LOCUS       pBS-CreLox2-BC          3645 bp ds-DNA     circular     15-NOV-2019
DEFINITION  .
ACCESSION   pTarget                                                            
SOURCE      Unknown.                                                           
  ORGANISM  Unknown Unclassified.                                              
FEATURES             Location/Qualifiers
     misc_feature    complement(1276..1386)
                     /label="putative prote"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    883..1254
                     /label="rpsD"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    complement(2067..2095)
                     /label="tyrS ter"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    863..882
                     /label="Fwd"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    2033..2066
                     /label="lox66"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             75..663
                     /label="pMB1"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    1555..2066
                     /label="lox71-zeocin-lox66"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     CDS             2754..3546
                     /label="aadA_spec/strep_resistance"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     rep_origin      join(3644..3645,1..681)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    883..1406
                     /label="LH"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    complement(2632..2651)
                     /label="Rvs"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    complement(762..782)
                     /label="Rev-SphI"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    complement(2101..2587)
                     /label="tyrS"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    2067..2587
                     /label="RH"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    1555..1588
                     /label="lox71"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    1407..1431
                     /label="Universal primer"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    complement(2772..2792)
                     /label="SEQ and PCR"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    1432..1554
                     /label="BC-659"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer          2038..2085
                     /label="BC289"
                     /note="sequence: ttcgtatagcatacattatacgaacggtaGATCCTTTGCCTTCAGTGG"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer          complement(2016..2062)
                     /label="BC150"
                     /note="sequence: gttcgtataatgtatgctatacgaagttattcagtcctgctcctcgg"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          1559..1614
                     /label="BC149"
                     /note="sequence: gttcgtatagcatacattatacgaagttatgtttttcatgaaagacttgatatggc"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
ORIGIN
        1 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
       61 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
      121 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
      181 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag
      241 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
      301 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
      361 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
      421 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
      481 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
      541 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
      601 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
      661 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
      721 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
      781 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
      841 gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcCTAGCTGG TAAACACGGT
      901 GAAAACTTCA TGATTCTTTT AGATTCTCGT CTTGATAACG TTGTGTACAA GCTAGGTTTA
      961 GCACGTACTC GCCGTCAAGC TCGCCAATTG GTTAACCACG GTCACATTCT TGTAGACGGA
     1021 AGCCGCGTTG ACATCCCGTC TTACCTAGTG AAACCTGGTC AAACAATCGG TGTTCGCGAA
     1081 AAATCAAGAA ACCTTTCTAT CATCAAAGAA TCTGTTGAAG TGAACAACTT CGTTCCTGAA
     1141 TACCTTACTT TCGACGCTGA AAAGCTTGAA GGTACTTTCA CTCGTCTTCC TGAGCGTTCT
     1201 GAACTTGCTC CGGAAATTAA CGAAGCGCTT ATCGTTGAGT TCTACTCTCG TTAATCGTTT
     1261 TAAAAACCCC TGCCGCTATG CGGTCGGGGT TTTTTTATCG GCTTTCGCCT CTTGCTGGCC
     1321 AACACTATCT TGCGCCTGCT GCTCCCAATG ATCCCGCTGC AGCCGTTCTT TTGCAATCGT
     1381 ACACATGAGA TTCACGCTTT AGCGCTTGGA CATACATAGT ATACTCTGGT GCTTACTATG
     1441 CCACATAGTC TAACTACTTT GCGTAGGTAA TAGAGCAACG AGTAGGGTTA TCGTCTTTCA
     1501 TACTCGGTTG GCGATTATAC AAACTCCAAT GGTTTGATTG GTGTTTCTCA TACGtaccgt
     1561 tcgtatagca tacattatac gaagttatgt ttttcatgaa agacttgata tggcttttta
     1621 tatgtgttac tctacataca gaaaggagga actaaatatg gccaagttga ccagtgccgt
     1681 tccggtgctc accgcgcgcg acgtcgccgg agcggtcgag ttctggaccg accggctcgg
     1741 gttctcccgg gacttcgtgg aggacgactt cgccggtgtg gtccgggacg acgtgaccct
     1801 gttcatcagc gcggtccagg accaggtggt gccggacaac accctggcct gggtgtgggt
     1861 gcgcggcctg gacgagctgt acgccgagtg gtcggaggtc gtgtccacga acttccggga
     1921 cgcctccggg ccggccatga ccgagatcgg cgagcagccg tgggggcggg agttcgccct
     1981 gcgcgacccg gccggcaact gcgtgcactt cgtggccgag gagcaggact gaataacttc
     2041 gtatagcata cattatacga acggtaGATC CTTTGCCTTC AGTGGCAAAG GATCTTTTTC
     2101 TTATTTATAC GTCACAAGGA AGTATTTTTT CTTCCCGCGG CGCAGAACAG TAAATTGGTT
     2161 TTCGATGCGG TCTTCACCTG ATAGGGTATA ATTTATTTCC GTCTGGCGTT CACCGTTAAT
     2221 GTAAACAGCT CCGTTTTGGA TGTCTTCACG CGCTTGGCGT TTAGAAGGAG ATAATTTAGA
     2281 TTGCACCAAC ACATCTACAA GTGAAAGCTC TTGGGTGCTG TCAACTTCCA TAGAAGGAAC
     2341 ATCTTTAAAG CCTACTTTTA CGTCTTGAGC AGAAAGCTCT TTAATATTGC CGCTGAACAG
     2401 CGCTTGGGAG ATGTTGATGG CTTGCTCCAG CGCCTCGCGT CCGTGAACAA GTGATGTCAC
     2461 TTCTTCTGCC AGGCGTTTTT GCGCTTCACG TTTTTCTGGC GCCGTTTCTG TTTTTTCAGC
     2521 GTATGCTTCA ATTTCCTCTT TTGAAAGGAA CGTGAAGTAT TTCAAGTATT TGACAACGTC
     2581 ACGGTCGgtc gacctgcaga agcttagatc tattaccctg ttatccctac tcgagttcat
     2641 gtgcagctcc atcagcaaaa ggggatgata agtttatcac caccgactat ttgcaacagt
     2701 gccgttgatc gtgctatgat cgactgatgt catcagcggt ggagtgcaat gtcatgaggg
     2761 aagcggtgat cgccgaagta tcgactcaac tatcagaggt agttggcgtc atcgagcgcc
     2821 atctcgaacc gacgttgctg gccgtacatt tgtacggctc cgcagtggat ggcggcctga
     2881 agccacacag tgatattgat ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc
     2941 ggcgagcttt gatcaacgac cttttggaaa cttcggcttc ccctggagag agcgagattc
     3001 tccgcgctgt agaagtcacc attgttgtgc acgacgacat cattccgtgg cgttatccag
     3061 ctaagcgcga actgcaattt ggagaatggc agcgcaatga cattcttgca ggtatcttcg
     3121 agccagccac gatcgacatt gatctggcta tcttgctgac aaaagcaaga gaacatagcg
     3181 ttgccttggt aggtccagcg gcggaggaac tctttgatcc ggttcctgaa caggatctat
     3241 ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg gctggcgatg
     3301 agcgaaatgt agtgcttacg ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg
     3361 cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct gccggcccag tatcagcccg
     3421 tcatacttga agctagacag gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg
     3481 cagatcagtt ggaagaattt gtccactacg tgaaaggcga gatcaccaag gtagtcggca
     3541 aataagatgc cgctcgccag tcgattggct gagctcatga agttcctatt ccgaagttcc
     3601 gcgaacgcgt aaaggatcta ggtgaagatc ctttttgata atctc
//