LOCUS       pSC-X2-CreLox-kanMX_bar 5527 bp ds-DNA     circular     12-JUL-2020
DEFINITION  .
SOURCE      synthetic DNA construct                                            
  ORGANISM  synthetic DNA construct                                            
FEATURES             Location/Qualifiers
     CDS             complement(join(5526..5527,1..859))
                     /label="AmpR"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    1318..1848
                     /label="HR1"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer_bind     993..1012
                     /label="Kan-R"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     protein_bind    1997..2030
                     /label="loxP"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     terminator      2060..2257
                     /label="TEF terminator"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    1874..1996
                     /label="BC2"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer_bind     2986..3005
                     /label="Kan-R"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    complement(3493..3983)
                     /label="HR2"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     protein_bind    complement(4025..4058)
                     /label="FRT (minimal)"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     protein_bind    1244..1277
                     /label="FRT (minimal)"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     promoter        complement(3073..3416)
                     /label="TEF promoter"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer_bind     complement(4528..4544)
                     /label="M13 fwd"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     CDS             complement(2263..3072)
                     /label="KanR"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     promoter        complement(860..951)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    1849..1873
                     /label="Universal Primer"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer_bind     622..641
                     /label="Amp-R"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer_bind     complement(4643..4660)
                     /label="L4440"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     gene            complement(2060..3416)
                     /label="kanMX"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer_bind     1018..1037
                     /label="pENTR-R"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     protein_bind    3459..3492
                     /label="loxP"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer_bind     complement(4894..4913)
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     rep_origin      complement(4814..5402)
                     /label="ori"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer          complement(3966..3983)
                     /label="BC_180"
                     /note="sequence: tctcgccaaggcattacc"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          1318..1338
                     /label="BC_179"
                     /note="sequence: cgtctatgaggagactgttag"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
ORIGIN
        1 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
       61 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
      121 gcgctgcgat gataccgcga gaaccacgct caccggctcc ggatttatca gcaataaacc
      181 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
      241 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
      301 ttgttgccat cgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
      361 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
      421 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
      481 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
      541 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
      601 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
      661 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
      721 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
      781 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
      841 ggaaatgttg aatactcata ttcttccttt ttcaatatta ttgaagcatt tatcagggtt
      901 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggtca
      961 gtgttacaac caattaacca attctgaaca ttatcgcgag cccatttata cctgaatatg
     1021 gctcataaca ccccttgttt gcctggcggc agtagcgcgg tggtcccacc tgaccccatg
     1081 ccgaactcag aagtgaaacg ccgtagcgcc gatggtagtg tggggactcc ccatgcgaga
     1141 gtagggaact gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
     1201 cccgggctaa ttatggggtg tcgcccttat tcgactctat agtgaagttc ctattctcta
     1261 gaaagtatag gaacttctga agtggggatt taaatgcggc cgcgctgagg gtttaatcgt
     1321 ctatgaggag actgttagtt ggatatcagt aatgagacga aaaagctcga aatgaatgga
     1381 tatattcttt ttgctactgg caactgttga atatttaatg ttaaaacaaa ctaactgagg
     1441 tatattcgta tctgtatgta cacatatact atatacagga aaagataagc aagagagagg
