LOCUS       pEC-Red2-BC             3697 bp ds-DNA     circular     15-NOV-2019
DEFINITION  .
SOURCE      synthetic DNA construct                                            
  ORGANISM  synthetic DNA construct                                            
FEATURES             Location/Qualifiers
     misc_feature    505..627
                     /label="BC-383"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    1584..1949
                     /label="frr"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     terminator      3643..3670
                     /label="rrnB T2 terminator"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     CDS             complement(2409..3269)
                     /label="AmpR"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    628..661
                     /label="End signal"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    24..444
                     /label="pyrH"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    24..478
                     /label="LH"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer_bind     3032..3051
                     /label="Amp-R"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    480..504
                     /label="Universal primer"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     rep_origin      1956..2312
                     /label="R6K gamma ori"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_recomb     628..661
                     /label="FRT"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     CDS             complement(1104..1322)
                     /label="cat CDS"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     promoter        complement(3270..3374)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     terminator      3465..3551
                     /label="rrnB T1 terminator"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    1467..1949
                     /label="RH"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    1433..1466
                     /label="FRT"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     -35_signal      complement(1412..1417)
                     /label="cat promoter (counter-clockwise); ACGTAA"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     CDS             complement(663..1103)
                     /label="cat CDS"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     gene            complement(1104..1322)
                     /label="cat gene"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    1468..1519
                     /label="putative promoter frr"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     -10_signal      complement(1389..1394)
                     /label="cat promoter (counter-clockwise); CATATT"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer          complement(1409..1463)
                     /label="BC278"
                     /note="sequence: gttcctatactttctagagaataggaacttcgagacgttgatcggcacgtaagag"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          1..43
                     /label="BC275"
                     /note="sequence: agattgcagcattacacgtcttgTCGTCTGATGTCCGCTATTC"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer          complement(1931..1974)
                     /label="BC280"
                     /note="sequence: gaacacttaacggctgacatgggaaTTCTGCTTCACCACGAACG"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer          complement(2..23)
                     /label="BC273"
                     /note="sequence: caagacgtgtaatgctgcaatc"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          1439..1492
                     /label="BC279"
                     /note="sequence: cctattctctagaaagtataggaacttcATAGCCTAATAATCCAGACGATTACC"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer          1950..1969
                     /label="BC274"
                     /note="sequence: ttcccatgtcagccgttaag"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer          663..677
                     /label="BC277"
                     /note="sequence: ttacgccccgccctg"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
ORIGIN
        1 agattgcagc attacacgtc ttgTCGTCTG ATGTCCGCTA TTCCATTGAA TGGCGTGTGC
       61 GACAGCTACA GCTGGGCAGA AGCTATCAGC CTGTTGCGCA ACAACCGTGT GGTGATCCTC
