LOCUS       pBF3060_natMX           7326 bp ds-DNA     circular     23-JUN-2018
DEFINITION  .
SOURCE      synthetic DNA construct                                            
  ORGANISM  synthetic DNA construct                                            
FEATURES             Location/Qualifiers
     primer_bind     7203..7222
                     /label="F1ori-R"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer_bind     complement(1643..1661)
                     /label="CYC1"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     promoter        2..443
                     /label="GAL1 promoter"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     rep_origin      complement(2115..2703)
                     /label="ori"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     terminator      4752..4949
                     /label="TEF terminator"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     terminator      1627..1874
                     /label="CYC1 terminator"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     protein_bind    2..119
                     /label="UAS"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer_bind     414..437
                     /label="GAL1"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     rep_origin      5905..6785
                     /label="2u ori"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer_bind     complement(1944..1961)
                     /label="L4440"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     gene            3830..4949
                     /label="natMX6"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     CDS             complement(2874..3734)
                     /label="AmpR"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer_bind     complement(2195..2214)
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    484..1617
                     /label="creA"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     CDS             4174..4746
                     /label="NrsR"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     promoter        3830..4173
                     /label="TEF promoter"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     rep_origin      complement(6854..7309)
                     /label="f1 ori"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
ORIGIN
        1 acggattaga agccgccgag cgggtgacag ccctccgaag gaagactctc ctccgtgcgt
       61 cctcgtcttc accggtcgcg ttcctgaaac gcagatgtgc ctcgcgccgc actgctccga
      121 acaataaaga ttctacaata ctagctttta tggttatgaa gaggaaaaat tggcagtaac
      181 ctggccccac aaaccttcaa atgaacgaat caaattaaca accataggat gataatgcga
      241 ttagtttttt agccttattt ctggggtaat taatcagcga agcgatgatt tttgatctat
      301 taacagatat ataaatgcaa aaactgcata accactttaa ctaatacttt caacattttc
      361 ggtttgtatt acttcttatt caaatgtaat aaaagtatca acaaaaaatt gttaatatac
      421 ctctatactt taacgtcaag gagaaaaaac cccggatcgg actactagca gccatggaaa
      481 aaaatgtcta atttgttgac tgttcatcaa aatttgccag ctttgccagt tgatgcaacg
      541 tctgatgagg taagaaagaa tttgatggat atgtttagag atagacaagc attctctgaa
      601 catacttgga aaatgttgtt gtctgtttgt agatcttggg ctgcttggtg taaattaaat
      661 aatagaaaat ggtttccagc tgaaccagaa gatgttagag attatttgtt gtatttgcaa
      721 gctagaggtt tggctgttaa aactatacaa caacatttgg gtcaattaaa tatgttgcat
      781 aggagatctg gtttgcctag accatctgat tctaatgctg tatctttggt tatgagaagg
      841 attagaaaag aaaatgttga tgcaggtgaa agagctaaac aagcattggc gtttgaaaga
      901 actgattttg atcaagttag atctttgatg gaaaattctg atagatgtca agatataaga
      961 aacttggctt tcttgggaat tgcttataat acattgttga gaattgctga aattgctaga
     1021 attagagtta aagacatttc tagaactgat ggtggtagaa tgttgattca tattggtaga
     1081 actaaaacat tggtttcaac tgctggcgtt gagaaagcat tgtctttggg tgttactaaa
