LOCUS       pKT25TraVF_(OK)         3873 bp ds-DNA     circular     09-JUL-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    1624..1666
                     /label="Terminator"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    1026..1475
                     /label="traV-Fp"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    321..998
                     /label="T25"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_binding    281..303
                     /label="LacO"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    complement(1878..2678)
                     /label="KanR"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    join(2965..3873,1..5)
                     /label="p15A"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             1567..1635
                     /label="LacZ alpha"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    215..227
                     /label="CAP"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     promoter        247..277
                     /label="lac promoter"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    160..276
                     /label="Plac promoter"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
ORIGIN
        1 aagctttaat gcggtagttt atcacagtta aattgctaac gcagtcaggc accgtgtatg
       61 aaatctaaca atgcgctcat cgtcatcctc ggcaccgtca ccctggatgc tgtaggcata
      121 ggcttggtta tgccggtact gccgggcctc ttgcgggatc tggcacgaca ggtttcccga
      181 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
      241 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
      301 atttcacaca ggaaacagct ATGACCATGC AGCAATCGCA TCAGGCTGGT TACGCAAACG
      361 CCGCCGACCG GGAGTCTGGC ATCCCCGCAG CCGTACTCGA TGGCATCAAG GCCGTGGCGA
      421 AGGAAAAAAA CGCCACATTG ATGTTCCGCC TGGTCAACCC CCATTCCACC AGCCTGATTG
      481 CCGAAGGGGT GGCCACCAAA GGATTGGGCG TGCACGCCAA GTCGTCCGAT TGGGGGTTGC
      541 AGGCGGGCTA CATTCCCGTC AACCCGAATC TTTCCAAACT GTTCGGCCGT GCGCCCGAGG
      601 TGATCGCGCG GGCCGACAAC GACGTCAACA GCAGCCTGGC GCATGGCCAT ACCGCGGTCG
      661 ACCTGACGCT GTCGAAAGAG CGGCTTGACT ATCTGCGGCA AGCGGGCCTG GTCACCGGCA
      721 TGGCCGATGG CGTGGTCGCG AGCAACCACG CAGGCTACGA GCAGTTCGAG TTTCGCGTGA
      781 AGGAAACCTC GGACGGGCGC TATGCCGTGC AGTATCGCCG CAAGGGCGGC GACGATTTCG
      841 AGGCGGTCAA GGTGATCGGC AATGCCGCCG GTATTCCACT GACGGCGGAT ATCGACATGT
      901 TCGCCATTAT GCCGCATCTG TCCAACTTCC GCGACTCGGC GCGCAGTTCG GTGACCAGCG
      961 GCGATTCGGT GACCGATTAC CTGGCGCGCA CGCGGCGGGC TGCAGGGTCG ACTCTAGAGG
     1021 ATCCCAGTAC GGAATTTGAG TGTAACGCCA CCACATCCGA TACCTGTATG ACGATGGAGC
     1081 AGGCCAATGA GAAGGCCAAA AAACTGGAGC GGTCCTCAGA AGCAAAGCCG GTTGCGGCAT
     1141 CACTGCCGCG CCTGGCTGAA GGGAACTTCC GGACAATGCC GGTGCAGACC GTCACCGCGA
     1201 CCACTCCGTC CGGCAGCAGA CCTGCGGTGA CAGCACATCC GGAGCAGAAA CTGCTGGCAC
     1261 CTCGCCCGCT GTTTACCGCT GCCCGGGAAG TGAAAACGGT TGTTCCGGTC AGTTCAGTTA
     1321 CGCCGGTAAC ACCTCCTCGT CCGCTAAGAA CGGGTGAGCA GACGGCAGCA TTATGGATAG
     1381 CGCCTTATAT TGATAACCAG GATGTTTATC ATCAGCCATC CAGCGTGTTT TTTGTTATTA
