LOCUS       pKT25TraVNG_(OK)        3968 bp ds-DNA     circular     09-JUL-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    join(3060..3968,1..5)
                     /label="p15A"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer_bind     309..329
                     /label="M13-rev"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    complement(1973..2773)
                     /label="KanR"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    1020..1544
                     /label="traV-NG"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     CDS             1662..1730
                     /label="LacZ alpha"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_binding    281..303
                     /label="LacO"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    321..998
                     /label="TM25"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    215..227
                     /label="CAP"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    160..276
                     /label="Plac promoter"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     promoter        247..277
                     /label="lac promoter"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    1719..1761
                     /label="Terminator"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
ORIGIN
        1 Aagctttaat gcggtagttt atcacagtta aattgctaac gcagtcaggc accgtgtatg
       61 aaatctaaca atgcgctcat cgtcatcctc ggcaccgtca ccctggatgc tgtaggcata
      121 ggcttggtta tgccggtact gccgggcctc ttgcgggatc tggcacgaca ggtttcccga
      181 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
      241 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
      301 atttcacaca ggaaacagct ATGACCATGC AGCAATCGCA TCAGGCTGGT TACGCAAACG
      361 CCGCCGACCG GGAGTCTGGC ATCCCCGCAG CCGTACTCGA TGGCATCAAG GCCGTGGCGA
      421 AGGAAAAAAA CGCCACATTG ATGTTCCGCC TGGTCAACCC CCATTCCACC AGCCTGATTG
      481 CCGAAGGGGT GGCCACCAAA GGATTGGGCG TGCACGCCAA GTCGTCCGAT TGGGGGTTGC
      541 AGGCGGGCTA CATTCCCGTC AACCCGAATC TTTCCAAACT GTTCGGCCGT GCGCCCGAGG
      601 TGATCGCGCG GGCCGACAAC GACGTCAACA GCAGCCTGGC GCATGGCCAT ACCGCGGTCG
      661 ACCTGACGCT GTCGAAAGAG CGGCTTGACT ATCTGCGGCA AGCGGGCCTG GTCACCGGCA
      721 TGGCCGATGG CGTGGTCGCG AGCAACCACG CAGGCTACGA GCAGTTCGAG TTTCGCGTGA
      781 AGGAAACCTC GGACGGGCGC TATGCCGTGC AGTATCGCCG CAAGGGCGGC GACGATTTCG
      841 AGGCGGTCAA GGTGATCGGC AATGCCGCCG GTATTCCACT GACGGCGGAT ATCGACATGT
      901 TCGCCATTAT GCCGCATCTG TCCAACTTCC GCGACTCGGC GCGCAGTTCG GTGACCAGCG
      961 GCGATTCGGT GACCGATTAC CTGGCGCGCA CGCGGCGGGC TGCAGGGTCG ACTCTAGAGT
     1021 CAACCTTAAC CATGTCCGGT ATCGGCGGTA GTGAAAAATT CCGATGCCCT AATGCCCGGA
     1081 ACAGTGACGA TCCTTATTGT GAATCTATAA GCAGTAACTA TAAAGCATCT GTTGCAGGTG
     1141 TTTTAAAAGA CGGTATGAAA AGACAGGTTG GACAACCTTA CACTGATCAA AGTGTTCGCA
     1201 CATTAATGTT AACGCAAGCT TATTCCTCCG GTACGCCCGT TCGCTCGCAA AGTGAAATTG
     1261 CTCGCATTTG GGTCGCTCCC TATCTTGATA CGGACGGCGA TTTAAATGAC CAAAGTTTCA
     1321 CATATGTCGT TCTGAATCAG GGTGATTGGT TAATTGCCCA CAACGGGCAA CAGATTATAA
     1381 ATGAATATCG TCCCATCCGC TTATTGGGGG ATGGGGCCAA AACTTCAGGC AGTTCGGTTG
