LOCUS       pBS-CRISPR_gRNA3_barcod 8692 bp ds-DNA     circular     13-JUL-2020
DEFINITION  .
ACCESSION   pJOE8999.1                                                         
SOURCE      Unknown.                                                           
  ORGANISM  Unknown Unclassified.                                              
FEATURES             Location/Qualifiers
     misc_feature    complement(3837..3865)
                     /label="tyrS ter"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             complement(4402..8504)
                     /label="cas9 CDS"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    3689..3713
                     /label="Universal primer"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    3165..3536
                     /label="rpsD"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             3101..3130
                     /label="oop ter CDS"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    3139..3164
                     /label="T7P misc_feature"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    2952..2971
                     /label="BS-gRNA3"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    2952..2953
                     /label="BS-gRNA2"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    3714..3836
                     /label="BC-659"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    4358..4383
                     /label="fw2"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    2972..2994
                     /label="fw"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    complement(58..731)
                     /label="pUCori misc_feature"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    complement(3147..3164)
                     /label="rev2"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    3165..3688
                     /label="LH"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     CDS             complement(831..1601)
                     /label="kanR CDS"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    3837..4357
                     /label="RH"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    complement(3871..4357)
                     /label="tyrS"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    complement(3558..3668)
                     /label="putative prote"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     CDS             complement(8534..8643)
                     /label="Pman CDS"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     promoter        2842..2951
                     /label="Pveg"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     CDS             2972..3052
                     /label="'gRNA CDS"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    complement(1716..2830)
                     /label="rep pE194ts misc_feature"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          3140..3183
                     /label="BC291"
                     /note="sequence: aatacgactcactatagggtcgacgCTAGCTGGTAAACACGGTG"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          complement(4336..4382)
                     /label="BC294"
                     /note="sequence: aagattatttcttaatctagaaaggCGACCGTGACGTTGTCAAATAC"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
ORIGIN
        1 gaatggccat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
       61 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
      121 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
