LOCUS       pSA-CRISPR_BC          12586 bp ds-DNA     circular     15-NOV-2019
DEFINITION  .
SOURCE      synthetic DNA construct                                            
  ORGANISM  synthetic DNA construct                                            
FEATURES             Location/Qualifiers
     CDS             11000..11800
                     /label="ApmR"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    9104..9226
                     /label="BC-4"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     CDS             complement(153..1595)
                     /label="pSG5 Rep"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     promoter        3093..3394
                     /label="rpsL promoter"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    8138..9078
                     /label="LH"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     terminator      8103..8137
                     /label="lambda t0 terminator"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     oriT            2762..2871
                     /label="incP origin of transfer"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_RNA        8006..8081
                     /label="gRNA scaffold"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     rep_origin      complement(10208..10796)
                     /label="ori"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    9227..10170
                     /label="RH"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    9079..9103
                     /label="Universal primer"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    7986..8005
                     /label="Sa-gRNA1"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     CDS             3395..7501
                     /label="Cas9"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer_bind     complement(10288..10307)
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     promoter        7701..7985
                     /label="gapdh promoter"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          8113..8156
                     /label="BC305"
                     /note="sequence: tcggttgccgccgggcgttttttatGACGGGCTGGAGATCCTGC"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer          complement(10152..10195)
                     /label="BC308"
                     /note="sequence: gcggcctttttacggttcctggcctGACGACGTTGACGAGGAGC"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
ORIGIN
        1 cggacgctcg gactgggcgc gggattccag cagtaaccca ggtccgccgg ccacctcacg
       61 gcaggcagac cctcggcttt cgcgccggga ccgccatgag accgccaccc ggatgtccgg
      121 ggtggcggtc tcatggcggt ccctcagcgg ccctacgcga ccgctgtgtc gacgcggagg
      181 cagtctccgg ggtgctggcc ccagggctgg agtaccgggg cgagcttgcc cttgcagcgg
      241 cggcacacgg gcgacgcggc gccgggcggg ggagtcttga cgtcgacggt gaccggcttc
      301 ggcttcaggc gcttccgaag gctgatcgtc gggcggatcg cctccttctt cggctgacgc
      361 actcggtcga gcgaagcggc cagctcctcc gtgcgggcct cgttggcctt ccggcgcgcc
      421 tcgcggaaag cagcttcctc ctcggacatg acctcgaacc tcatctggtc agcgtcaagg
