LOCUS       pPP_-_vector_barcoded   3302 bp ds-DNA     circular     17-SEP-2019
DEFINITION  .
SOURCE      synthetic DNA construct                                            
  ORGANISM  synthetic DNA construct                                            
FEATURES             Location/Qualifiers
     promoter        2..940
                     /label="AOX1 promoter"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    1264..1386
                     /label="BC-10"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    1239..1263
                     /label="Universal primer"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer_bind     complement(2714..2733)
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     promoter        1806..1853
                     /label="EM7 promoter"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     terminator      2312..2559
                     /label="CYC1 terminator"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     terminator      978..1224
                     /label="AOX1 terminator"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     CDS             1872..2246
                     /label="BleoR"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     rep_origin      complement(2634..3222)
                     /label="ori"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     promoter        1387..1798
                     /label="TEF1 promoter"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer_bind     complement(2328..2346)
                     /label="CYC1"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer          953..974
                     /label="Pp1"
                     /note="sequence: gaagaccggtcttgctagattc"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer          complement(934..952)
                     /label="Pp2"
                     /note="sequence: acgtgaattcctcgtttcg"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
ORIGIN
        1 agatctaaca tccaaagacg aaaggttgaa tgaaaccttt ttgccatccg acatccacag
       61 gtccattctc acacataagt gccaaacgca acaggagggg atacactagc agcagaccgt
      121 tgcaaacgca ggacctccac tcctcttctc ctcaacaccc acttttgcca tcgaaaaacc
      181 agcccagtta ttgggcttga ttggagctcg ctcattccaa ttccttctat taggctacta
      241 acaccatgac tttattagcc tgtctatcct ggcccccctg gcgaggttca tgtttgttta
      301 tttccgaatg caacaagctc cgcattacac ccgaacatca ctccagatga gggctttctg
      361 agtgtggggt caaatagttt catgttcccc aaatggccca aaactgacag tttaaacgct
      421 gtcttggaac ctaatatgac aaaagcgtga tctcatccaa gatgaactaa gtttggttcg
      481 ttgaaatgct aacggccagt tggtcaaaaa gaaacttcca aaagtcggca taccgtttgt
      541 cttgtttggt attgattgac gaatgctcaa aaataatctc attaatgctt agcgcagtct
      601 ctctatcgct tctgaacccc ggtgcacctg tgccgaaacg caaatgggga aacacccgct
      661 ttttggatga ttatgcattg tctccacatt gtatgcttcc aagattctgg tgggaatact
      721 gctgatagcc taacgttcat gatcaaaatt taactgttct aacccctact tgacagcaat
      781 atataaacag aaggaagctg ccctgtctta aacctttttt tttatcatca ttattagctt
      841 actttcataa ttgcgactgg ttccaattga caagcttttg attttaacga cttttaacga
      901 caacttgaga agatcaaaaa acaactaatt attcgaaacg aggaattcac gtgaagaccg
      961 gtcttgctag attctaatca agaggatgtc agaatgccat ttgcctgaga gatgcaggct
     1021 tcatttttga tactttttta tttgtaacct atatagtata ggattttttt tgtcattttg
     1081 tttcttctcg tacgagcttg ctcctgatca gcctatctcg cagctgatga atatcttgtg
     1141 gtaggggttt gggaaaatca ttcgagtttg atgtttttct tggtatttcc cactcctctt
     1201 cagagtacag aagattaagt gagaccttcg tttgtgcgTG GACATACATA GTATACTCTG
     1261 GTGTAGAGCA AGTAATATGT AGCGATAGCG TGGGGGGAAT AAAGTTAGTC GCCACTTTGT
     1321 CGGGTAGCAG ATAGTGTAAT CTATTCAAAC TCATAAAACT CGTCTTTATG CCCTTTGGTA
     1381 TCAATCgatc ccccacacac catagcttca aaatgtttct actccttttt tactcttcca
     1441 gattttctcg gactccgcgc atcgccgtac cacttcaaaa cacccaagca cagcatacta
     1501 aattttccct ctttcttcct ctagggtgtc gttaattacc cgtactaaag gtttggaaaa
     1561 gaaaaaagag accgcctcgt ttctttttct tcgtcgaaaa aggcaataaa aatttttatc
     1621 acgtttcttt ttcttgaaat tttttttttt agtttttttc tctttcagtg acctccattg
     1681 atatttaagt taataaacgg tcttcaattt ctcaagtttc agtttcattt ttcttgttct
     1741 attacaactt tttttacttc ttgttcatta gaaagaaagc atagcaatct aatctaaggg
     1801 gcggtgttga caattaatca tcggcatagt atatcggcat agtataatac gacaaggtga
     1861 ggaactaaac catggccaag ttgaccagtg ccgttccggt gctcaccgcg cgcgacgtcg
     1921 ccggagcggt cgagttctgg accgaccggc tcgggttctc ccgggacttc gtggaggacg
     1981 acttcgccgg tgtggtccgg gacgacgtga ccctgttcat cagcgcggtc caggaccagg
     2041 tggtgccgga caacaccctg gcctgggtgt gggtgcgcgg cctggacgag ctgtacgccg
     2101 agtggtcgga ggtcgtgtcc acgaacttcc gggacgcctc cgggccggcc atgaccgaga
     2161 tcggcgagca gccgtggggg cgggagttcg ccctgcgcga cccggccggc aactgcgtgc
     2221 acttcgtggc cgaggagcag gactgacacg tccgacggcg gcccacgggt cccaggcctc
     2281 ggagatccgt cccccttttc ctttgtcgat atcatgtaat tagttatgtc acgcttacat
     2341 tcacgccctc cccccacatc cgctctaacc gaaaaggaag gagttagaca acctgaagtc
     2401 taggtcccta tttatttttt tatagttatg ttagtattaa gaacgttatt tatatttcaa
     2461 atttttcttt tttttctgta cagacgcgtg tacgcatgta acattatact gaaaaccttg
     2521 cttgagaagg ttttgggacg ctcgaaggct ttaatttgca agctggagac caacatgtga
     2581 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     2641 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     2701 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     2761 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     2821 ctttctcaat gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     2881 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     2941 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     3001 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     3061 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     3121 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     3181 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     3241 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     3301 tc
//