Catalogue

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Here you can view all of the cells available to you in CellRepo, filtered by Species, Strain or Plasmid

Strain Name/Code Species DNA barcoded Has plasmid? Genotype Phenotype Author Organisation
This plasmid was propagated using 10-beta strain: Δ(ara-leu) 7697 araD139 fhuA ΔlacX74 galK16 galE15 e14-  ϕ80dlacZΔM15  recA1 relA1 endA1 nupG  rpsL  Plasmids This plasmid is based on pUTM18C (Ouellette et al., 2014): As pUT18C but designed to insert the TM domain 1 of E. coli oppB between the cloned polypeptide and the T18 fragment; ColE1 ori, AmpR. Ampicillin resistance JonathanT
This plasmid was propagated using 10-beta strain: Δ(ara-leu) 7697 araD139 fhuA ΔlacX74 galK16 galE15 e14-  ϕ80dlacZΔM15  recA1 relA1 endA1 nupG  rpsL  Plasmids This plasmid is based on pKT25 (Euromedex): Bacterial two-hybrid vector designed to express a given polypeptide fused in frame at its N-terminal end with T25 fragment; p15 ori, KmR Kanamycin resistant JonathanT
s369 Pseudomonas putida Δprophage1 Δprophage4 Δprophage3 Δprophage2 ΔTn7 ΔendA-1 ΔendA-2 ΔhsdRMS Δflagellum ΔTn4652 ΔrecA. BC6 (upstream of glmS gene) JonathanT
This plasmid was propagated using 10-beta strain: Δ(ara-leu) 7697 araD139 fhuA ΔlacX74 galK16 galE15 e14-  ϕ80dlacZΔM15  recA1 relA1 endA1 nupG  rpsL  Plasmids This plasmid is based on pKTM25 (this study): Bacterial two-hybrid vector designed to express a given polypeptide fused in frame at its N-terminal end with TM domain 1 of E. coli oppB and T25 fragment, p15 ori, KmR The TM region was PCR amplified from pSTM25 with primer pair 1/2 cut with SalI/EcoRI and cloned into SalI/EcoRI digested pKT25. Kanamycin resistant JonathanT
Plasmids This plasmid is based on pUT18C (Karimova et al 2001): Bacterial two-hybrid vector designed to express a given polypeptide fused in frame at its N-terminal end with T18 fragment; ColE1 ori, AmpR.  Ampicillin resistance JonathanT
This plasmid was propagated using 10-beta strain: Δ(ara-leu) 7697 araD139 fhuA ΔlacX74 galK16 galE15 e14-  ϕ80dlacZΔM15  recA1 relA1 endA1 nupG  rpsL  Plasmids Protein expression vector. AmpR (Novagen) Ampicillin resistance JonathanT
This plasmid was propagated using 10-beta strain: Δ(ara-leu) 7697 araD139 fhuA ΔlacX74 galK16 galE15 e14-  ϕ80dlacZΔM15  recA1 relA1 endA1 nupG  rpsL  Plasmids This plasmid is based on pKTM25 (this study): Bacterial two-hybrid vector designed to express a given polypeptide fused in frame at its N-terminal end with TM domain 1 of E. coli oppB and T25 fragment, p15 ori, KmR. The TM region was PCR amplified from pSTM25 with primer pair 1/2 cut with SalI/EcoRI and cloned into SalI/EcoRI digested pKT25 Kanamycin resistant JonathanT
Pseudomonas putida Elena Velázquez Centro Nacional de Biotecnología

EV118 (Elena's stock collection)

Pseudomonas putida Elena Velázquez Centro Nacional de Biotecnología
s361 Bacillus subtilis trpC2, BC659 (in between rpsD and tyrS genes) Trp(-) JonathanT
s191 Saccharomyces cerevisiae S288C-derivative laboratory strain: MATα his3Δ1 leu2Δ0 lys2Δ0 ura3Δ0 Auxotrophic for: histidine, leucine, lysine and uracil JonathanT
s239 Komagataella phaffii his4 Auxotrophic for histidine JonathanT
s366 Pseudomonas putida Δprophage1 Δprophage4 Δprophage3 Δprophage2 ΔTn7 ΔendA-1 ΔendA-2 ΔhsdRMS Δflagellum ΔTn4652 ΔrecA JonathanT

EV137 (Elena's stock collection)

Pseudomonas putida Elena Velázquez Centro Nacional de Biotecnología
This plasmid was propagated using 10-beta strain: Δ(ara-leu) 7697 araD139 fhuA ΔlacX74 galK16 galE15 e14-  ϕ80dlacZΔM15  recA1 relA1 endA1 nupG  rpsL  Plasmids This plasmid is based on pUT18MC (Ouellette et al., 2014): As pUT18C but designed to insert the TM domain 1 of E. coli oppB between the cloned polypeptide and the T18 fragment; ColE1 ori, AmpR. Ampicillin resistance JonathanT

EV138 (Elena's stock collection)