     1441 AACCGTCTGC GTGGGGAAAA CCACGTATTA ATTAAgaatt cggccgtcgt tttacaacgt
     1501 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     1561 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     1621 ctgaatggcg aatggcgctg atgtccggcg gtgcttttgc cgttacgcac caccccgtca
     1681 gtagctgaac aggagggaca gggggtgggc gaagaactcc agcatgagat ccccgcgctg
     1741 gaggatcatc cagccggcgt cccggaaaac gattccgaag cccaaccttt catagaaggc
     1801 ggcggtggaa tcgaaatctc gtgatggcag gttgggcgtc gcttggtcgg tcatttcgaa
     1861 ccccagagtc ccgctcagaa gaactcgtca agaaggcgat agaaggcgat gcgctgcgaa
     1921 tcgggagcgg cgataccgta aagcacgagg aagcggtcag cccattcgcc gccaagctct
     1981 tcagcaatat cacgggtagc caacgctatg tcctgatagc ggtccgccac acccagccgg
     2041 ccacagtcga tgaatccaga aaagcggcca ttttccacca tgatattcgg caagcaggca
     2101 tcgccatggg tcacgacgag atcctcgccg tcgggcatcc gcgccttgag cctggcgaac
     2161 agttcggctg gcgcgagccc ctgatgctct tcgtccagat catcctgatc gacaagaccg
     2221 gcttccatcc gagtacgtgc tcgctcgatg cgatgtttcg cttggtggtc gaatgggcag
     2281 gtagccggat caagcgtatg cagccgccgc attgcatcag ccatgatgga tactttctcg
     2341 gcaggagcaa ggtgagatga caggagatcc tgccccggca cttcgcccaa tagcagccag
     2401 tcccttcccg cttcagtgac aacgtcgagc acagctgcgc aaggaacgcc cgtcgtggcc
     2461 agccacgata gccgcgctgc ctcgtcttgg agttcattca gggcaccgga caggtcggtc
     2521 ttgacaaaaa gaaccgggcg cccctgcgct gacagccgga acacggcggc atcagagcag
     2581 ccgattgtct gttgtgccca gtcatagccg aatagcctct ccacccaagc ggccggagaa
     2641 cctgcgtgca atccatcttg ttcaatcatg cgaaacgatc ctcatcctgt ctcttgatca
     2701 gatcttgatc ccctgcgcca tcagatcctt ggcggcaaga aagccatcca gtttactttg
     2761 cagggcttcc caaccttacc agagggcgcc ccagctggca attccggttc gcttgctgtc
     2821 cataaaaccg cccagtctag ctatcgccat gtaagcccac tgcaagctac ctgctttctc
     2881 tttgcgcttg cgttttccct tgtccagata gcccagtagc tgacattcat ccggggtcag
     2941 caccgtttct gcggactggc tttctacgtg ttccgcttcc tttagcagcc cttgcgccct
     3001 gagtgcttgc ggcagcgtga agctgtgcct agaaatattt tatctgatta ataagatgat
     3061 cttcttgaga tcgttttggt ctgcgcgtaa tctcttgctc tgaaaacgaa aaaaccgcct
     3121 tgcagggcgg tttttcgaag gttctctgag ctaccaactc tttgaaccga ggtaactggc
     3181 ttggaggagc gcagtcacca aaacttgtcc tttcagttta gccttaaccg gcgcatgact
     3241 tcaagactaa ctcctctaaa tcaattacca gtggctgctg ccagtggtgc ttttgcatgt
     3301 ctttccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc ggactgaacg
     3361 gggggttcgt gcatacagtc cagcttggag cgaactgcct acccggaact gagtgtcagg
     3421 cgtggaatga gacaaacgcg gccataacag cggaatgaca ccggtaaacc gaaaggcagg
     3481 aacaggagag cgcacgaggg agccgccagg ggaaacgcct ggtatcttta tagtcctgtc
     3541 gggtttcgcc accactgatt tgagcgtcag atttcgtgat gcttgtcagg ggggcggagc
     3601 ctatggaaaa acggctttgc cgcggccctc tcacttccct gttaagtatc ttcctggcat
     3661 cttccaggaa atctccgccc cgttcgtaag ccatttccgc tcgccgcagt cgaacgaccg
     3721 agcgtagcga gtcagtgagc gaggaagcgg aatatatcct gtatcacata ttctgctgac
     3781 gcaccggtgc agcctttttt ctcctgccac atgaagcact tcactgacac cctcatcagt
     3841 gccaacatag taagccagta tatacactcc gct
//