LOCUS       pCfB3020(gRNA_X-2)      5293 bp ds-DNA     circular     19-SEP-2018
DEFINITION  .
SOURCE      synthetic DNA construct                                            
  ORGANISM  synthetic DNA construct                                            
FEATURES             Location/Qualifiers
     primer_bind     4648..4667
                     /label="Amp-R"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     rep_origin      join(5017..5293,1..1066)
                     /label="2u ori"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     promoter        2606..2874
                     /label="SNR52 promoter"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer_bind     complement(3346..3365)
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_RNA        2895..2969
                     /label="gRNA scaffold"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             complement(4025..4885)
                     /label="AmpR"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     terminator      2231..2428
                     /label="TEF terminator"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     protein_bind    complement(687..734)
                     /label="FRT"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     rep_origin      complement(3266..3854)
                     /label="ori"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     promoter        complement(4886..4990)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer_bind     1107..1125
                     /label="pBRforEco"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer_bind     complement(1163..1185)
                     /label="pGEX 3'"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     gene            1309..2428
                     /label="natMX6"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     promoter        1309..1652
                     /label="TEF promoter"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer_bind     complement(3095..3112)
                     /label="L4440"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     CDS             1653..2225
                     /label="NrsR"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     terminator      2974..2993
                     /label="SUP4 terminator"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
ORIGIN
        1 ctacaaaaat gcatcccgag agcgctattt ttctaacaaa gcatcttaga ttactttttt
       61 tctcctttgt gcgctctata atgcagtctc ttgataactt tttgcactgt aggtccgtta
      121 aggttagaag aaggctactt tggtgtctat tttctcttcc ataaaaaaag cctgactcca
      181 cttcccgcgt ttactgatta ctagcgaagc tgcgggtgca ttttttcaag ataaaggcat
      241 ccccgattat attctatacc gatgtggatt gcgcatactt tgtgaacaga aagtgatagc
      301 gttgatgatt cttcattggt cagaaaatta tgaacggttt cttctatttt gtctctatat
      361 actacgtata ggaaatgttt acattttcgt attgttttcg attcactcta tgaatagttc
      421 ttactacaat ttttttgtct aaagagtaat actagagata aacataaaaa atgtagaggt
      481 cgagtttaga tgcaagttca aggagcgaaa ggtggatggg taggttatat agggatatag
      541 cacagagata tatagcaaag agatactttt gagcaatgtt tgtggaagcg gtattcgcaa
      601 tattttagta gctcgttaca gtccggtgcg tttttggttt tttgaaagtg cgtcttcaga
      661 gcgcttttgg ttttcaaaag cgctctgaag ttcctatact ttctagagaa taggaacttc
      721 ggaataggaa cttcaaagcg tttccgaaaa cgagcgcttc cgaaaatgca acgcgagctg
      781 cgcacataca gctcactgtt cacgtcgcac ctatatctgc gtgttgcctg tatatatata
      841 tacatgagaa gaacggcata gtgcgtgttt atgcttaaat gcgtacttat atgcgtctat
      901 ttatgtagga tgaaaggtag tctagtacct cctgtgatat tatcccattc catgcggggt
      961 atcgtatgct tccttcagca ctacccttta gctgttctat atgctgccac tcctcaattg
     1021 gattagtctc atccttcaat gctatcattt cctttgatat tggatcatac taagaaacca
     1081 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc
     1141 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
     1201 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggctgcattt
     1261 aaatgatctg tttagcttgc ctcgtccccg ccgggtcacc cggccagcga catggaggcc
     1321 cagaataccc tccttgacag tcttgacgtg cgcagctcag gggcatgatg tgactgtcgc
     1381 ccgtacattt agcccataca tccccatgta taatcatttg catccataca ttttgatggc
     1441 cgcacggcgc gaagcaaaaa ttacggctcc tcgctgcaga cctgcgagca gggaaacgct
     1501 cccctcacag acgcgttgaa ttgtccccac gccgcgcccc tgtagagaaa tataaaaggt