Pseudomonas putida Elena Velázquez Centro Nacional de Biotecnología
plasmid Nadia Rostami
This plasmid was propagated using 10-beta strain: Δ(ara-leu) 7697 araD139 fhuA ΔlacX74 galK16 galE15 e14-  ϕ80dlacZΔM15  recA1 relA1 endA1 nupG  rpsL  Plasmids This plasmid is based on pKT25 (Euromedex): Bacterial two-hybrid vector designed to express a given polypeptide fused in frame at its N-terminal end with T25 fragment; p15 ori, KmR Kanamycin resistant JonathanT
plasmid Nadia Rostami
s289 Bacillus subtilis trpC2, ZeoR-BC659 (in between rpsD and tyrS genes) Trp(-), Zeocin resistant JonathanT
s294 Bacillus subtilis trpC2, BC659 (in between rpsD and tyrS genes) Trp(-) JonathanT
s356 Bacillus subtilis trpC2, BC659 (in between rpsD and tyrS genes) Trp(-) JonathanT
BW25113 - BC (CRISPR) Escherichia coli lacI+ rrnBT14 ΔlacZWJ16 hsdR514 ΔaraBADAH33 ΔrhaBADLD78BC383 JonathanT
168 - amyE GFP-cat (BC207) Bacillus subtilis trpC2, amyE::GFP-cat, BC207-zeoR Trp(-), Chloramphenicol resistant, Zeocin resistant JonathanT
This plasmid was propagated using 10-beta strain: Δ(ara-leu) 7697 araD139 fhuA ΔlacX74 galK16 galE15 e14-  ϕ80dlacZΔM15  recA1 relA1 endA1 nupG  rpsL  Plasmids This plasmid is  based on pUT18C (Karimova et al 2001): Bacterial two-hybrid vector designed to express a given polypeptide fused in frame at its N-terminal end with T18 fragment; ColE1 ori, AmpR. Ampicillin resistance JonathanT
s268 Streptomyces albidoflavus BC4 (inserted in between XNR_3871 and XNR_3872) JonathanT
s316 Escherichia coli rrnB3 ΔlacZ4787 hsdR514 Δ(araBAD)567 Δ(rhaBAD)568 rph-1. BC383 (in between pyrH and frr) JonathanT
s279 Escherichia coli rrnB3 ΔlacZ4787 hsdR514 Δ(araBAD)567 Δ(rhaBAD)568 rph-1. CmR-BC383 (in between pyrH and frr) Chloramphenicol resistance JonathanT
s284 Escherichia coli rrnB3 ΔlacZ4787 hsdR514 Δ(araBAD)567 Δ(rhaBAD)568 rph-1. BC383 (in between pyrH and frr) JonathanT
s349 Saccharomyces cerevisiae S288C-derivative laboratory strain: MATα his3Δ1 leu2Δ0 lys2Δ0 ura3Δ0. KanMX-BC2 (inserted between NCA3 and ASF1) Auxotrophic for: histidine, leucine, lysine and uracil. Kanamycin (G418) resistant JonathanT
s392 Saccharomyces cerevisiae S288C-derivative laboratory strain: MATα his3Δ1 leu2Δ0 lys2Δ0 ura3Δ0. BC2 (inserted between NCA3 and ASF1) Auxotrophic for: histidine, leucine, lysine and uracil.  JonathanT
s337 Saccharomyces cerevisiae S288C-derivative laboratory strain: MATα his3Δ1 leu2Δ0 lys2Δ0 ura3Δ0. BC14 (inserted between NCA3 and ASF1) Auxotrophic for: histidine, leucine, lysine and uracil.  JonathanT
168 (BC659) Bacillus subtilis trpC2Barcode: BC659 Trp(-) JonathanT
168 - amyE GFP-cat Bacillus subtilis trpC2, amyE::GFP-cat Trp(-), Chloramphenicol resistant JonathanT

HM1

Escherichia coli F-lambda-rph- INV (rrnD, rrnE)  ΔlacI JonathanT
BW25113 Escherichia coli lacI+ rrnBT14 ΔlacZWJ16 hsdR514 ΔaraBADAH33 ΔrhaBADLD78 JonathanT
BW25113 Escherichia coli rrnB3 ΔlacZ4787 hsdR514 Δ(araBAD)567 Δ(rhaBAD)568 rph-1. JonathanT

HM2

Escherichia coli F-lambda-rph- INV (rrnD, rrnE)  ΔlacI ΔlysA Lysine auxotrophic JonathanT
s216 Streptomyces albidoflavus JonathanT
Plasmids pET22b: Protein expression vector. AmpR (Novagen) Ampicillin resistance JonathanT

HM3

Escherichia coli F-lambda-rph- INV (rrnD, rrnE)  ΔlacI ΔlysA ΔmetA Lysine, methionine auxotrophic JonathanT

HM4

Escherichia coli F-lambda-rph- INV (rrnD, rrnE)  ΔlacI ΔlysA ΔmetA ΔthrBC Lysine, methionine and threonine auxotrophic JonathanT
s256 Komagataella phaffii his4, ZeoR-BC10 (downstream of AOX1 gene) Auxotrophic for histidine, Zeocin resistant JonathanT

W3110

Escherichia coli F-lambda-rph- INV (rrnD, rrnE) JonathanT
BW25113 - BC Escherichia coli lacI+ rrnBT14 ΔlacZWJ16 hsdR514 ΔaraBADAH33 ΔrhaBADLD78BC383 JonathanT
s142 Bacillus subtilis trpC2 Trp(-) JonathanT
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