|DIFF||Parent commit||Current commit|
|Created at||Monday, 17 Jan 2022 3:38 PM||Monday, 17 Jan 2022 4:31 PM|
Bacterial two-hybrid vector designed to express a given polypeptide fused in frame at its N-terminal end with T25 fragment; p15 ori, KmR
Bacterial two-hybrid vector designed to express a given polypeptide fused in frame at its N-terminal end with TM domain 1 of E. coli oppB and T25 fragment, p15 ori, KmR The TM region was PCR amplified from pSTM25b with primer pair 1/2 cut with SalI/EcoRI and cloned into SalI/EcoRI digested pKT25
This plasmid was propagated using 10-beta strain: Δ(ara-leu) 7697 araD139 fhuA ΔlacX74 galK16 galE15 e14- ϕ80dlacZΔM15 recA1 relA1 endA1 nupG rpsL
Primers 1 and 2
|Next gen seq|
|Certification url or doi|
|Location||-80 freezer- Birgit Koch collection- Devonshire building|
|Obtained from||Birgit Koch created this plasmid from the parent plasmid pKT25|
|Assign as parent||No||No|
|Files||2496fa6050868c7314ab71357e4abb92501adeec-2496fa6050868c7314ab71357e4abb92501adeec-2496fa6050868c7314ab71357e4abb92501adeec-2496fa6050868c7314ab71357e4abb92501adeec-pKTM25.dna, 2496fa6050868c7314ab71357e4abb92501adeec-2496fa6050868c7314ab71357e4abb92501adeec-pKTM25.docx, 2496fa6050868c7314ab71357e4abb92501adeec-pKTM25-Gel-image.png|
|DIFF||Parent commit||Current commit|
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