      481 tcgcccggcg cggccggcgc ttccggggcg ggcgggtcca ggacgtcctt gccccacacc
      541 aggccccagg actcgacgag ccgccggacg cccggtaggc cgtacgtctc ggcgaccttg
      601 atgaggtcga ggcgacgtcc ggcgacgcgg gcgatgtatc ggtaccagat gtaggccggg
      661 atgaccgcga tggcgaccag gccctcggtg tcgtcggtga tctcctcctc ggtgcggacg
      721 tcctgctgga tgccgagttc cttgatcagc cggttcaggt tctgcgaccg gtagtgcttg
      781 cggacctgga acacgccgaa ctcgcgctcg cggtacttct cgacgaacgg gccgggccga
      841 cgaagccgct gcagctcggc ggccgccgcg tcgcccaggt cgagcggtcc catgcggtcg
      901 tcgccgcgac cggccttgaa gttctgtccg gccagctcca ggccgatctt ggcgacgccg
      961 cccttggtct tgtcgccgtc cttgtagagg tagcgggcct gcttgcccgc atcgccgtca
     1021 gcggcgtccg cgccgttgag tgggcgcacg tcggtgccgt ggcccttgcc ctcacaggag
     1081 caaccgggcc ggtcgcacgt ctcgctgacg gtgtagccgc ccgcggattc gaccccggcg
     1141 gcccaggctc cggcgagtgc gtcgcggaac gcggcctggg cgtccgggcc gagcacctcg
     1201 cgggtgaccc agagcgtgtg ccagtgcagg tgccagccgg agccccagcc gaaggtgtcc
     1261 tcgaaggccc gctcgtagcc gatgatcccg aagtcgtcgc gcatcgtgcg ccagcggcgg
     1321 ccggacgagc cgtacgcgcc cttccagccg tcgtgcaaga ccgcgaccag gccgtgccgc
     1381 attcccttgc ggacggtgcc gaacgccatg cgctcgaagt ggcgcaacgt gttcgtgcca
     1441 aggtgcagcc cgtacccggc gtccgcgaga ccgtcggcgg cgagctgcac gttcgagccc
     1501 cgtacggcca ggatgcggct catgcaccac gggcaggtgt ggacgttgtt gcagcggcac
     1561 gtgttgcccc acgtcgcctc gcccggcttc cacatcagct cggccgtccc ggcagtgagc
     1621 cgggtcccgc agcccttgaa cgcctcgttc agcgacaccg tctggtgccg gtcccgccgg
     1681 gcgaaccgct cgtcgcgcgg gtcctcccgc ccggctgtcg cggcaccctc gtttggggta
     1741 gaacccgttc cagttacagc gctctgacct gcagtggacg gagattttcc cttactacta
     1801 aagcccgcgt ccggattacc cgctgtagtc gtgcttgcta cgctgcgtga ctggtccgca
     1861 atgagacgct ttgcgcgctt tcggcaggcg tccgagcagt agattttggg gcgcttcccg
     1921 gggatgtgga cgatcggggt gccgcagtgg cacttcggtc cggcggggcg cggtggtgtc
     1981 gacgcgctgt tctctcgtac gctcgtcaca gagcaaacgt cctcactcgg catgctgcgc
     2041 cggttcgggg gcggcgagcc cgggaggcca atcccgggct cgtgccattt ctgggtcctg
     2101 ttgatcctgg cattggtgtg gccgttcatt gcccctgctc gctcctgacg cgccgataga
     2161 cgtccgatac gcccggtgct ggtgggattt gataggtcgg aagaagcccc gcccgggcct
     2221 gggcggggct tcctgtgcgt caggacctcc tcgtcgtgag cctcttcggc ctatggacgg
     2281 agtgacctcg tgatccgtta cagccgcgcg cgctcgcgta gagcggtctc atcagttcca
     2341 cgaacggtcc tcttcgcaga tcagggcgtt ggggcggagt ctcaccaagg actacgtctg
     2401 ctggcgattt ccgttacacc ccgggcggtg gccggcgcac acgcgcgccc gcgttgggca
     2461 gtgcagaaag tgcagaaacc taggcgctga tggtccaggt ccacggttcg tcgtcggcgg
     2521 cggcgcgggc ggcggcgtcg gccagggcgc gggcgagacc ggctacggcg ggcttgatgc
     2581 gccggttgcg ggcgaccttg agcagctagt atgcaggtcg acggatcttt tccgctgcat
     2641 aaccctgctt cggggtcatt atagcgattt tttcggtata tccatccttt ttcgcacgat
     2701 atacaggatt ttgccaaagg gttcgtgtag actttccttg gtgtatccaa cggcgtcagc
     2761 cgggcaggat aggtgaagta ggcccacccg cgagcgggtg ttccttcttc actgtccctt
     2821 attcgcacct ggcggtgctc aacgggaatc ctgctctgcg aggctggccg gctaccgccg