     1561 taggatttgc cactgaggtt cttctttcat atacttcctt ttaaaatctt gctaggatac
     1621 agttctcaca tcacatccga acataaacaa ccatgggtac cactcttgac gacacggctt
     1681 accggtaccg caccagtgtc ccgggggacg ccgaggccat cgaggcactg gatgggtcct
     1741 tcaccaccga caccgttttc cgcgtcacca ccaccgggga cggcttcacc ctgcgggagg
     1801 tgccggtgga cccgcccctg accaaggtgt tccccgacga cgaatcggac gacgaatcgg
     1861 acgacgggga ggacggcgac ccggactccc ggacgttcgt cgcgtacggg gacgacggcg
     1921 acctggcggg cttcgtggtc gtctcgtact ccggctggaa ccgccggctg accgtcgagg
     1981 acatcgaggt cgccccggag caccgggggc acggggtcgg gcgcgcgttg atggggctcg
     2041 cgacggagtt cgcccgcgag cggggcgccg ggcacctctg gctggaggtc accaacgtca
     2101 acgcaccggc gatccacgcg taccggcgga tggggttcac cctctgcggc ctggacaccg
     2161 ccctgtacga cggcaccgcc tcggacggcg agcaggcgct ctacatgagc atgccctgcc
     2221 cctaatcagt actgacaata aaaagattct tgttttcaag aacttgtcat ttgtatagtt
     2281 tttttatatt gtagttgttc tattttaatc aaatgttagc gtgatttata ttttttttcg
     2341 cctcgacatc atctgcccag atgcgaagtt aagtgcgcag aaagtaatat catgcgtcaa
     2401 tcgtatgtga atgctggtcg ctatactgct gtcgattcga tactaacgcc gccatccagt
     2461 gtcgaaaacg agctgcggcc gcctacaggg cgcgtcgcgc cattcgccat tcaggctgcg
     2521 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggaatgcgt
     2581 gcgataggga acaaaagctg gagcttcttt gaaaagataa tgtatgatta tgctttcact
     2641 catatttata cagaaacttg atgttttctt tcgagtatat acaaggtgat tacatgtacg
     2701 tttgaagtac aactctagat tttgtagtgc cctcttgggc tagcggtaaa ggtgcgcatt
     2761 ttttcacacc ctacaatgtt ctgttcaaaa gattttggtc aaacgctgta gaagtgaaag
     2821 ttggtgcgca tgtttcggcg ttcgaaactt ctccgcagtg aaagataaat gatcctctcg
     2881 aagtggtcac gtgcgtttta gagctagaaa tagcaagtta aaataaggct agtccgttat
     2941 caacttgaaa aagtggcacc gagtcggtgg tgcttttttt gttttttatg tcttcgagtc
     3001 atgtaattag ttaatcgcgt gcattccagc tgcattaatg aatcggccaa cgcgcgggga
     3061 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     3121 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     3181 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     3241 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     3301 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     3361 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     3421 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     3481 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     3541 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     3601 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     3661 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
     3721 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     3781 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     3841 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     3901 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     3961 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     4021 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     4081 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     4141 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     4201 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     4261 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     4321 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     4381 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     4441 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     4501 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     4561 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     4621 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     4681 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     4741 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     4801 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     4861 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     4921 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     4981 gggttccgcg cacatttccc cgaaaagtgc cacctgaacg aagcatctgt gcttcatttt
     5041 gtagaacaaa aatgcaacgc gagagcgcta atttttcaaa caaagaatct gagctgcatt
     5101 tttacagaac agaaatgcaa cgcgaaagcg ctattttacc aacgaagaat ctgtgcttca
     5161 tttttgtaaa acaaaaatgc aacgcgagag cgctaatttt tcaaacaaag aatctgagct
     5221 gcatttttac agaacagaaa tgcaacgcga gagcgctatt ttaccaacaa agaatctata
     5281 cttctttttt gtt
//