Catalogue

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Here you can view all of the cells available to you in CellRepo, filtered by Strain or Plasmid

Strain Name/Code Species DNA barcoded Has plasmid? Genotype Phenotype Author Organisation
This plasmid was propagated using 10-beta strain: Δ(ara-leu) 7697 araD139 fhuA ΔlacX74 galK16 galE15 e14-  ϕ80dlacZΔM15  recA1 relA1 endA1 nupG  rpsL  Plasmids This plasmid is based on pUTM18C (Ouellette et al., 2014): As pUT18C but designed to insert the TM domain 1 of E. coli oppB between the cloned polypeptide and the T18 fragment; ColE1 ori, AmpR. Ampicillin resistance JonathanT Newcastle University
This plasmid was propagated using 10-beta strain: Δ(ara-leu) 7697 araD139 fhuA ΔlacX74 galK16 galE15 e14-  ϕ80dlacZΔM15  recA1 relA1 endA1 nupG  rpsL  Plasmids This plasmid is based on pKT25 (Euromedex): Bacterial two-hybrid vector designed to express a given polypeptide fused in frame at its N-terminal end with T25 fragment; p15 ori, KmR Kanamycin resistant JonathanT Newcastle University
s369 Pseudomonas putida Δprophage1 Δprophage4 Δprophage3 Δprophage2 ΔTn7 ΔendA-1 ΔendA-2 ΔhsdRMS Δflagellum ΔTn4652 ΔrecA. BC6 (upstream of glmS gene) JonathanT Newcastle University
This plasmid was propagated using 10-beta strain: Δ(ara-leu) 7697 araD139 fhuA ΔlacX74 galK16 galE15 e14-  ϕ80dlacZΔM15  recA1 relA1 endA1 nupG  rpsL  Plasmids This plasmid is based on pKTM25 (this study): Bacterial two-hybrid vector designed to express a given polypeptide fused in frame at its N-terminal end with TM domain 1 of E. coli oppB and T25 fragment, p15 ori, KmR The TM region was PCR amplified from pSTM25 with primer pair 1/2 cut with SalI/EcoRI and cloned into SalI/EcoRI digested pKT25. Kanamycin resistant JonathanT Newcastle University
Plasmids This plasmid is based on pUT18C (Karimova et al 2001): Bacterial two-hybrid vector designed to express a given polypeptide fused in frame at its N-terminal end with T18 fragment; ColE1 ori, AmpR.  Ampicillin resistance JonathanT Newcastle University
This plasmid was propagated using 10-beta strain: Δ(ara-leu) 7697 araD139 fhuA ΔlacX74 galK16 galE15 e14-  ϕ80dlacZΔM15  recA1 relA1 endA1 nupG  rpsL  Plasmids Protein expression vector. AmpR (Novagen) Ampicillin resistance JonathanT Newcastle University
This plasmid was propagated using 10-beta strain: Δ(ara-leu) 7697 araD139 fhuA ΔlacX74 galK16 galE15 e14-  ϕ80dlacZΔM15  recA1 relA1 endA1 nupG  rpsL  Plasmids This plasmid is based on pKTM25 (this study): Bacterial two-hybrid vector designed to express a given polypeptide fused in frame at its N-terminal end with TM domain 1 of E. coli oppB and T25 fragment, p15 ori, KmR. The TM region was PCR amplified from pSTM25 with primer pair 1/2 cut with SalI/EcoRI and cloned into SalI/EcoRI digested pKT25 Kanamycin resistant JonathanT Newcastle University
Pseudomonas putida Elena Velázquez National Center for Biotechnology

EV118 (Elena's stock collection)

Pseudomonas putida Elena Velázquez National Center for Biotechnology
s361 Bacillus subtilis trpC2, BC659 (in between rpsD and tyrS genes) Trp(-) JonathanT Newcastle University
s191 Saccharomyces cerevisiae S288C-derivative laboratory strain: MATα his3Δ1 leu2Δ0 lys2Δ0 ura3Δ0 Auxotrophic for: histidine, leucine, lysine and uracil JonathanT Newcastle University
s239 Komagataella phaffii his4 Auxotrophic for histidine JonathanT Newcastle University
s366 Pseudomonas putida Δprophage1 Δprophage4 Δprophage3 Δprophage2 ΔTn7 ΔendA-1 ΔendA-2 ΔhsdRMS Δflagellum ΔTn4652 ΔrecA JonathanT Newcastle University

EV137 (Elena's stock collection)

Pseudomonas putida Elena Velázquez National Center for Biotechnology
This plasmid was propagated using 10-beta strain: Δ(ara-leu) 7697 araD139 fhuA ΔlacX74 galK16 galE15 e14-  ϕ80dlacZΔM15  recA1 relA1 endA1 nupG  rpsL  Plasmids This plasmid is based on pUT18MC (Ouellette et al., 2014): As pUT18C but designed to insert the TM domain 1 of E. coli oppB between the cloned polypeptide and the T18 fragment; ColE1 ori, AmpR. Ampicillin resistance JonathanT Newcastle University

EV138 (Elena's stock collection)

