 GATGTGGTGC TGAAAGCAAC CAAAGTTGAC GGCGTGTTTA CCGCTGATCC GGCGAAAGAT
     1021 CCAACCGCAA CCATGTACGA GCAACTGACT TACAGCGAAG TGCTGGAAAA AGAGCTGAAA
     1081 GTCATGGACC TGGCGGCCTT CACGCTGGCT CGTGACCATA AATTACCGAT TCGTGTTTTC
     1141 AATATGAACA AACCGGGTGC GCTGCGCCGT GTGGTAATGG GTGAAAAAGA AGGGACTTTA
     1201 ATCACGGAAT AATTCCCGTG ATGGATAAAT AAGGGTAAGA TTCCGCtTGG ACATACATAG
     1261 TATACTCTGG TGGTCGTGTC TGTCAATATC GGGGATAATA AATGTAGATA ATGATTTCGT
     1321 CAAGTCCTTA CCCCGACTAT ACAACAGCAA TCCATAGTAA TCTGTGCGAA TGGTTTAGTA
     1381 GGACATAACA CAAATaATAG CCTAATAATC CAGACGATTA CCCGTAATAT GTTTAATCAG
     1441 GGCTATACTT AGCACACTTC CACTGTGTGT GACTGTCTGG TCTGACTGAG ACAAGTTTTC
     1501 AAGGATTCGT AACGTGATTA GCGATATCAG AAAAGATGCT GAAGTACGCA TGGACAAATG
     1561 CGTAGAAGCG TTCAAAACCC AAATCAGCAA AATACGCACG GGTCGTGCTT CTCCCAGCCT
     1621 GCTGGATGGC ATTGTCGTGG AATATTACGG CACGCCGACG CCGCTGCGTC AGCTGGCAAG
     1681 CGTAACGGTA GAAGATTCCC GTACACTGAA AATCAACGTG TTTGATCGTT CAATGTCTCC
     1741 GGCCGTTGAA AAAGCGATTA TGGCGTCCGA TCTTGGCCTG AACCCGAACT CTGCGGGTAG
     1801 CGACATCCGT GTTCCGCTGC CGCCGCTGAC GGAAGAACGT CGTAAAGATC TGACCAAAAT
     1861 CGTTCGTGGT GAAGCAGAAt tctccttacg catctgtgcg gtatttcaca ccgcatatgc
     1921 tggatccttg acagctagct cagtcctagg tataatacta gtATTCCGCG TAAGTATCGC
     1981 GGgttttaga gctagaaata gcaagttaaa ataaggctag tccgttatca acttgaaaaa
     2041 gtggcaccga gtcggtgctt tttttgaatt ctctagagtc gacctgcaga agcttagatc
     2101 tattaccctg ttatccctac tcgagttcat gtgcagctcc agctcactca aaggcggtaa
     2161 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     2221 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     2281 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     2341 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     2401 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     2461 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     2521 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     2581 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     2641 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     2701 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     2761 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     2821 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     2881 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     2941 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     3001 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     3061 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     3121 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     3181 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     3241 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     3301 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     3361 cgtttggtat gg
//