LOCUS       pET22bTraVF_(OK)        5886 bp ds-DNA     circular     09-JUL-2020
DEFINITION  .
SOURCE      synthetic DNA construct                                            
  ORGANISM  synthetic DNA construct                                            
FEATURES             Location/Qualifiers
     primer_bind     join(5877..5886,1..18)
                     /label="primer_bind"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer_bind     5430..5460
                     /label="primer_bind"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     promoter        692..720
                     /label="amp prom"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     gene            5440..5886
                     /label="traV-Fp"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_binding    5299..5321
                     /label="LacO"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     rep_origin      175..630
                     /label="M13 origin"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     CDS             960..1619
                     /label="AmpR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    5368..5439
                     /label="pelB leader"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer_bind     complement(5422..5449)
                     /label="primer_bind"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer_bind     5280..5299
                     /label="T7"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer_bind     complement(join(5868..5886,1..10))
                     /label="primer_bind"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     CDS             7..24
                     /label="6x His Tag"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     rep_origin      192..498
                     /label="F1 ori"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     rep_origin      1717..2399
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
ORIGIN
        1 ctcgagcacc accaccacca ccactgagat ccggctgcta acaaagcccg aaaggaagct
       61 gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg
      121 gtcttgaggg gttttttgct gaaaggagga actatatccg gattggcgaa tgggacgcgc
      181 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
      241 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
      301 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
      361 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
      421 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
      481 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
      541 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
      601 attttaacaa aatattaacg tttacaattt caggtggcac ttttcgggga aatgtgcgcg
      661 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
      721 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
      781 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
      841 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
      901 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
      961 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     1021 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     1081 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     1141 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     1201 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     1261 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa
     1321 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     1381 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     1441 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     1501 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     1561 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     1621 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     1681 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     1741 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     1801 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     1861 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     1921 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
     1981 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     2041 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     2101 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     2161 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     2221 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     2281 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     2341 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     2401 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     2461 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     2521 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt
     2581 ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat
     2641 ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc
     2701 atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
     2761 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
     2821 tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga
     2881 agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc
     2941 gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc
     3001 actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga
     3061 gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt
     3121 gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa
     3181 tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg
     3241 atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa
     3301 acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag
     3361 tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc
     3421 cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ggccgccatg
     3481 ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct
     3541 tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc
     3601 cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt
     3661 tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg
     3721 aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gagatcccgg tgcctaatga
     3781 gtgagctaac ttacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     3841 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     3901 cgccagggtg gtttttcttt tcaccagtga gacgggcaac agctgattgc ccttcaccgc
     3961 ctggccctga gagagttgca gcaagcggtc cacgctggtt tgccccagca ggcgaaaatc
     4021 ctgtttgatg gtggttaacg gcgggatata acatgagctg tcttcggtat cgtcgtatcc
     4081 cactaccgag atatccgcac caacgcgcag cccggactcg gtaatggcgc gcattgcgcc
     4141 cagcgccatc tgatcgttgg caaccagcat cgcagtggga acgatgccct cattcagcat
     4201 ttgcatggtt tgttgaaaac cggacatggc actccagtcg ccttcccgtt ccgctatcgg
     4261 ctgaatttga ttgcgagtga gatatttatg ccagccagcc agacgcagac gcgccgagac
     4321 agaacttaat gggcccgcta acagcgcgat ttgctggtga cccaatgcga ccagatgctc
     4381 cacgcccagt cgcgtaccgt cttcatggga gaaaataata ctgttgatgg gtgtctggtc
     4441 agagacatca agaaataacg ccggaacatt agtgcaggca gcttccacag caatggcatc
     4501 ctggtcatcc agcggatagt taatgatcag cccactgacg cgttgcgcga gaagattgtg
     4561 caccgccgct ttacaggctt cgacgccgct tcgttctacc atcgacacca ccacgctggc
     4621 acccagttga tcggcgcgag atttaatcgc cgcgacaatt tgcgacggcg cgtgcagggc
     4681 cagactggag gtggcaacgc caatcagcaa cgactgtttg cccgccagtt gttgtgccac
     4741 gcggttggga atgtaattca gctccgccat cgccgcttcc actttttccc gcgttttcgc
     4801 agaaacgtgg ctggcctggt tcaccacgcg ggaaacggtc tgataagaga caccggcata
     4861 ctctgcgaca tcgtataacg ttactggttt cacattcacc accctgaatt gactctcttc
     4921 cgggcgctat catgccatac cgcgaaaggt tttgcgccat tcgatggtgt ccgggatctc
     4981 gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt
     5041 tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg
     5101 ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag
     5161 cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc
     5221 cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaatt
     5281 aatacgactc actatagggg aattgtgagc ggataacaat tcccctctag aaataatttt
     5341 gtttaacttt aagaaggaga tatacatatg aaatacctgc tgccgaccgc tgctgctggt
     5401 ctgctgctcc tcgctgccca gccggcgatg gccatggata gtacggaatt tgagtgtaac
     5461 gccaccacat ccgatacctg tatgacgatg gagcaggcca atgagaaggc caaaaaactg
     5521 gagcggtcct cagaagcaaa gccggttgcg gcatcactgc cgcgcctggc tgaagggaac
     5581 ttccggacaa tgccggtgca gaccgtcacc gcgaccactc cgtccggcag cagacctgcg
     5641 gtgacagcac atccggagca gaaactgctg gcacctcgcc cgctgtttac cgctgcccgg
     5701 gaagtgaaaa cggttgttcc ggtcagttca gttacgccgg taacacctcc tcgtccgcta
     5761 agaacgggtg agcagacggc agcattatgg atagcgcctt atattgataa ccaggatgtt
     5821 tatcatcagc catccagcgt gttttttgtt attaaaccgt ctgcgtgggg aaaaccacgt
     5881 attaat
//