     1141 ttggttgaaa gatggatttc tgtatctggt gtggctgatg acccaaataa ttatttattt
     1201 tgtagagtta gaaagaatgg tgttgcggct ccatctgcta catctcagct ctctacccgt
     1261 gccttggaag gaatttttga agcaactcat agattgattt atggtgctaa agatgattct
     1321 ggtcaaagat atttagcatg gtctggtcat tctgctagag ttggtgccgc tagagatatg
     1381 gctagagctg gggtatctat tcctgaaatt atgcaagctg gtggttggac taatgttaac
     1441 atcgtcatga actatattag aaatttggat tctgaaactg gtgcaatggt tagactcttg
     1501 gaagatggtg atcccgggaa gcttggtacc gagctcggat ccactagtaa cggccgccag
     1561 tgtgctggaa ttctgcagat atccatcaca ctggcggccg ctcgagcatg catctagagg
     1621 gccgcatcat gtaattagtt atgtcacgct tacattcacg ccctcccccc acatccgctc
     1681 taaccgaaaa ggaaggagtt agacaacctg aagtctaggt ccctatttat ttttttatag
     1741 ttatgttagt attaagaacg ttatttatat ttcaaatttt tctttttttt ctgtacagac
     1801 gcgtgtacgc atgtaacatt atactgaaaa ccttgcttga gaaggttttg ggacgctcga
     1861 aggctttaat ttgcggccct gcattaatga atcggccaac gcgcggggag aggcggtttg
     1921 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     1981 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     2041 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagc ccaggaaccg taaaaaggcc
     2101 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     2161 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     2221 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     2281 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     2341 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     2401 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     2461 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     2521 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg
     2581 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     2641 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     2701 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     2761 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     2821 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     2881 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     2941 tgactccccg tcgtgtagat aactacgata cgggagcgct taccatctgg ccccagtgct
     3001 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     3061 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat tcagtctatt
     3121 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     3181 ggcattgcta caggcatcgt ggtgtcactc tcgtcgtttg gtatggcttc attcagctcc
     3241 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     3301 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     3361 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     3421 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     3481 ccggcgtcaa tacgggataa tagtgtatca catagcagaa ctttaaaagt gctcatcatt
     3541 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     3601 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     3661 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     3721 tgttgaatac tcatactctt cctttttcaa tgggtaataa ctgatataat taaattgaag
     3781 ctctaatttg tgagtttagt atacatgcat ttacttataa tacagttttg acatggaggc
     3841 ccagaatacc ctccttgaca gtcttgacgt gcgcagctca ggggcatgat gtgactgtcg
     3901 cccgtacatt tagcccatac atccccatgt ataatcattt gcatccatac attttgatgg
     3961 ccgcacggcg cgaagcaaaa attacggctc ctcgctgcag acctgcgagc agggaaacgc
     4021 tcccctcaca gacgcgttga attgtcccca cgccgcgccc ctgtagagaa atataaaagg
     4081 ttaggatttg ccactgaggt tcttctttca tatacttcct tttaaaatct tgctaggata
     4141 cagttctcac atcacatccg aacataaaca accatgggta ccactcttga cgacacggct
     4201 taccggtacc gcaccagtgt cccgggggac gccgaggcca tcgaggcact ggatgggtcc
     4261 ttcaccaccg acaccgtttt ccgcgtcacc accaccgggg acggcttcac cctgcgggag
     4321 gtgccggtgg acccgcccct gaccaaggtg ttccccgacg acgaatcgga cgacgaatcg
