LOCUS       pKTM25TraVNG            4088 bp ds-DNA     circular     07-JUL-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_binding    281..303
                     /label="LacO"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    1839..1881
                     /label="Terminator"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    321..998
                     /label="TM25"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    1007..1129
                     /label="oppB fragment"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     CDS             1782..1850
                     /label="LacZ alpha"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    join(3180..4088,1..5)
                     /label="p15A"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     promoter        247..277
                     /label="lac promoter"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    1137..1661
                     /label="traV-NG"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    160..276
                     /label="Plac promoter"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    215..227
                     /label="CAP"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer_bind     complement(1694..1711)
                     /label="M13-fwd"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    complement(2093..2893)
                     /label="KanR"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
ORIGIN
        1 AAGCTTTAAT GCGGTAGTTT ATCACAGTTA AATTGCTAAC GCAGTCAGGC ACCGTGTATG
       61 AAATCTAACA ATGCGCTCAT CGTCATCCTC GGCACCGTCA CCCTGGATGC TGTAGGCATA
      121 GGCTTGGTTA TGCCGGTACT GCCGGGCCTC TTGCGGGATC TGGCACGACA GGTTTCCCGA
      181 CTGGAAAGCG GGCAGTGAGC GCAACGCAAT TAATGTGAGT TAGCTCACTC ATTAGGCACC
      241 CCAGGCTTTA CACTTTATGC TTCCGGCTCG TATGTTGTGT GGAATTGTGA GCGGATAACA
      301 ATTTCACACA GGAAACAGCT ATGACCATGC AGCAATCGCA TCAGGCTGGT TACGCAAACG
      361 CCGCCGACCG GGAGTCTGGC ATCCCCGCAG CCGTACTCGA TGGCATCAAG GCCGTGGCGA
      421 AGGAAAAAAA CGCCACATTG ATGTTCCGCC TGGTCAACCC CCATTCCACC AGCCTGATTG
      481 CCGAAGGGGT GGCCACCAAA GGATTGGGCG TGCACGCCAA GTCGTCCGAT TGGGGGTTGC
      541 AGGCGGGCTA CATTCCCGTC AACCCGAATC TTTCCAAACT GTTCGGCCGT GCGCCCGAGG
      601 TGATCGCGCG GGCCGACAAC GACGTCAACA GCAGCCTGGC GCATGGCCAT ACCGCGGTCG
      661 ACCTGACGCT GTCGAAAGAG CGGCTTGACT ATCTGCGGCA AGCGGGCCTG GTCACCGGCA
      721 TGGCCGATGG CGTGGTCGCG AGCAACCACG CAGGCTACGA GCAGTTCGAG TTTCGCGTGA
      781 AGGAAACCTC GGACGGGCGC TATGCCGTGC AGTATCGCCG CAAGGGCGGC GACGATTTCG
      841 AGGCGGTCAA GGTGATCGGC AATGCCGCCG GTATTCCACT GACGGCGGAT ATCGACATGT
      901 TCGCCATTAT GCCGCATCTG TCCAACTTCC GCGACTCGGC GCGCAGTTCG GTGACCAGCG
      961 GCGATTCGGT GACCGATTAC CTGGCGCGCA CGCGGCGGGC TGCAGGGTTA AAATTTATTC
     1021 TACGTCGCTG TCTGGAAGCG ATTCCGACGC TATTTATTCT TATTACTATT TCGTTCTTTA
     1081 TGATGCGCCT CGCGCCGGGA AGCCCTTTTA CCGGCGAACG TACTTTACCT CTAGAGTCAA
     1141 CCTTAACCAT GTCCGGTATC GGCGGTAGTG AAAAATTCCG ATGCCCTAAT GCCCGGAACA
     1201 GTGACGATCC TTATTGTGAA TCTATAAGCA GTAACTATAA AGCATCTGTT GCAGGTGTTT
     1261 TAAAAGACGG TATGAAAAGA CAGGTTGGAC AACCTTACAC TGATCAAAGT GTTCGCACAT
     1321 TAATGTTAAC GCAAGCTTAT TCCTCCGGTA CGCCCGTTCG CTCGCAAAGT GAAATTGCTC
     1381 GCATTTGGGT CGCTCCCTAT CTTGATACGG ACGGCGATTT AAATGACCAA AGTTTCACAT