Pseudomonas putida Elena Velázquez National Center for Biotechnology
plasmid Nadia Rostami GitLife Biotech
This plasmid was propagated using 10-beta strain: Δ(ara-leu) 7697 araD139 fhuA ΔlacX74 galK16 galE15 e14-  ϕ80dlacZΔM15  recA1 relA1 endA1 nupG  rpsL  Plasmids This plasmid is based on pKT25 (Euromedex): Bacterial two-hybrid vector designed to express a given polypeptide fused in frame at its N-terminal end with T25 fragment; p15 ori, KmR Kanamycin resistant JonathanT Newcastle University
plasmid Nadia Rostami GitLife Biotech
s289 Bacillus subtilis trpC2, ZeoR-BC659 (in between rpsD and tyrS genes) Trp(-), Zeocin resistant JonathanT Newcastle University
s294 Bacillus subtilis trpC2, BC659 (in between rpsD and tyrS genes) Trp(-) JonathanT Newcastle University
s356 Bacillus subtilis trpC2, BC659 (in between rpsD and tyrS genes) Trp(-) JonathanT Newcastle University
BW25113 - BC (CRISPR) Escherichia coli lacI+ rrnBT14 ΔlacZWJ16 hsdR514 ΔaraBADAH33 ΔrhaBADLD78BC383 JonathanT Newcastle University
168 - amyE GFP-cat (BC207) Bacillus subtilis trpC2, amyE::GFP-cat, BC207-zeoR Trp(-), Chloramphenicol resistant, Zeocin resistant JonathanT Newcastle University
This plasmid was propagated using 10-beta strain: Δ(ara-leu) 7697 araD139 fhuA ΔlacX74 galK16 galE15 e14-  ϕ80dlacZΔM15  recA1 relA1 endA1 nupG  rpsL  Plasmids This plasmid is  based on pUT18C (Karimova et al 2001): Bacterial two-hybrid vector designed to express a given polypeptide fused in frame at its N-terminal end with T18 fragment; ColE1 ori, AmpR. Ampicillin resistance JonathanT Newcastle University
s268 Streptomyces albidoflavus BC4 (inserted in between XNR_3871 and XNR_3872) JonathanT Newcastle University
s316 Escherichia coli rrnB3 ΔlacZ4787 hsdR514 Δ(araBAD)567 Δ(rhaBAD)568 rph-1. BC383 (in between pyrH and frr) JonathanT Newcastle University
s279 Escherichia coli rrnB3 ΔlacZ4787 hsdR514 Δ(araBAD)567 Δ(rhaBAD)568 rph-1. CmR-BC383 (in between pyrH and frr) Chloramphenicol resistance JonathanT Newcastle University
s284 Escherichia coli rrnB3 ΔlacZ4787 hsdR514 Δ(araBAD)567 Δ(rhaBAD)568 rph-1. BC383 (in between pyrH and frr) JonathanT Newcastle University
s349 Saccharomyces cerevisiae S288C-derivative laboratory strain: MATα his3Δ1 leu2Δ0 lys2Δ0 ura3Δ0. KanMX-BC2 (inserted between NCA3 and ASF1) Auxotrophic for: histidine, leucine, lysine and uracil. Kanamycin (G418) resistant JonathanT Newcastle University
s392 Saccharomyces cerevisiae S288C-derivative laboratory strain: MATα his3Δ1 leu2Δ0 lys2Δ0 ura3Δ0. BC2 (inserted between NCA3 and ASF1) Auxotrophic for: histidine, leucine, lysine and uracil.  JonathanT Newcastle University
s337 Saccharomyces cerevisiae S288C-derivative laboratory strain: MATα his3Δ1 leu2Δ0 lys2Δ0 ura3Δ0. BC14 (inserted between NCA3 and ASF1) Auxotrophic for: histidine, leucine, lysine and uracil.  JonathanT Newcastle University
168 (BC659) Bacillus subtilis trpC2Barcode: BC659 Trp(-) JonathanT Newcastle University
168 - amyE GFP-cat Bacillus subtilis trpC2, amyE::GFP-cat Trp(-), Chloramphenicol resistant JonathanT Newcastle University

HM1

Escherichia coli F-lambda-rph- INV (rrnD, rrnE)  ΔlacI JonathanT Newcastle University
BW25113 Escherichia coli lacI+ rrnBT14 ΔlacZWJ16 hsdR514 ΔaraBADAH33 ΔrhaBADLD78 JonathanT Newcastle University
BW25113 Escherichia coli rrnB3 ΔlacZ4787 hsdR514 Δ(araBAD)567 Δ(rhaBAD)568 rph-1. JonathanT Newcastle University

HM2

Escherichia coli F-lambda-rph- INV (rrnD, rrnE)  ΔlacI ΔlysA Lysine auxotrophic JonathanT Newcastle University
s216 Streptomyces albidoflavus JonathanT Newcastle University
Plasmids pET22b: Protein expression vector. AmpR (Novagen) Ampicillin resistance JonathanT Newcastle University

HM3

Escherichia coli F-lambda-rph- INV (rrnD, rrnE)  ΔlacI ΔlysA ΔmetA Lysine, methionine auxotrophic JonathanT Newcastle University

HM4

Escherichia coli F-lambda-rph- INV (rrnD, rrnE)  ΔlacI ΔlysA ΔmetA ΔthrBC Lysine, methionine and threonine auxotrophic JonathanT Newcastle University
s256 Komagataella phaffii his4, ZeoR-BC10 (downstream of AOX1 gene) Auxotrophic for histidine, Zeocin resistant JonathanT Newcastle University

W3110

Escherichia coli F-lambda-rph- INV (rrnD, rrnE) JonathanT Newcastle University
BW25113 - BC Escherichia coli lacI+ rrnBT14 ΔlacZWJ16 hsdR514 ΔaraBADAH33 ΔrhaBADLD78BC383 JonathanT Newcastle University
s142 Bacillus subtilis trpC2 Trp(-) JonathanT Newcastle University
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