     1441 AGACTTCTCC AACCAATTCA AACAACACAA ATGACCGCAT CCCCGATGTT GGCGTGAATC
     1501 TTCATCTGCA GGAAACATCG GGTATTCCCG GTACCGTTCG ATAGGGATCC CCGGGTACCT
     1561 AAGTAActaa gaattcggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
     1621 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
     1681 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg cgctgatgtc
     1741 cggcggtgct tttgccgtta cgcaccaccc cgtcagtagc tgaacaggag ggacaggggg
     1801 tgggcgaaga actccagcat gagatccccg cgctggagga tcatccagcc ggcgtcccgg
     1861 aaaacgattc cgaagcccaa cctttcatag aaggcggcgg tggaatcgaa atctcgtgat
     1921 ggcaggttgg gcgtcgcttg gtcggtcatt tcgaacccca gagtcccgct cagaagaact
     1981 cgtcaagaag gcgatagaag gcgatgcgct gcgaatcggg agcggcgata ccgtaaagca
     2041 cgaggaagcg gtcagcccat tcgccgccaa gctcttcagc aatatcacgg gtagccaacg
     2101 ctatgtcctg atagcggtcc gccacaccca gccggccaca gtcgatgaat ccagaaaagc
     2161 ggccattttc caccatgata ttcggcaagc aggcatcgcc atgggtcacg acgagatcct
     2221 cgccgtcggg catccgcgcc ttgagcctgg cgaacagttc ggctggcgcg agcccctgat
     2281 gctcttcgtc cagatcatcc tgatcgacaa gaccggcttc catccgagta cgtgctcgct
     2341 cgatgcgatg tttcgcttgg tggtcgaatg ggcaggtagc cggatcaagc gtatgcagcc
     2401 gccgcattgc atcagccatg atggatactt tctcggcagg agcaaggtga gatgacagga
     2461 gatcctgccc cggcacttcg cccaatagca gccagtccct tcccgcttca gtgacaacgt
     2521 cgagcacagc tgcgcaagga acgcccgtcg tggccagcca cgatagccgc gctgcctcgt
     2581 cttggagttc attcagggca ccggacaggt cggtcttgac aaaaagaacc gggcgcccct
     2641 gcgctgacag ccggaacacg gcggcatcag agcagccgat tgtctgttgt gcccagtcat
     2701 agccgaatag cctctccacc caagcggccg gagaacctgc gtgcaatcca tcttgttcaa
     2761 tcatgcgaaa cgatcctcat cctgtctctt gatcagatct tgatcccctg cgccatcaga
     2821 tccttggcgg caagaaagcc atccagttta ctttgcaggg cttcccaacc ttaccagagg
     2881 gcgccccagc tggcaattcc ggttcgcttg ctgtccataa aaccgcccag tctagctatc
     2941 gccatgtaag cccactgcaa gctacctgct ttctctttgc gcttgcgttt tcccttgtcc
     3001 agatagccca gtagctgaca ttcatccggg gtcagcaccg tttctgcgga ctggctttct
     3061 acgtgttccg cttcctttag cagcccttgc gccctgagtg cttgcggcag cgtgaagctg
     3121 tgcctagaaa tattttatct gattaataag atgatcttct tgagatcgtt ttggtctgcg
     3181 cgtaatctct tgctctgaaa acgaaaaaac cgccttgcag ggcggttttt cgaaggttct
     3241 ctgagctacc aactctttga accgaggtaa ctggcttgga ggagcgcagt caccaaaact
     3301 tgtcctttca gtttagcctt aaccggcgca tgacttcaag actaactcct ctaaatcaat
     3361 taccagtggc tgctgccagt ggtgcttttg catgtctttc cgggttggac tcaagacgat
     3421 agttaccgga taaggcgcag cggtcggact gaacgggggg ttcgtgcata cagtccagct
     3481 tggagcgaac tgcctacccg gaactgagtg tcaggcgtgg aatgagacaa acgcggccat
     3541 aacagcggaa tgacaccggt aaaccgaaag gcaggaacag gagagcgcac gagggagccg
     3601 ccaggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccaccac tgatttgagc
     3661 gtcagatttc gtgatgcttg tcaggggggc ggagcctatg gaaaaacggc tttgccgcgg
     3721 ccctctcact tccctgttaa gtatcttcct ggcatcttcc aggaaatctc cgccccgttc
     3781 gtaagccatt tccgctcgcc gcagtcgaac gaccgagcgt agcgagtcag tgagcgagga
     3841 agcggaatat atcctgtatc acatattctg ctgacgcacc ggtgcagcct tttttctcct
     3901 gccacatgaa gcacttcact gacaccctca tcagtgccaa catagtaagc cagtatatac
     3961 actccgct
//