      121 TCCGCCGGTA CAGGTAACCC GTTCTTTACC ACCGACTCAG CAGCTTGCCT GCGTGGTATC
      181 GAAATTGAAG CCGATGTGGT GCTGAAAGCA ACCAAAGTTG ACGGCGTGTT TACCGCTGAT
      241 CCGGCGAAAG ATCCAACCGC AACCATGTAC GAGCAACTGA CTTACAGCGA AGTGCTGGAA
      301 AAAGAGCTGA AAGTCATGGA CCTGGCGGCC TTCACGCTGG CTCGTGACCA TAAATTACCG
      361 ATTCGTGTTT TCAATATGAA CAAACCGGGT GCGCTGCGCC GTGTGGTAAT GGGTGAAAAA
      421 GAAGGGACTT TAATCACGGA ATAATTCCCG TGATGGATAA ATAAGGGTAA GATTCCGCTT
      481 GGACATACAT AGTATACTCT GGTGGTCGTG TCTGTCAATA TCGGGGATAA TAAATGTAGA
      541 TAATGATTTC GTCAAGTCCT TACCCCGACT ATACAACAGC AATCCATAGT AATCTGTGCG
      601 AATGGTTTAG TAGGACATAA CACAAATGAA GTTCCTATTC TCTAGAAAGT ATAGGAACTT
      661 CATTACGCCC CGCCCTGCCA CTCATCGCAG TACTGTTGTA ATTCATTAAG CATTCTGCCG
      721 ACATGGAAGC CATCACAAAC GGCATGATGA ACCTGAATCG CCAGCGGCAT CAGCACCTTG
      781 TCGCCTTGCG TATAATATTT GCCCATGGTG AAAACGGGGG CGAAGAAGTT GTCCATATTG
      841 GCCACGTTTA AATCAAAACT GGTGAAACTC ACCCAGGGAT TGGCTGAGAC GAAAAACATA
      901 TTCTCAATAA ACCCTTTAGG GAAATAGGCC AGGTTTTCAC CGTAACACGC CACATCTTGC
      961 GAATATATGT GTAGAAACTG CCGGAAATCG TCGTGGTATT CACTCCAGAG CGATGAAAAC
     1021 GTTTCAGTTT GCTCATGGAA AACGGTGTAA CAAGGGTGAA CACTATCCCA TATCACCAGC
     1081 TCACCGTCTT TCATTGCCAT ACGGAATTCC GGATGAGCAT TCATCAGGCG GGCAAGAATG
     1141 TGAATAAAGG CCGGATAAAA CTTGTGCTTA TTTTTCTTTA CGGTCTTTAA AAAGGCCGTA
     1201 ATATCCAGCT GAACGGTCTG GTTATAGGTA CATTGAGCAA CTGACTGAAA TGCCTCAAAA
     1261 TGTTCTTTAC GATGCCATTG GGATATATCA ACGGTGGTAT ATCCAGTGAT TTTTTTCTCC
     1321 ATTTTAGCTT CCTTAGCTCC TGAAAATCTC GATAACTCAA AAAATACGCC CGGTAGTGAT
     1381 CTTATTTCAT TATGGTGAAA GTTGGAACCT CTTACGTGCC GATCAACGTC TCGAAGTTCC
     1441 TATTCTCTAG AAAGTATAGG AACTTCATAG CCTAATAATC CAGACGATTA CCCGTAATAT
     1501 GTTTAATCAG GGCTATACTT AGCACACTTC CACTGTGTGT GACTGTCTGG TCTGACTGAG
     1561 ACAAGTTTTC AAGGATTCGT AACGTGATTA GCGATATCAG AAAAGATGCT GAAGTACGCA
     1621 TGGACAAATG CGTAGAAGCG TTCAAAACCC AAATCAGCAA AATACGCACG GGTCGTGCTT
     1681 CTCCCAGCCT GCTGGATGGC ATTGTCGTGG AATATTACGG CACGCCGACG CCGCTGCGTC
     1741 AGCTGGCAAG CGTAACGGTA GAAGATTCCC GTACACTGAA AATCAACGTG TTTGATCGTT
     1801 CAATGTCTCC GGCCGTTGAA AAAGCGATTA TGGCGTCCGA TCTTGGCCTG AACCCGAACT
     1861 CTGCGGGTAG CGACATCCGT GTTCCGCTGC CGCCGCTGAC GGAAGAACGT CGTAAAGATC
     1921 TGACCAAAAT CGTTCGTGGT GAAGCAGAAt tcccatgtca gccgttaagt gttcctgtgt
     1981 cactgaaaat tgctttgaga ggctctaagg gcttctcagt gcgttacatc cctggcttgt
     2041 tgtccacaac cgttaaacct taaaagcttt aaaagcctta tatattcttt tttttcttat
     2101 aaaacttaaa accttagagg ctatttaagt tgctgattta tattaatttt attgttcaaa
     2161 catgagagct tagtacgtga aacatgagag cttagtacgt tagccatgag agcttagtac
     2221 gttagccatg agggtttagt tcgttaaaca tgagagctta gtacgttaaa catgagagct
     2281 tagtacgtga aacatgagag cttagtacgt actatcaaca ggttgaactg cggatcttgc
     2341 ggccgcaaaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     2401 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     2461 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     2521 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     2581 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     2641 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     2701 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     2761 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     2821 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     2881 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     2941 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     3001 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     3061 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     3121 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     3181 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     3241 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     3301 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     3361 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg catcgatggc cccccgatgg
     3421 tagtgtgggg tctccccatg cgagagtagg gaactgccag gcatcaaata aaacgaaagg
     3481 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctcctga
     3541 gtaggacaaa tccgccggga gcggatttga acgttgcgaa gcaacggccc ggagggtggc
     3601 gggcaggacg cccgccataa actgccaggc atcaaattaa gcagaaggcc atcctgacgg
     3661 atggcctttt tgcgtggcca gtgccaagct tgcatgc
//