     2881 gcgtaacaga tgagggcaag cggatggctg atgaaaccaa gccaaccagg aagggcagcc
     2941 cacctatcaa ggtgtactgc cttccagacg aacgaagagc gattgaggaa aaggcggcgg
     3001 cggccggcat gagcctgtcg gcctacctgc tggccgtcgg ccagggctac aaaatcacgg
     3061 gcgtcgtgga ctatgagcac gtccgcgagc tggccctgca ggcggaagtc aggtagacac
     3121 gacttccgct agtccttgca aggtctgctg acgtgaggcg gggcggtcgt ttttgaccgc
     3181 cctgccttcg tcatgtaggc tcgctcgctg tgcctggcgt gtcatcagac gcccaggtcc
     3241 cggtgccgtg aggcccgggc catcgagccg gtggtacgtg gctgcggtcc ccttgtgagg
     3301 gctgcgcgcc gtgtgctgtc cggcgcgcac agccttgaat ccacccgcgg gggccggccg
     3361 gtctccgtga gctcgagtag acgacggaga cgtaatggac aagaagtaca gcatcggcct
     3421 ggacatcggc accaacagcg tgggctgggc ggtcatcacc gacgagtaca aggtcccctc
     3481 caagaagttc aaggtcctgg gcaacaccga ccggcactcg atcaagaaga acctgatcgg
     3541 cgccctgctc ttcgacagcg gcgaaaccgc cgaggcgacc cgcctgaagc ggaccgcccg
     3601 tcgccgctac acccggcgca agaaccgcat ctgctacctg caggagatct tctccaacga
     3661 gatggccaag gtcgacgact cgttcttcca ccggctcgag gagagcttcc tggtggagga
     3721 ggacaagaag cacgagcgcc acccgatctt cggcaacatc gtcgacgagg tggcctacca
     3781 cgagaagtac cccaccatct accacctccg caagaagctg gtcgactcga ccgacaaggc
     3841 ggacctgcgg ctcatctacc tggccctcgc gcacatgatc aagttccgcg gccacttcct
     3901 catcgagggc gacctgaacc cggacaactc cgacgtcgac aagctcttca tccagctggt
     3961 gcagacctac aaccagctgt tcgaggagaa ccccatcaac gccagcggcg tcgacgccaa
     4021 ggcgatcctc tccgcgcgcc tgagcaagtc ccggcgcctg gagaacctca tcgcccagct
     4081 gccgggcgag aagaagaacg gcctcttcgg caacctgatc gcgctgtcgc tcggcctgac
     4141 ccccaacttc aagagcaact tcgacctggc cgaggacgcg aagctccagc tgtccaagga
     4201 cacctacgac gacgacctgg acaacctgct cgcccagatc ggcgaccagt acgcggacct
     4261 cttcctggcc gcgaagaacc tctcggacgc catcctgctc agcgacatcc tgcgggtcaa
     4321 caccgagatc accaaggccc cgctgtcggc gagcatgatc aagcggtacg acgagcacca
     4381 ccaggacctg accctgctca aggccctcgt gcgccagcag ctgcccgaga agtacaagga
     4441 gatcttcttc gaccagtcca agaacggcta cgccggctac atcgacggcg gcgcgtcgca
     4501 ggaggagttc tacaagttca tcaagccgat cctggagaag atggacggca ccgaggagct
     4561 gctcgtcaag ctgaaccgcg aggacctgct ccgcaagcag cggaccttcg acaacggctc
     4621 catcccgcac cagatccacc tgggcgagct ccacgccatc ctccggcgcc aggaggactt
     4681 ctaccccttc ctgaaggaca accgcgagaa gatcgagaag atcctgacct tccggatccc
     4741 gtactacgtc ggccccctgg cccgcggcaa ctcccggttc gcgtggatga cccggaagtc
     4801 ggaggaaacc atcaccccgt ggaacttcga ggaggtcgtg gacaagggcg cctccgcgca
     4861 gtcgttcatc gagcgcatga ccaacttcga caagaacctc ccgaacgaga aggtcctgcc
     4921 caagcacagc ctgctctacg agtacttcac cgtgtacaac gagctgacca aggtcaagta
     4981 cgtgaccgag ggcatgcgga agccggcctt cctgtccggc gagcagaaga aggcgatcgt
     5041 cgacctgctc ttcaagacca accgcaaggt caccgtgaag cagctgaagg aggactactt
     5101 caagaagatc gagtgcttcg actccgtcga gatctcgggc gtggaggacc gcttcaacgc
     5161 ctccctgggc acctaccacg acctgctcaa gatcatcaag gacaaggact tcctcgacaa
     5221 cgaggagaac gaggacatcc tggaggacat cgtcctcacc ctgaccctct tcgaggaccg
