LOCUS       pKTM25TraVFp            3993 bp ds-DNA     circular     07-JUL-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     primer_bind     complement(1599..1616)
                     /label="M13-fwd"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    1143..1592
                     /label="traV-Fp"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     promoter        247..277
                     /label="lac promoter"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    1744..1786
                     /label="Terminator"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    complement(1998..2798)
                     /label="KanR"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             1687..1755
                     /label="LacZ alpha"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    215..227
                     /label="CAP"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    join(3085..3993,1..5)
                     /label="p15A"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_binding    281..303
                     /label="LacO"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    1007..1129
                     /label="oppB fragment"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    160..276
                     /label="Plac promoter"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    321..998
                     /label="TM25"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
ORIGIN
        1 AAGCTTTAAT GCGGTAGTTT ATCACAGTTA AATTGCTAAC GCAGTCAGGC ACCGTGTATG
       61 AAATCTAACA ATGCGCTCAT CGTCATCCTC GGCACCGTCA CCCTGGATGC TGTAGGCATA
      121 GGCTTGGTTA TGCCGGTACT GCCGGGCCTC TTGCGGGATC TGGCACGACA GGTTTCCCGA
      181 CTGGAAAGCG GGCAGTGAGC GCAACGCAAT TAATGTGAGT TAGCTCACTC ATTAGGCACC
      241 CCAGGCTTTA CACTTTATGC TTCCGGCTCG TATGTTGTGT GGAATTGTGA GCGGATAACA
      301 ATTTCACACA GGAAACAGCT ATGACCATGC AGCAATCGCA TCAGGCTGGT TACGCAAACG
      361 CCGCCGACCG GGAGTCTGGC ATCCCCGCAG CCGTACTCGA TGGCATCAAG GCCGTGGCGA
      421 AGGAAAAAAA CGCCACATTG ATGTTCCGCC TGGTCAACCC CCATTCCACC AGCCTGATTG
      481 CCGAAGGGGT GGCCACCAAA GGATTGGGCG TGCACGCCAA GTCGTCCGAT TGGGGGTTGC
      541 AGGCGGGCTA CATTCCCGTC AACCCGAATC TTTCCAAACT GTTCGGCCGT GCGCCCGAGG
      601 TGATCGCGCG GGCCGACAAC GACGTCAACA GCAGCCTGGC GCATGGCCAT ACCGCGGTCG
      661 ACCTGACGCT GTCGAAAGAG CGGCTTGACT ATCTGCGGCA AGCGGGCCTG GTCACCGGCA
      721 TGGCCGATGG CGTGGTCGCG AGCAACCACG CAGGCTACGA GCAGTTCGAG TTTCGCGTGA
      781 AGGAAACCTC GGACGGGCGC TATGCCGTGC AGTATCGCCG CAAGGGCGGC GACGATTTCG
      841 AGGCGGTCAA GGTGATCGGC AATGCCGCCG GTATTCCACT GACGGCGGAT ATCGACATGT
      901 TCGCCATTAT GCCGCATCTG TCCAACTTCC GCGACTCGGC GCGCAGTTCG GTGACCAGCG
      961 GCGATTCGGT GACCGATTAC CTGGCGCGCA CGCGGCGGGC TGCAGGGTTA AAATTTATTC
     1021 TACGTCGCTG TCTGGAAGCG ATTCCGACGC TATTTATTCT TATTACTATT TCGTTCTTTA
     1081 TGATGCGCCT CGCGCCGGGA AGCCCTTTTA CCGGCGAACG TACTTTACCT CTAGAGGATC
     1141 CCAGTACGGA ATTTGAGTGT AACGCCACCA CATCCGATAC CTGTATGACG ATGGAGCAGG
     1201 CCAATGAGAA GGCCAAAAAA CTGGAGCGGT CCTCAGAAGC AAAGCCGGTT GCGGCATCAC
     1261 TGCCGCGCCT GGCTGAAGGG AACTTCCGGA CAATGCCGGT GCAGACCGTC ACCGCGACCA
     1321 CTCCGTCCGG CAGCAGACCT GCGGTGACAG CACATCCGGA GCAGAAACTG CTGGCACCTC