      181 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
      241 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
      301 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
      361 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
      421 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
      481 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
      541 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
      601 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
      661 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
      721 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
      781 ttgagtgagc tgaaattatg aggggatctc tcagagctcg aggtcatcgt tcaaaatggt
      841 atgcgttttg acacatccac tatatatccg tgtcgttctg tccactcctg aatcccattc
      901 cagaaattct ctagcgattc cagaagtttc tcagagtcgg aaagttgacc agacattacg
      961 aactggcaca gatggtcata acctgaagga agatctgatt gcttaactgc ttcagttaag
     1021 accgaagcgc tcgtcgtata acagatgcga tgatgcagac caatcaacat ggcacctgcc
     1081 attgctacct gcacagtcaa ggatggtaga aatgttgtcg gtccttgcac acgaatatta
     1141 cgccatttgc ctgcatattc aaacagctct tctacgataa gggcacaaat cgcatcgtgg
     1201 aacgtttggg cttctaccga tttagcagtt tgatacactt tctctaagta tccacctgaa
     1261 tcataaatcg gcaaaataga gaaaaattga ccatgtgtaa gcggccaatc tgattccacc
     1321 tgagatgcat aatctagtag aatctcttcg ctatcaaaat tcacttccac cttccactca
     1381 ccggttgtcc attcatggct gaactctgct tcctctgttg acatgacaca catcatctca
     1441 atatccgaat agggcccatc agtctgacga ccaagagagc cataaacacc aatagcctta
     1501 acatcatccc catatttatc caatattcgt tccttaattt catgaacaat cttcattctt
     1561 tcttctctag tcattattat tggtccattc actattctca ttcccttttc agataatttt
     1621 agatttgctt ttctaaataa gaatatttgg agagcaccgt tcttattcag ctattaaacc
     1681 cattatatcg ggtttttgag gggatttcaa ctgcagacac ctaaattcaa aatctatcgg
     1741 tcagatttat accgatttga ttttatatat tcttgaataa catacgccga gttatcacat
     1801 aaaagcggga accaatcatc aaatttaaac ttcattgcat aatccattaa actcttaaat
     1861 tctacgattc cttgttcatc aataaactca atcatttctt taattaattt atatctatct
     1921 gttgttgttt tctttaataa ttcatcaaca tctacaccgc cataaactat catatcttct
     1981 ttttgatatt taaatttatt aggatcgtcc atgtgaagca tatatctcac aagacctttc
     2041 acacttcctg caatctgcgg aatagtcgca ttcaattctt ctgttaatta tttttatctg
     2101 ttcataagat ttattaccct catacatcac tagaatatga taatgctctt ttttcatcct
     2161 accttctgta tcagtatccc tatcatgtaa tggagacact acaaattgaa tgtgtaactc
     2221 ttttaaatac tctaaccact cggcttttgc tgattctgga tataaaacaa atgtccaatt
     2281 acgtcctctt gaatttttct tgttttcagt ttcttttatt acattttcgc tcatgatata
     2341 ataacggtgc taatacattt aacaaaattt agtcatagat aggcagcatg ccagtgctgt
     2401 ctatcttttt ttgtttaaaa tgcaccgtat tcctcctttg catatttttt tattagaata
     2461 ccggttgcat ctgatttgct aatattatat ttttctttga ttctatttaa tatctcattt
     2521 tcttctgttg taagtcttaa agtaacagca acttttttct cttcttttct atctacaacc
     2581 atcactgtac ctcccaacat ctgttttttt cactttaaca taaaaaacaa ccttttaaca
     2641 ttaaaaaccc aatatttatt tatttgtttg gacaatggac aatggacacc taggggggag
     2701 gtcgtagtac ccccctatgt tttctcccct aaataacccc aaaaatctaa gaaaaaaaga
     2761 cctcaaaaag gtctttaatt aacatctcaa atttcgcatt tattccaatt tcctttttgc
     2821 gtgtgatgcg aattcttgac cggatcctag aagcttatcg aattccttat taacgttgat
     2881 ataatttaaa ttttatttga caaaaatggg ctcgtgttgt acaataaatg taactttcag
     2941 tttagcggtc tGGAAAAGAG TATATTAGAT Agttttagag ctagaaatag caagttaaaa
     3001 taaggctagt ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt ttactccatc
