LOCUS       pUTM18CTraVF            3576 bp ds-DNA     circular     07-JUL-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_binding    125..147
                     /label="LacO"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    4..120
                     /label="Plac promoter"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     marker          1724..2584
                     /label="amp marker"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     rep_origin      2679..3361
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     promoter        91..121
                     /label="lac promoter"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    722..844
                     /label="oppB fragment"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    864..1313
                     /label="traV-Fp"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer_bind     153..173
                     /label="M13-rev"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    165..713
                     /label="T18"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    59..71
                     /label="CAP"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     CDS             1922..2581
                     /label="AmpR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     promoter        1654..1682
                     /label="amp prom"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
ORIGIN
        1 CAGCTGGCAC GACAGGTTTC CCGACTGGAA AGCGGGCAGT GAGCGCAACG CAATTAATGT
       61 GAGTTAGCTC ACTCATTAGG CACCCCAGGC TTTACACTTT ATGCTTCCGG CTCGTATGTT
      121 GTGTGGAATT GTGAGCGGAT AACAATTTCA CACAGGAAAC AGCTATGACC ATGATTACGC
      181 CAAGCTTAGC CGCCAGCGAG GCCACGGGCG GCCTGGATCG CGAACGCATC GACTTGTTGT
      241 GGAAAATCGC TCGCGCCGGC GCCCGTTCCG CAGTGGGCAC CGAGGCGCGT CGCCAGTTCC
      301 GCTACGACGG CGACATGAAT ATCGGCGTGA TCACCGATTT CGAGCTGGAA GTGCGCAATG
      361 CGCTGAACAG GCGGGCGCAC GCCGTCGGCG CGCAGGACGT GGTCCAGCAT GGCACTGAGC
      421 AGAACAATCC TTTCCCGGAG GCAGATGAGA AGATTTTCGT CGTATCGGCC ACCGGTGAAA
      481 GCCAGATGCT CACGCGCGGG CAACTGAAGG AATACATTGG CCAGCAGCGC GGCGAGGGCT
      541 ATGTCTTCTA CGAGAACCGT GCATACGGCG TGGCGGGGAA AAGCCTGTTC GACGATGGGC
      601 TGGGAGCCGC GCCCGGCGTG CCGAGCGGAC GTTCGAAGTT CTCGCCGGAT GTACTGGAAA
      661 CGGTGCCGGC GTCACCCGGA TTGCGGCGGC CGTCGCTGGG CGCAGTGGAA CGCCACTGCA
      721 GGTTAAAATT TATTCTACGT CGCTGTCTGG AAGCGATTCC GACGCTATTT ATTCTTATTA
      781 CTATTTCGTT CTTTATGATG CGCCTCGCGC CGGGAAGCCC TTTTACCGGC GAACGTACTT
      841 TACCGTCGAC TCTAGAGGAT CCCAGTACGG AATTTGAGTG TAACGCCACC ACATCCGATA
      901 CCTGTATGAC GATGGAGCAG GCCAATGAGA AGGCCAAAAA ACTGGAGCGG TCCTCAGAAG
      961 CAAAGCCGGT TGCGGCATCA CTGCCGCGCC TGGCTGAAGG GAACTTCCGG ACAATGCCGG
     1021 TGCAGACCGT CACCGCGACC ACTCCGTCCG GCAGCAGACC TGCGGTGACA GCACATCCGG
     1081 AGCAGAAACT GCTGGCACCT CGCCCGCTGT TTACCGCTGC CCGGGAAGTG AAAACGGTTG
     1141 TTCCGGTCAG TTCAGTTACG CCGGTAACAC CTCCTCGTCC GCTAAGAACG GGTGAGCAGA
     1201 CGGCAGCATT ATGGATAGCG CCTTATATTG ATAACCAGGA TGTTTATCAT CAGCCATCCA
     1261 GCGTGTTTTT TGTTATTAAA CCGTCTGCGT GGGGAAAACC ACGTATTAAT TAAGAATTCA
     1321 TCGATATAAC TAAGTAATAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT
     1381 AAGCCAGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC
     1441 GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC
     1501 ACCGTCATCA CCGAAACGCG CGAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT
     1561 TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG
     1621 CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA
     1681 ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT
     1741 CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA
     1801 AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA
     1861 ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT
     1921 GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTATTG ACGCCGGGCA
     1981 AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT
     2041 CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC
     2101 CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT
     2161 AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA
     2221 GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGTAG CAATGGCAAC
     2281 AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT
     2341 AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG
     2401 CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC
     2461 ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC
     2521 AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG
     2581 GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA
     2641 ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG
     2701 TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA
     2761 TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT
     2821 GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG
     2881 AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA
     2941 CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG
     3001 TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA
     3061 GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC
     3121 CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA
     3181 GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC
     3241 AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG
     3301 TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC
     3361 CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC
     3421 CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG
     3481 CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC CAATACGCAA
     3541 ACCGCCTCTC CCCGCGCGTT GGCCGATTCA TTAATG
//