     1501 atatcaacta cgagagcgat cgattatata tcaaaagctg tccgctttgc cacccataat
     1561 cggcgcttag tttcggagtt caatcataat tctaccacct tacactcaac ttactcttta
     1621 actcctatag tataatatcg ccactgaccc catattaaaa aatttttttg ctcgatcttc
     1681 tatcctcttt aggttaattg tcgctgttat tgtctagatt ttttctcgga gatggcgcat
     1741 ctatttgccg tcaaaagatc ctctcatacc atattaagta aattgcctcc atttcttttt
     1801 cctcgggcag agaaactcgc aggcaacttg ctctcgaagt ggtcacgtTG GACATACATA
     1861 GTATACTCTG GTGAATGCTT ATCGTGTAGT AGGGTAGTTT GGATAGCGAG TTGAACAAGG
     1921 GGTCGGTCAT ACAGACTTTG TGGGGAGTAA AGTCAGCGAA AGGGGTCTGT CGTCTTTAGA
     1981 AGCGAACCAT AGTGGCataa cttcgtatag catacattat acgaagttat ggatggcggc
     2041 gttagtatcg aatcgacagc agtatagcga ccagcattca catacgattg acgcatgata
     2101 ttactttctg cgcacttaac ttcgcatctg ggcagatgat gtcgaggcga aaaaaaatat
     2161 aaatcacgct aacatttgat taaaatagaa caactacaat ataaaaaaac tatacaaatg
     2221 acaagttctt gaaaacaaga atctttttat tgtcagtact gattagaaaa actcatcgag
     2281 catcaaatga aactgcaatt tattcatatc aggattatca ataccatatt tttgaaaaag
     2341 ccgtttctgt aatgaaggag aaaactcacc gaggcagttc cataggatgg caagatcctg
     2401 gtatcggtct gcgattccga ctcgtccaac atcaatacaa cctattaatt tcccctcgtc
     2461 aaaaataagg ttatcaagtg agaaatcacc atgagtgacg actgaatccg gtgagaatgg
     2521 caaaagctta tgcatttctt tccagacttg ttcaacaggc cagccattac gctcgtcatc
     2581 aaaatcactc gcatcaacca aaccgttatt cattcgtgat tgcgcctgag cgagacgaaa
     2641 tacgcgatcg ctgttaaaag gacaattaca aacaggaatc gaatgcaacc ggcgcaggaa
     2701 cactgccagc gcatcaacaa tattttcacc tgaatcagga tattcttcta atacctggaa
     2761 tgctgttttg ccggggatcg cagtggtgag taaccatgca tcatcaggag tacggataaa
     2821 atgcttgatg gtcggaagag gcataaattc cgtcagccag tttagtctga ccatctcatc
     2881 tgtaacatca ttggcaacgc tacctttgcc atgtttcaga aacaactctg gcgcatcggg
     2941 cttcccatac aatcgataga ttgtcgcacc tgattgcccg acattatcgc gagcccattt
     3001 atacccatat aaatcagcat ccatgttgga atttaatcgc ggcctcgaaa cgtgagtctt
     3061 ttccttaccc atggttgttt atgttcggat gtgatgtgag aactgtatcc tagcaagatt
     3121 ttaaaaggaa gtatatgaaa gaagaacctc agtggcaaat cctaaccttt tatatttctc
     3181 tacaggggcg cggcgtgggg acaattcaac gcgtctgtga ggggagcgtt tccctgctcg
     3241 caggtctgca gcgaggagcc gtaatttttg cttcgcgccg tgcggccatc aaaatgtatg
     3301 gatgcaaatg attatacatg gggatgtatg ggctaaatgt acgggcgaca gtcacatcat
     3361 gcccctgagc tgcgcacgtc aagactgtca aggagggtat tctgggcctc catgtcgctg
     3421 gccgggtgac ccggcgggga cgaggcaagc taaacagaat aacttcgtat agcatacatt
     3481 atacgaagtt atcctgcata atcggcctca cagagggatc ccgttaccca tctatgctga
     3541 agatttatca tactattcct ccgctcgttt cttttttcag tgaggtgtgt cgtgaaagaa
     3601 aacccacaat taaactttca acaaccgggc gactaggaag agagtagtgg gcgcggatga
     3661 cgaaggctaa ggtcacttct tcgtttcctt tattggggtt tccgtgtagc cttcccctga
     3721 atagtgtggg acgttttatg agaagccgta agaaataggc aaattgagtt atgacaagta
     3781 gacatgatgc cgcagccttg cctgacttta cgtctccttc atgaataagt ttttctatcg
     3841 agttcttttc cttttttcgc cttaattagc tcaattaagc ctgtcctcac tacttttctt
     3901 tttcttatcg gctttgtgcc acacctaacc ttcgaatgct gttttattcc gttcttacat
     3961 gggatggtaa tgccttggcg agattaagac ctcagcgcgg ccgcaaattt aaataaaatg
     4021 aagtgaagtt cctatacttt ctagagaata ggaacttcta tagtgagtcg aataagggcg
     4081 acacaaaatt tattctaaat gcataataaa tactgataac atcttatagt ttgtattata
     4141 ttttgtatta tcgttgacat gtataatttt gatatcaaaa actgattttc cctttattat
     4201 tttcgagatt tattttctta attctcttta acaaactaga aatattgtat atacaaaaaa
     4261 tcataaataa tagatgaata gtttaattat aggtgttcat caatcgaaaa agcaacgtat
     4321 cttatttaaa gtgcgttgct tttttctcat ttataaggtt aaataattct catatatcaa
     4381 gcaaagtgac aggcgccctt aaatattctg acaaatgctc tttccctaaa ctccccccat
     4441 aaaaaaaccc gccgaagcgg gtttttacgt tatttgcgga ttaacgatta ctcgttatca
     4501 gaaccgccca gggggcccga gcttaagact ggccgtcgtt ttacaacaca gaaagagttt
     4561 gtagaaacgc aaaaaggcca tccgtcaggg gccttctgct tagtttgatg cctggcagtt
     4621 ccctactctc gccttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     4681 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     4741 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     4801 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     4861 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     4921 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     4981 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     5041 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     5101 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     5161 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     5221 tgaagtggtg ggctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     5281 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     5341 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     5401 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgac gcgcgcgtaa
     5461 ctcacgttaa gggattttgg tcatgagctt gcgccgtccc gtcaagtcag cgtaatgctc
     5521 tgctttt
//