LOCUS       pSC-X2-BC               4031 bp ds-DNA     circular     13-JUL-2020
DEFINITION  .
SOURCE      synthetic DNA construct                                            
  ORGANISM  synthetic DNA construct                                            
FEATURES             Location/Qualifiers
     misc_feature    1318..1848
                     /label="HR1"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    complement(1997..2487)
                     /label="HR2"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer_bind     993..1012
                     /label="Kan-R"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer_bind     1018..1037
                     /label="pENTR-R"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     rep_origin      complement(3318..3906)
                     /label="ori"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    1849..1873
                     /label="Universal Primer"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     protein_bind    1244..1277
                     /label="FRT (minimal)"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer_bind     622..641
                     /label="Amp-R"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer_bind     complement(3147..3164)
                     /label="L4440"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     promoter        complement(860..951)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer_bind     complement(3032..3048)
                     /label="M13 fwd"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    1874..1996
                     /label="BC14"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     CDS             complement(join(4030..4031,1..859))
                     /label="AmpR"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     protein_bind    complement(2529..2562)
                     /label="FRT (minimal)"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer_bind     complement(3398..3417)
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer          complement(2470..2487)
                     /label="BC_180"
                     /note="sequence: tctcgccaaggcattacc"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          1318..1338
                     /label="BC_179"
                     /note="sequence: cgtctatgaggagactgttag"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
ORIGIN
        1 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
       61 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
      121 gcgctgcgat gataccgcga gaaccacgct caccggctcc ggatttatca gcaataaacc
      181 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
      241 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
      301 ttgttgccat cgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
      361 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
      421 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
      481 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
      541 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
      601 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
      661 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
      721 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
      781 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
      841 ggaaatgttg aatactcata ttcttccttt ttcaatatta ttgaagcatt tatcagggtt
      901 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggtca
      961 gtgttacaac caattaacca attctgaaca ttatcgcgag cccatttata cctgaatatg
     1021 gctcataaca ccccttgttt gcctggcggc agtagcgcgg tggtcccacc tgaccccatg
     1081 ccgaactcag aagtgaaacg ccgtagcgcc gatggtagtg tggggactcc ccatgcgaga
     1141 gtagggaact gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
     1201 cccgggctaa ttatggggtg tcgcccttat tcgactctat agtgaagttc ctattctcta
     1261 gaaagtatag gaacttctga agtggggatt taaatgcggc cgcgctgagg gtttaatcgt
     1321 ctatgaggag actgttagtt ggatatcagt aatgagacga aaaagctcga aatgaatgga
     1381 tatattcttt ttgctactgg caactgttga atatttaatg ttaaaacaaa ctaactgagg
     1441 tatattcgta tctgtatgta cacatatact atatacagga aaagataagc aagagagagg
     1501 atatcaacta cgagagcgat cgattatata tcaaaagctg tccgctttgc cacccataat
     1561 cggcgcttag tttcggagtt caatcataat tctaccacct tacactcaac ttactcttta
     1621 actcctatag tataatatcg ccactgaccc catattaaaa aatttttttg ctcgatcttc
     1681 tatcctcttt aggttaattg tcgctgttat tgtctagatt ttttctcgga gatggcgcat
     1741 ctatttgccg tcaaaagatc ctctcatacc atattaagta aattgcctcc atttcttttt
     1801 cctcgggcag agaaactcgc aggcaacttg ctctcgaagt ggtcacgtTG GACATACATA
     1861 GTATACTCTG GTGATTGGGG TGGTTCGTCA ATGTGTAGAG CGTTGTGAGT AAGTTATTGA
     1921 GTTCAACGGG GCAAATCTTT ATATTGGGGA TAACTAAAGT CAATGCGAAC CATAGTTGAA
     1981 CTAACTTGGG GGAGAGcctg cataatcggc ctcacagagg gatcccgtta cccatctatg
     2041 ctgaagattt atcatactat tcctccgctc gtttcttttt tcagtgaggt gtgtcgtgaa
     2101 agaaaaccca caattaaact ttcaacaacc gggcgactag gaagagagta gtgggcgcgg
     2161 atgacgaagg ctaaggtcac ttcttcgttt cctttattgg ggtttccgtg tagccttccc
     2221 ctgaatagtg tgggacgttt tatgagaagc cgtaagaaat aggcaaattg agttatgaca
     2281 agtagacatg atgccgcagc cttgcctgac tttacgtctc cttcatgaat aagtttttct
     2341 atcgagttct tttccttttt tcgccttaat tagctcaatt aagcctgtcc tcactacttt
     2401 tctttttctt atcggctttg tgccacacct aaccttcgaa tgctgtttta ttccgttctt
     2461 acatgggatg gtaatgcctt ggcgagatta agacctcagc gcggccgcaa atttaaataa
     2521 aatgaagtga agttcctata ctttctagag aataggaact tctatagtga gtcgaataag
     2581 ggcgacacaa aatttattct aaatgcataa taaatactga taacatctta tagtttgtat
     2641 tatattttgt attatcgttg acatgtataa ttttgatatc aaaaactgat tttcccttta
     2701 ttattttcga gatttatttt cttaattctc tttaacaaac tagaaatatt gtatatacaa
     2761 aaaatcataa ataatagatg aatagtttaa ttataggtgt tcatcaatcg aaaaagcaac
     2821 gtatcttatt taaagtgcgt tgcttttttc tcatttataa ggttaaataa ttctcatata
     2881 tcaagcaaag tgacaggcgc ccttaaatat tctgacaaat gctctttccc taaactcccc
     2941 ccataaaaaa acccgccgaa gcgggttttt acgttatttg cggattaacg attactcgtt
     3001 atcagaaccg cccagggggc ccgagcttaa gactggccgt cgttttacaa cacagaaaga
     3061 gtttgtagaa acgcaaaaag gccatccgtc aggggccttc tgcttagttt gatgcctggc
     3121 agttccctac tctcgccttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     3181 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     3241 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     3301 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     3361 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     3421 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     3481 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     3541 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     3601 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     3661 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     3721 ttcttgaagt ggtgggctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     3781 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     3841 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     3901 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgacgcgcgc
     3961 gtaactcacg ttaagggatt ttggtcatga gcttgcgccg tcccgtcaag tcagcgtaat
     4021 gctctgcttt t
//