     5281 cgagatgatc gaggagcggc tcaagaccta cgcccacctg ttcgacgaca aggtgatgaa
     5341 gcagctgaag cggcgccggt acaccggctg gggccgcctc tcccggaagc tgatcaacgg
     5401 catccgggac aagcagagcg gcaagaccat cctggacttc ctcaagtccg acggcttcgc
     5461 caaccgcaac ttcatgcagc tcatccacga cgactcgctg accttcaagg aggacatcca
     5521 gaaggcccag gtgtccggcc agggcgacag cctccacgag cacatcgcca acctggcggg
     5581 ctccccggcg atcaagaagg gcatcctcca gaccgtcaag gtcgtggacg agctggtcaa
     5641 ggtgatgggc cgccacaagc ccgagaacat cgtgatcgag atggcccggg agaaccagac
     5701 cacccagaag ggccagaaga actcccgcga gcggatgaag cgcatcgagg agggcatcaa
     5761 ggagctcggc tcgcagatcc tgaaggagca cccggtcgag aacacccagc tccagaacga
     5821 gaagctgtac ctctactacc tgcagaacgg ccgcgacatg tacgtggacc aggagctcga
     5881 catcaaccgg ctgagcgact acgacgtcga ccacatcgtg ccgcagtcct tcctgaagga
     5941 cgactcgatc gacaacaagg tcctgacccg ctccgacaag aaccggggca agtccgacaa
     6001 cgtgccctcg gaggaggtcg tgaagaagat gaagaactac tggcgccagc tgctcaacgc
     6061 caagctcatc acccagcgca agttcgacaa cctgaccaag gccgagcggg gcggcctgtc
     6121 ggagctcgac aaggcgggct tcatcaagcg ccagctcgtc gaaacccggc agatcaccaa
     6181 gcacgtggcc cagatcctgg acagccggat gaacaccaag tacgacgaga acgacaagct
     6241 gatccgcgag gtcaaggtga tcaccctcaa gagcaagctg gtgtccgact tccgcaagga
     6301 cttccagttc tacaaggtcc gggagatcaa caactaccac cacgcccacg acgcgtacct
     6361 gaacgccgtc gtgggcaccg cgctgatcaa gaagtacccg aagctggagt ccgagttcgt
     6421 ctacggcgac tacaaggtct acgacgtgcg caagatgatc gccaagtcgg agcaggagat
     6481 cggcaaggcc accgcgaagt acttcttcta cagcaacatc atgaacttct tcaagaccga
     6541 gatcaccctg gccaacggcg agatccgcaa gcggcccctg atcgaaacca acggcgaaac
     6601 cggcgagatc gtctgggaca agggccgcga cttcgccacc gtccggaagg tgctgtccat
     6661 gccgcaggtc aacatcgtca agaaaaccga ggtgcagacc ggcggcttca gcaaggagtc
     6721 catcctcccc aagcgcaact cggacaagct gatcgcccgg aagaaggact gggacccgaa
     6781 gaagtacggc ggcttcgaca gccccaccgt cgcctactcc gtgctggtcg tggcgaaggt
     6841 cgagaagggc aagagcaaga agctgaagtc cgtgaaggag ctgctcggca tcaccatcat
     6901 ggagcgctcc tcgttcgaga agaacccgat cgacttcctg gaggccaagg gctacaagga
     6961 ggtcaagaag gacctcatca tcaagctgcc caagtactcg ctgttcgagc tcgagaacgg
     7021 ccgcaagcgg atgctcgcca gcgcgggcga gctgcagaag ggcaacgagc tggccctccc
     7081 gtccaagtac gtcaacttcc tgtacctcgc gtcccactac gagaagctga agggctcgcc
     7141 cgaggacaac gagcagaagc agctcttcgt ggagcagcac aagcactacc tggacgagat
     7201 catcgagcag atctcggagt tcagcaagcg ggtcatcctg gccgacgcga acctcgacaa
     7261 ggtgctgtcc gcctacaaca agcaccgcga caagccgatc cgggagcagg cggagaacat
     7321 catccacctg ttcaccctca ccaacctggg cgcccccgcc gcgttcaagt acttcgacac
     7381 caccatcgac cgcaagcggt acaccagcac caaggaggtc ctcgacgcga ccctgatcca
     7441 ccagtccatc accggcctgt acgaaacccg catcgacctc tcccagctcg gcggcgactg
     7501 agaattcaga tctacgcgtt ccccgcaaaa gcggcctttg actccctgca agcctcagcg
     7561 accgaatata tcggttatgc gtgggcgatg gttgttgtca ttgtcggcgc aactatcggt
     7621 atcaagctgt ttaagaaatt cacctcgaaa gcaagctgat aaaccgatac aattaaaggc