     3061 tggatttgtt caaacgctcg gttgccgccg ggcgtttttt atctaaagct taggcccagt
     3121 cgaaagactg ggccttttta atacgactca ctatagggtc gacgCTAGCT GGTAAACACG
     3181 GTGAAAACTT CATGATTCTT TTAGATTCTC GTCTTGATAA CGTTGTGTAC AAGCTAGGTT
     3241 TAGCACGTAC TCGCCGTCAA GCTCGCCAAT TGGTTAACCA CGGTCACATT CTTGTAGACG
     3301 GAAGCCGCGT TGACATCCCG TCTTACCTAG TGAAACCTGG TCAAACAATC GGTGTTCGCG
     3361 AAAAATCAAG AAACCTTTCT ATCATCAAAG AATCTGTTGA AGTGAACAAC TTCGTTCCTG
     3421 AATACCTTAC TTTCGACGCT GAAAAGCTTG AAGGTACTTT CACTCGTCTT CCTGAGCGTT
     3481 CTGAACTTGC TCCGGAAATT AACGAAGCGC TTATCGTTGA GTTCTACTCT CGTTAATCGT
     3541 TTTAAAAACC CCTGCCGCTA TGCGGTCGGG GTTTTTTTAT CGGCTTTCGC CTCTTGCTGG
     3601 CCAACACTAT CTTGCGCCTG CTGCTCCCAA TGATCCCGCT GCAGCCGTTC TTTTGCAATC
     3661 GTACACATGA GATTCACGCT TTAGCGCTTG GACATACATA GTATACTCTG GTGCTTACTA
     3721 TGCCACATAG TCTAACTACT TTGCGTAGGT AATAGAGCAA CGAGTAGGGT TATCGTCTTT
     3781 CATACTCGGT TGGCGATTAT ACAAACTCCA ATGGTTTGAT TGGTGTTTCT CATACGGATC
     3841 CTTTGCCTTC AGTGGCAAAG GATCTTTTTC TTATTTATAC GTCACAAGGA AGTATTTTTT
     3901 CTTCCCGCGG CGCAGAACAG TAAATTGGTT TTCGATGCGG TCTTCACCTG ATAGGGTATA
     3961 ATTTATTTCC GTCTGGCGTT CACCGTTAAT GTAAACAGCT CCGTTTTGGA TGTCTTCACG
     4021 CGCTTGGCGT TTAGAAGGAG ATAATTTAGA TTGCACCAAC ACATCTACAA GTGAAAGCTC
     4081 TTGGGTGCTG TCAACTTCCA TAGAAGGAAC ATCTTTAAAG CCTACTTTTA CGTCTTGAGC
     4141 AGAAAGCTCT TTAATATTGC CGCTGAACAG CGCTTGGGAG ATGTTGATGG CTTGCTCCAG
     4201 CGCCTCGCGT CCGTGAACAA GTGATGTCAC TTCTTCTGCC AGGCGTTTTT GCGCTTCACG
     4261 TTTTTCTGGC GCCGTTTCTG TTTTTTCAGC GTATGCTTCA ATTTCCTCTT TTGAAAGGAA
     4321 CGTGAAGTAT TTCAAGTATT TGACAACGTC ACGGTCGcct ttctagatta agaaataatc
     4381 ttcatctaaa atatacttca gtcacctcct agctgactca aatcaatgcg tgtttcataa
     4441 agaccagtga tggattgatg gataagagtg gcatctaaaa cttcttttgt agacgtatat
     4501 cgtttacgat caattgttgt atcaaaatat ttaaaagcag cgggagctcc aagattcgtc
     4561 aacgtaaata aatgaataat attttctgct tgttcacgta ttggtttgtc tctatgtttg
     4621 ttatatgcac taagaacttt atctaaattg gcatctgcta aaataacacg cttagaaaat
     4681 tcactgattt gctcaataat ctcatctaaa taatgcttat gctgctccac aaacaattgt
     4741 ttttgttcgt tatcttctgg actacccttc aacttttcat aatgactagc taaatataaa
     4801 aaattcacat atttgcttgg cagagccagc tcatttcctt tttgtaattc tccggcacta
     4861 gccagcatcc gtttacgacc gttttctaac tcaaaaagac tatatttagg tagtttaatg
     4921 attaagtctt ttttaacttc cttatatcct ttagcttcta aaaagtcaat cggatttttt
     4981 tcaaaggaac ttctttccat aattgtgatc cctagtaact ctttaacgga ttttaacttc
     5041 ttcgatttcc ctttttccac cttagcaacc actaggactg aataagctac cgttggacta
     5101 tcaaaaccac catatttttt tggatcccag tcttttttac gagcaataag cttgtccgaa
     5161 tttctttttg gtaaaattga ctccttggag aatccgcctg tctgtacttc tgttttcttg
     5221 acaatattga cttggggcat ggacaatact ttgcgcactg tggcaaaatc tcgcccttta
     5281 tcccagacaa tttctccagt ttccccatta gtttcgatta gagggcgttt gcgaatctct
     5341 ccatttgcaa gtgtaatttc tgttttgaag aagttcatga tattagagta aaagaaatat
     5401 tttgcggttg ctttgcctat ttcttgctca gacttagcaa tcattttacg aacatcataa
     5461 actttataat caccatagac aaactccgat tcaagttttg gatatttctt aatcaaagca
     5521 gttccaacga cggcatttag atacgcatca tgggcatgat ggtaattgtt aatctcacgt
     5581 actttataga attggaaatc ttttcggaag tcagaaacta atttagattt taaggtaatc
     5641 actttaacct ctcgaataag tttatcattt tcatcgtatt tagtattcat gcgactatcc
     5701 aaaatttgtg ccacatgctt agtgatttgg cgagtttcaa ccaattggcg tttgataaaa
     5761 ccagctttat caagttcact caaacctcca cgttcagctt tcgttaaatt atcaaactta
     5821 cgttgagtga ttaacttggc gtttagaagt tgtctccaat agtttttcat ctttttgact