     1381 GCCCGCTGTT TACCGCTGCC CGGGAAGTGA AAACGGTTGT TCCGGTCAGT TCAGTTACGC
     1441 CGGTAACACC TCCTCGTCCG CTAAGAACGG GTGAGCAGAC GGCAGCATTA TGGATAGCGC
     1501 CTTATATTGA TAACCAGGAT GTTTATCATC AGCCATCCAG CGTGTTTTTT GTTATTAAAC
     1561 CGTCTGCGTG GGGAAAACCA CGTATTAATT AAGAATTCAC TGGCCGTCGT TTTACAACGT
     1621 CGTGACTGGG AAAACCCTGG CGTTACCCAA CTTAATCGCC TTGCAGCACA TCCCCCTTTC
     1681 GCCAGCTGGC GTAATAGCGA AGAGGCCCGC ACCGATCGCC CTTCCCAACA GTTGCGCAGC
     1741 CTGAATGGCG AATGGCGCTG ATGTCCGGCG GTGCTTTTGC CGTTACGCAC CACCCCGTCA
     1801 GTAGCTGAAC AGGAGGGACA GGGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG
     1861 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC
     1921 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA
     1981 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA
     2041 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT
     2101 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GGTCCGCCAC ACCCAGCCGG
     2161 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA
     2221 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATCC GCGCCTTGAG CCTGGCGAAC
     2281 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG
     2341 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG
     2401 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG
     2461 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG
     2521 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC
     2581 AGCCACGATA GCCGCGCTGC CTCGTCTTGG AGTTCATTCA GGGCACCGGA CAGGTCGGTC
     2641 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG
     2701 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA
     2761 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCA
     2821 GATCTTGATC CCCTGCGCCA TCAGATCCTT GGCGGCAAGA AAGCCATCCA GTTTACTTTG
     2881 CAGGGCTTCC CAACCTTACC AGAGGGCGCC CCAGCTGGCA ATTCCGGTTC GCTTGCTGTC
     2941 CATAAAACCG CCCAGTCTAG CTATCGCCAT GTAAGCCCAC TGCAAGCTAC CTGCTTTCTC
     3001 TTTGCGCTTG CGTTTTCCCT TGTCCAGATA GCCCAGTAGC TGACATTCAT CCGGGGTCAG
     3061 CACCGTTTCT GCGGACTGGC TTTCTACGTG TTCCGCTTCC TTTAGCAGCC CTTGCGCCCT
     3121 GAGTGCTTGC GGCAGCGTGA AGCTGTGCCT AGAAATATTT TATCTGATTA ATAAGATGAT
     3181 CTTCTTGAGA TCGTTTTGGT CTGCGCGTAA TCTCTTGCTC TGAAAACGAA AAAACCGCCT
     3241 TGCAGGGCGG TTTTTCGAAG GTTCTCTGAG CTACCAACTC TTTGAACCGA GGTAACTGGC
     3301 TTGGAGGAGC GCAGTCACCA AAACTTGTCC TTTCAGTTTA GCCTTAACCG GCGCATGACT
     3361 TCAAGACTAA CTCCTCTAAA TCAATTACCA GTGGCTGCTG CCAGTGGTGC TTTTGCATGT
     3421 CTTTCCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGACTGAACG
     3481 GGGGGTTCGT GCATACAGTC CAGCTTGGAG CGAACTGCCT ACCCGGAACT GAGTGTCAGG
     3541 CGTGGAATGA GACAAACGCG GCCATAACAG CGGAATGACA CCGGTAAACC GAAAGGCAGG
     3601 AACAGGAGAG CGCACGAGGG AGCCGCCAGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC
     3661 GGGTTTCGCC ACCACTGATT TGAGCGTCAG ATTTCGTGAT GCTTGTCAGG GGGGCGGAGC
     3721 CTATGGAAAA ACGGCTTTGC CGCGGCCCTC TCACTTCCCT GTTAAGTATC TTCCTGGCAT
     3781 CTTCCAGGAA ATCTCCGCCC CGTTCGTAAG CCATTTCCGC TCGCCGCAGT CGAACGACCG
     3841 AGCGTAGCGA GTCAGTGAGC GAGGAAGCGG AATATATCCT GTATCACATA TTCTGCTGAC
     3901 GCACCGGTGC AGCCTTTTTT CTCCTGCCAC ATGAAGCACT TCACTGACAC CCTCATCAGT
     3961 GCCAACATAG TAAGCCAGTA TATACACTCC GCT
//