     7681 tccttttgga gccttttttt gctgctcctt cggtcggacg tgcgtctacg ggcaccttac
     7741 cgcagccgtc ggctgtgcga cacggacgga tcgggcgaac tggccgatgc tgggagaagc
     7801 gcgctgctgt acggcgcgca ccgggtgcgg agcccctcgg cgagcggtgt gaaacttctg
     7861 tgaatggcct gttcggttgc tttttttata cggctgccag ataaggcttg cagcatctgg
     7921 gcggctaccg ctatgatcgg ggcgttcctg caattcttag tgcgagtatc tgaaagggga
     7981 tacgcTCATC GTTCTCAATA CACCGgtttt agagctagaa atagcaagtt aaaataaggc
     8041 tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg ctttttactc catctggatt
     8101 tgttcagaac gctcggttgc cgccgggcgt tttttatGAC GGGCTGGAGA TCCTGCTGGA
     8161 GGCCGGCGTC CGCGCCGTCG TCCAGCCCGG CGGCTCGGTC CGCGACGAAC TGGTCGTCGA
     8221 GGCCGCCAAG AAGGCCGGCG TCACCATGTA CTTCACGGGG ACCCGCCACT TCTTCCACTG
     8281 ACCGCGACGC GAGACGGCCG AGGGCCGCGT CCCCGAGCGG GGGCGCGGCC CTCGGCCGTC
     8341 TCAGCTGCTC GCGCGGGTCA GTAACGCGGG CGGTTGAACC AGACGGAAGC CTGGCTGTTG
     8401 ACCATCGCGG CGAGGATGAT GCCGTAGATC GCCAGCGCGA CGATGCCGCC GAAGGTGGCC
     8461 GAGCCGCCCT CGCCCGCGAA GAGGTTGCTC AGCGAACCGA GCAGCATCAG GGAGGCGTAG
     8521 ACGATCGTCG TGATGCGTAT GCCGGAGCCG CCGGTGGAGA ACTTCACGCC GAGCACGATG
     8581 GCGAGGACGG CCAGCGCCAG CAGGAGGAAG ACCAGCACGT AGCCGAGGCC GACGAGGCCG
     8641 TTGGCGGCGT CCGTGTCCAT GCCGTTGGCG ACGTCCTTGG CCGCGCCGAT GGCGACACCG
     8701 AAAAGGAGGC CGACCAGGAT CTGGAGGCCG GCCACGATGA AGAGCAGCAC GCGGGCCGTC
     8761 TTCATCAGGC CCGGCATCTC GGGCTTCATC GGGTTCATGC CCGGGCCACC CGGGTACCCC
     8821 GGCTGACCGC CCTGCTGCGG GTAGCCGTAA CCGGGCTGGC CCGGCTGGCC GCCCTGCTGC
     8881 GGGTAGCCGT AGGCCGGCTG GCCCTGGGGG GCGCCGTAGC CGCCGGGCTG GCCCTGGGGC
     8941 GAGCCGTAGC CGCCGGGCTG GCCCTGCGGG GGACCGTAGG GGTTGTTGGG GTCGCCGAAA
     9001 CTCATGGCGG GATTCCTCCG TTGAAGCGCG GGGACGCGCG GCCCGCGCAG GAGGTTGCGA
     9061 TGAAAGACGT AAGCGGCGTG GACATACATA GTATACTCTG GTGGTTCGGT CTGTCTTACT
     9121 GCTCCCATTT TCTCGTCAAT AATGTCCATA CCAACGAGTA AAGGGTATAT GAAAGGAGTG
     9181 GATTTCACAT CGTATCTCCC CATAGACTTT CACATTTGTT GTAGTGGCAG AGTGGACCGG
     9241 ATGGCCGAAA ACGGCGGCCC GGGAAAGGCG TCGTCCCGGC CCCCGCGTCC GTACTCCGGC
     9301 CCGGATTGGC GGCGGAGGGC GCGCATCCGC GAGGATGGGG GTATGAGCGC CCAGATTCTC
     9361 GATGGCAAGG CCACCGCGGC CGCGATCAAG TCCGAGCTGG CCCAGCGTGT GGCCGCTCTC
     9421 CGAGCCCAGG GCGTGCTGCC CGGCCTCGGT ACCGTCCTGG TCGGCGAGGA CCCGGGCAGC
     9481 CAGAAGTACG TCGCGGGCAA GCACCGCGAC TGCGCCCAGG TCGGTATCGC CTCCATCCAG
     9541 CGCGAACTGC CCGCCACCGC CACCCAGGAG GAGATCGAGG CGGTCGTCCG GGAGCTCAAC
     9601 GAGGACCCGG CCTGCACCGG TTACATCGTG CAGCTGCCCC TGCCCAAGGG CATCGACGAG
     9661 AACCGCATCC TCGAACTCAT GGACCCGGCC AAGGACGCCG ACGGCCTCCA CCCGACCAAC
     9721 CTCGGCCGCC TCGTACTCAA CGAGCCCGCG CCGCTGCCCT GCACGCCGTT CGGCATCGTC
     9781 ACACTGCTGC GCCACTTCGG CGTCGAGATC AACGGCGCCG AGGTCGTCGT CGTCGGCCGC
     9841 GGCGTCACCA TCGGCCGCCC GATGCCGCTG CTGCTCACCC GCCGCTCCGA GAACGCCACG
     9901 GTCACCCAGT GCCACACCGG CACCCGCGAC CTCGCCGCCC ACCTGCGCCG CGCCGACATC
     9961 ATCGTGGCCG CCGCCGGGGT GCCGCACCTG GTCAAGCCCG AGGACGTGAA GCCGGGCGCG
    10021 GCCGTCCTCG ACGTCGGCGT CAGCCGCGAC GAGAACGGCA AGATCGTCGG CGACGTCCAC