     1441 ATGTCGTTCT GAATCAGGGT GATTGGTTAA TTGCCCACAA CGGGCAACAG ATTATAAATG
     1501 AATATCGTCC CATCCGCTTA TTGGGGGATG GGGCCAAAAC TTCAGGCAGT TCGGTTGAGA
     1561 CTTCTCCAAC CAATTCAAAC AACACAAATG ACCGCATCCC CGATGTTGGC GTGAATCTTC
     1621 ATCTGCAGGA AACATCGGGT ATTCCCGGTA CCGTTCGATA GGGATCCCCG GGTACCTAAG
     1681 TAAGTAAGAA TTCACTGGCC GTCGTTTTAC AACGTCGTGA CTGGGAAAAC CCTGGCGTTA
     1741 CCCAACTTAA TCGCCTTGCA GCACATCCCC CTTTCGCCAG CTGGCGTAAT AGCGAAGAGG
     1801 CCCGCACCGA TCGCCCTTCC CAACAGTTGC GCAGCCTGAA TGGCGAATGG CGCTGATGTC
     1861 CGGCGGTGCT TTTGCCGTTA CGCACCACCC CGTCAGTAGC TGAACAGGAG GGACAGGGGG
     1921 TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG
     1981 AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT
     2041 GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT
     2101 CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA
     2161 CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG
     2221 CTATGTCCTG ATAGCGGTCC GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC
     2281 GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT
     2341 CGCCGTCGGG CATCCGCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT
     2401 GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT
     2461 CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC
     2521 GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA
     2581 GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT
     2641 CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT
     2701 CTTGGAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT
     2761 GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT
     2821 AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA
     2881 TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCAGATCT TGATCCCCTG CGCCATCAGA
     2941 TCCTTGGCGG CAAGAAAGCC ATCCAGTTTA CTTTGCAGGG CTTCCCAACC TTACCAGAGG
     3001 GCGCCCCAGC TGGCAATTCC GGTTCGCTTG CTGTCCATAA AACCGCCCAG TCTAGCTATC
     3061 GCCATGTAAG CCCACTGCAA GCTACCTGCT TTCTCTTTGC GCTTGCGTTT TCCCTTGTCC
     3121 AGATAGCCCA GTAGCTGACA TTCATCCGGG GTCAGCACCG TTTCTGCGGA CTGGCTTTCT
     3181 ACGTGTTCCG CTTCCTTTAG CAGCCCTTGC GCCCTGAGTG CTTGCGGCAG CGTGAAGCTG
     3241 TGCCTAGAAA TATTTTATCT GATTAATAAG ATGATCTTCT TGAGATCGTT TTGGTCTGCG
     3301 CGTAATCTCT TGCTCTGAAA ACGAAAAAAC CGCCTTGCAG GGCGGTTTTT CGAAGGTTCT
     3361 CTGAGCTACC AACTCTTTGA ACCGAGGTAA CTGGCTTGGA GGAGCGCAGT CACCAAAACT
     3421 TGTCCTTTCA GTTTAGCCTT AACCGGCGCA TGACTTCAAG ACTAACTCCT CTAAATCAAT
     3481 TACCAGTGGC TGCTGCCAGT GGTGCTTTTG CATGTCTTTC CGGGTTGGAC TCAAGACGAT
     3541 AGTTACCGGA TAAGGCGCAG CGGTCGGACT GAACGGGGGG TTCGTGCATA CAGTCCAGCT
     3601 TGGAGCGAAC TGCCTACCCG GAACTGAGTG TCAGGCGTGG AATGAGACAA ACGCGGCCAT
     3661 AACAGCGGAA TGACACCGGT AAACCGAAAG GCAGGAACAG GAGAGCGCAC GAGGGAGCCG
     3721 CCAGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCAC TGATTTGAGC
     3781 GTCAGATTTC GTGATGCTTG TCAGGGGGGC GGAGCCTATG GAAAAACGGC TTTGCCGCGG
     3841 CCCTCTCACT TCCCTGTTAA GTATCTTCCT GGCATCTTCC AGGAAATCTC CGCCCCGTTC
     3901 GTAAGCCATT TCCGCTCGCC GCAGTCGAAC GACCGAGCGT AGCGAGTCAG TGAGCGAGGA
     3961 AGCGGAATAT ATCCTGTATC ACATATTCTG CTGACGCACC GGTGCAGCCT TTTTTCTCCT
     4021 GCCACATGAA GCACTTCACT GACACCCTCA TCAGTGCCAA CATAGTAAGC CAGTATATAC
     4081 ACTCCGCT
//