     5881 acttcttcac ttggaacgtt atccgattta ccacgatttt tatcagaacg cgttaagacc
     5941 ttattgtcta ttgaatcgtc tttaaggaaa ctttgtggaa caatgtgatc gacatcataa
     6001 tcacttaaac gattaatatc taattcttgg tccacataca tgtctcttcc attttggaga
     6061 taatagagat agagcttttc attttgcaat tgagtatttt caacaggatg ctctttaaga
     6121 atctgacttc ctaattcttt gataccttct tcgattcgtt tcatacgctc tcgcgaattt
     6181 ttctggccct tttgagttgt ctgattttca cgtgccattt caataacgat attttctggc
     6241 ttatgccgcc ccattacttt gaccaattca tcaacaactt ttacagtctg taaaatacct
     6301 tttttaatag cagggctacc agctaaattt gcaatatgtt catgtaaact atcgccttgt
     6361 ccagacactt gtgctttttg aatgtcttct ttaaatgtca aactatcatc atggatcagc
     6421 tgcataaaat tgcgattggc aaaaccatct gatttcaaaa aatctaatat tgttttgcca
     6481 gattgcttat ccctaatacc attaatcaat tttcgagaca aacgtcccca accagtataa
     6541 cggcgacgtt taagctgttt catcacctta tcatcaaaga ggtgagcata tgttttaagt
     6601 ctttcctcaa tcatctccct atcttcaaat aaggtcaatg ttaaaacaat atcctctaag
     6661 atatcttcat tttcttcatt atccaaaaaa tctttatctt taataatttt tagcaaatca
     6721 tggtaggtac ctaatgaagc attaaatcta tcttcaactc ctgaaatttc aacactatca
     6781 aaacattcta tttttttgaa ataatcttct tttaattgct taacggttac ttttcgattt
     6841 gttttgaaga gtaaatcaac aatggctttc ttctgttcac ctgaaagaaa tgctggtttt
     6901 cgcattcctt cagtaacata tttgaccttt gtcaattcgt tataaaccgt aaaatactca
     6961 taaagcaaac tatgttttgg tagtactttt tcatttggaa gatttttatc aaagtttgtc
     7021 atgcgttcaa taaatgattg agctgaagca cctttatcga caacttcttc aaaattccat
     7081 ggggtaattg tttcttcaga cttccgagtc atccatgcaa aacgactatt gccacgcgcc
     7141 aatggaccaa cataataagg aattcgaaaa gtcaagattt tttcaatctt ctcacgattg
     7201 tcttttaaaa atggataaaa gtcttcttgt cttctcaaaa tagcatgcag ctcacccaag
     7261 tgaatttgat ggggaataga gccgttgtca aaggtccgtt gcttgcgcag caaatcttca
     7321 cgatttagtt tcaccaataa ttcctcagta ccatccattt tttctaaaat tggtttgata
     7381 aatttataaa attcttcttg gctagctccc ccatcaatat aacctgcata tccgtttttt
     7441 gattgatcaa aaaagatttc tttatacttt tctggaagtt gttgtcgaac taaagctttt
     7501 aaaagagtca agtcttgatg atgttcatcg tagcgtttaa tcattgaagc tgatagggga
     7561 gccttagtta tttcagtatt tactcttagg atatctgaaa gtaaaatagc atctgataaa
     7621 ttcttagctg ccaaaaacaa atcagcatat tgatctccaa tttgcgccaa taaattatct
     7681 aaatcatcat cgtaagtatc ttttgaaagc tgtaatttag catcttctgc caaatcaaaa
     7741 tttgatttaa aattaggggt caaacccaat gacaaagcaa tgagattccc aaataagcca
     7801 tttttcttct caccggggag ctgagcaatg agattttcta atcgtcttga tttactcaat
     7861 cgtgcagaaa gaatcgcttt agcatctact ccacttgcgt taatagggtt ttcttcaaat
     7921 aattgattgt aggtttgtac caactggata aatagtttgt ccacatcact attatcagga
     7981 tttaaatctc cctcaatcaa aaaatgacca cgaaacttaa tcatatgcgc taaggccaaa
     8041 tagattaagc gcaaatccgc tttatcagta gaatctacca atttttttcg cagatgatag
     8101 atagttggat atttctcatg ataagcaact tcatctacta tatttccaaa aataggatga
     8161 cgttcatgct tcttgtcttc ttccaccaaa aaagactctt caagtcgatg aaagaaacta
     8221 tcatctactt tcgccatctc atttgaaaaa atctcctgta gataacaaat acgattcttc
     8281 cgacgtgtat accttctacg agctgtccgt ttgagacgag tcgcttccgc tgtctctcca
     8341 ctgtcaaata aaagagcccc tataagattt tttttgatac tgtggcggtc tgtatttccc
     8401 agaaccttga actttttaga cggaacctta taatcatcag tgatcaccgc ccatccgacg
     8461 ctatttgtgc cgatatctaa gcctattgag tatttcttat ccatttttgc ctcctaactt
     8521 aagaataaga tcttgtctca actgtatacc gaaatcagct cattaaaatc gcttttttta
     8581 ccataggttc cggtaataaa ggcatttttc cctataacaa aaaaagcaag gaataatccc
     8641 tgcttttaat aatccaaatg agataaaaat gtcatgacat tggtgtacag aa
//