    10081 CCCGGCGTCG CCGAGGTGGC CGGCTGGATC TCCCCCAACC CGGGCGGAGT CGGCCCGATG
    10141 ACCCGCGCCC AGCTCCTCGT CAACGTCGTC aggccaggaa ccgtaaaaag gccgcgttgc
    10201 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
    10261 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
    10321 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
    10381 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
    10441 ttcgctccaa gctgggctgt gtgcacgacc cccccgttca gcccgaccgc tgcgccttat
    10501 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
    10561 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
    10621 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
    10681 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
    10741 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
    10801 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
    10861 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttggtt catgtgcagc
    10921 tccatcagca aaaggggatg ataagtttat caccaccgac tatttgcaac agtgccgttg
    10981 atcgtgctat gatcgactga tgtcatcagc ggtggagtgc aatgtcgtgc aatacgaatg
    11041 gcgaaaagcc gagctcatcg gtcagcttct caaccttggg gttacccccg gcggtgtgct
    11101 gctggtccac agctccttcc gtagcgtccg gcccctcgaa gatgggccac ttggactgat
    11161 cgaggccctg cgtgctgcgc tgggtccggg agggacgctc gtcatgccct cgtggtcagg
    11221 tctggacgac gagccgttcg atcctgccac gtcgcccgtt acaccggacc ttggagttgt
    11281 ctctgacaca ttctggcgcc tgccaaatgt aaagcgcagc gcccatccat ttgcctttgc
    11341 ggcagcgggg ccacaggcag agcagatcat ctctgatcca ttgcccctgc cacctcactc
    11401 gcctgcaagc ccggtcgccc gtgtccatga actcgatggg caggtacttc tcctcggcgt
    11461 gggacacgat gccaacacga cgctgcatct tgccgagttg atggcaaagg ttccctatgg
    11521 ggtgccgaga cactgcacca ttcttcagga tggcaagttg gtacgcgtcg attatctcga
    11581 gaatgaccac tgctgtgagc gctttgcctt ggcggacagg tggctcaagg agaagagcct
    11641 tcagaaggaa ggtccagtcg gtcatgcctt tgctcggttg atccgctccc gcgacattgt
    11701 ggcgacagcc ctgggtcaac tgggccgaga tccgttgatc ttcctgcatc cgccagaggc
    11761 gggatgcgaa gaatgcgatg ccgctcgcca gtcgattggc tgagctcatg agcggagaac
    11821 gagatgacgt tggaggggca aggtcgcgct gattgctggg gcaacacgtg gagcggatcg
    11881 gggattgtct ttcttcagct cgctgatgat atgctgacgc tcaatgccgt ttggcctccg
    11941 actaacgaaa atcccgcatt tggacggctg atccgattgg cacggcggac ggcgaatggc
    12001 ggagcagacg ctcgtccggg ggcaatgaga tatgaaaaag cctgaactca ccgcgacgta
    12061 tcgatgtcga ggttcctcag gggagccacc ccagagaagc cctcggagct gagcggagct
    12121 atttccaaag ccatgccagc tagagacagt gcacaccgcc aagcatctgc aaaaccctcg
    12181 cctggaggga aagtgcaatg tacgtacgct ggtttccctc cagaaaggga ttctgcggct
    12241 tctcaacttt ggaagaagag gcggaacgaa ctgctgtttc agcctactca tgtgaggagg
    12301 ctggtccttt accctgatgc ccgagggcat cagggtaaag gaccagcctc gccaactagg
    12361 agcgctacca aggcgaacac aacccaggac ttggtagaac ccttcgccgg agaagttcag
    12421 actcatcggc atgagcaccc ctcgccgcgc actcggcacc ggtccgtcga ccaccaccag
    12481 gacgtcgttg tcgacgtcgg ccccgcggct cctgcccgcc gaacgcgtcg tcgtcgacgg
    12541 cctggtgctc atcgacgagc acccggagcc aggtgaaaag cgccgg
//