     4381 gacgacgggg aggacggcga cccggactcc cggacgttcg tcgcgtacgg ggacgacggc
     4441 gacctggcgg gcttcgtggt cgtctcgtac tccggctgga accgccggct gaccgtcgag
     4501 gacatcgagg tcgccccgga gcaccggggg cacggggtcg ggcgcgcgtt gatggggctc
     4561 gcgacggagt tcgcccgcga gcggggcgcc gggcacctct ggctggaggt caccaacgtc
     4621 aacgcaccgg cgatccacgc gtaccggcgg atggggttca ccctctgcgg cctggacacc
     4681 gccctgtacg acggcaccgc ctcggacggc gagcaggcgc tctacatgag catgccctgc
     4741 ccctaatcag tactgacaat aaaaagattc ttgttttcaa gaacttgtca tttgtatagt
     4801 ttttttatat tgtagttgtt ctattttaat caaatgttag cgtgatttat attttttttc
     4861 gcctcgacat catctgccca gatgcgaagt taagtgcgca gaaagtaata tcatgcgtca
     4921 atcgtatgtg aatgctggtc gctatactgt tgaaaagcta gcttatcgat gataagctgt
     4981 caaagatgag aattaattcc acggactata gactatacta gatactccgt ctactgtacg
     5041 atacacttcc gctcaggtcc ttgtccttta acgaggcctt accactcttt tgttactcta
     5101 ttgatccagc tcagcaaagg cagtgtgatc taagattcta tcttcgcgat gtagtaaaac
     5161 tagctagacc gagaaagaga ctagaaatgc aaaaggcact tctacaatgg ctgccatcat
     5221 tattatccga tgtgacgctg cagcttctca atgatattcg aatacgcttt gaggagatac
     5281 agcctaatat ccgacaaact gttttacaga tttacgatcg tagataagct gtcaaagatg
     5341 agaattaatt ccacggacta tagactatac tagatactcc gtctactgta cgatacactt
     5401 ccgctcaggt ccttgtcctt taacgaggcc ttaccactct tttgttactc tattgatcca
     5461 gctcagcaaa ggcagtgtga tctaagattc tatcttcgcg atgtagtaaa actagctaga
     5521 ccgagaaaga gactagaaat gcaaaaggca cttctacaat ggctgccatc attattatcc
     5581 gatgtgacgc tgcagcttct caatgatatt cgaatacgct ttgaggagat acagcctaat
     5641 atccgacaaa ctgttttaca gatttacgat cgtacttgtt acccatcatt gaattttgaa
     5701 catccgaacc tgggagtttt ccctgaaaca gatagtatat ttgaacctgt ataataatat
     5761 atagtctagc gctttacgga agacaatgta tgtatttcgg ttcctggaga aactattgca
     5821 tctattgcat aggtaatctt gcacgtcgca tccccggttc attttctgcg tttccatctt
     5881 gcacttcaat agcatatctt tgttaacgaa gcatctgtgc ttcattttgt agaacaaaaa
     5941 tgcaacgcga gagcgctaat ttttcaaaca aagaatctga gctgcatttt tacagaacag
     6001 aaatgcaacg cgaaagcgct attttaccaa cgaagaatct gtgcttcatt tttgtaaaac
     6061 aaaaatgcaa cgcgacgaga gcgctaattt ttcaaacaaa gaatctgagc tgcattttta
     6121 cagaacagaa atgcaacgcg agagcgctat tttaccaaca aagaatctat acttcttttt
     6181 tgttctacaa aaatgcatcc cgagagcgct atttttctaa caaagcatct tagattactt
     6241 tttttctcct ttgtgcgctc tataatgcag tctcttgata actttttgca ctgtaggtcc
     6301 gttaaggtta gaagaaggct actttggtgt ctattttctc ttccataaaa aaagcctgac
     6361 tccacttccc gcgtttactg attactagcg aagctgcggg tgcatttttt caagataaag
     6421 gcatccccga ttatattcta taccgatgtg gattgcgcat actttgtgaa cagaaagtga
     6481 tagcgttgat gattcttcat tggtcagaaa attatgaacg gtttcttcta ttttgtctct
     6541 atatactacg tataggaaat gtttacattt tcgtattgtt ttcgattcac tctatgaata
     6601 gttcttacta caattttttt gtctaaagag taatactaga gataaacata aaaaatgtag
     6661 aggtcgagtt tagatgcaag ttcaaggagc gaaaggtgga tgggtaggtt atatagggat
     6721 atagcacaga gatatatagc aaagagatac ttttgagcaa tgtttgtgga agcggtattc
     6781 gcaatgggaa gctccacccc ggttgataat cagaaaagcc ccaaaaacag gaagattgta
     6841 taagcaaata tttaaattgt aaacgttaat attttgttaa aattcgcgtt aaatttttgt
     6901 taaatcagct cattttttaa cgaatagccc gaaatcggca aaatccctta taaatcaaaa
     6961 gaatagaccg agatagggtt gagtgttgtt ccagtttcca acaagagtcc actattaaag
     7021 aacgtggact ccaacgtcaa agggcgaaaa agggtctatc agggcgatgg cccactacgt
     7081 gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcagt aaatcggaag
     7141 ggtaaacgga tgcccccatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag
     7201 gaagggaaga aagcgaaagg agcgggggct agggcggtgg gaagtgtagg ggtcacgctg
     7261 ggcgtaacca ccacacccgc cgcgcttaat ggggcgctac agggcgcgtg gggatgatcc
     7321 actagt
//