Catalogue

Image depicting a species

Here you can view all of the cells available to you in CellRepo, filtered by Strain or Plasmid

Strain Name/Code Bioasset DNA barcoded Has plasmid? Genotype Phenotype Author Organisation
s361 Bacillus subtilis trpC2, BC659 (in between rpsD and tyrS genes) Trp(-) JonathanT Newcastle University
This plasmid was propagated using 10-beta strain: Δ(ara-leu) 7697 araD139 fhuA ΔlacX74 galK16 galE15 e14-  ϕ80dlacZΔM15  recA1 relA1 endA1 nupG  rpsL  Plasmids This plasmid is based on pKT25 (Euromedex): Bacterial two-hybrid vector designed to express a given polypeptide fused in frame at its N-terminal end with T25 fragment; p15 ori, KmR Kanamycin resistant JonathanT Newcastle University
BW25113 - BC Escherichia coli lacI+ rrnBT14 ΔlacZWJ16 hsdR514 ΔaraBADAH33 ΔrhaBADLD78BC383 JonathanT Newcastle University
This plasmid was propagated using 10-beta strain: Δ(ara-leu) 7697 araD139 fhuA ΔlacX74 galK16 galE15 e14-  ϕ80dlacZΔM15  recA1 relA1 endA1 nupG  rpsL  Plasmids This plasmid is based on pKTM25 (this study): Bacterial two-hybrid vector designed to express a given polypeptide fused in frame at its N-terminal end with TM domain 1 of E. coli oppB and T25 fragment, p15 ori, KmR. The TM region was PCR amplified from pSTM25 with primer pair 1/2 cut with SalI/EcoRI and cloned into SalI/EcoRI digested pKT25 Kanamycin resistant JonathanT Newcastle University
s289 Bacillus subtilis trpC2, ZeoR-BC659 (in between rpsD and tyrS genes) Trp(-), Zeocin resistant JonathanT Newcastle University
This plasmid was propagated using 10-beta strain: Δ(ara-leu) 7697 araD139 fhuA ΔlacX74 galK16 galE15 e14-  ϕ80dlacZΔM15  recA1 relA1 endA1 nupG  rpsL  Plasmids This plasmid is based on pUTM18C (Ouellette et al., 2014): As pUT18C but designed to insert the TM domain 1 of E. coli oppB between the cloned polypeptide and the T18 fragment; ColE1 ori, AmpR. Ampicillin resistance JonathanT Newcastle University
Plasmids pET22b: Protein expression vector. AmpR (Novagen) Ampicillin resistance JonathanT Newcastle University
This plasmid was propagated using 10-beta strain: Δ(ara-leu) 7697 araD139 fhuA ΔlacX74 galK16 galE15 e14-  ϕ80dlacZΔM15  recA1 relA1 endA1 nupG  rpsL  Plasmids This plasmid is based on pKTM25 (this study): Bacterial two-hybrid vector designed to express a given polypeptide fused in frame at its N-terminal end with TM domain 1 of E. coli oppB and T25 fragment, p15 ori, KmR The TM region was PCR amplified from pSTM25 with primer pair 1/2 cut with SalI/EcoRI and cloned into SalI/EcoRI digested pKT25. Kanamycin resistant JonathanT Newcastle University
Plasmids This plasmid is based on pUT18C (Karimova et al 2001): Bacterial two-hybrid vector designed to express a given polypeptide fused in frame at its N-terminal end with T18 fragment; ColE1 ori, AmpR.  Ampicillin resistance JonathanT Newcastle University
This plasmid was propagated using 10-beta strain: Δ(ara-leu) 7697 araD139 fhuA ΔlacX74 galK16 galE15 e14-  ϕ80dlacZΔM15  recA1 relA1 endA1 nupG  rpsL  Plasmids This plasmid is based on pUT18MC (Ouellette et al., 2014): As pUT18C but designed to insert the TM domain 1 of E. coli oppB between the cloned polypeptide and the T18 fragment; ColE1 ori, AmpR. Ampicillin resistance JonathanT Newcastle University
Plasmid Name Target Species Propagation Species DNA Barcoded Insert Origin of Replication Promoters Terminators Antibiotic Resistance Author Organisation
168 - Wild type JonathanT Newcastle University
168 - ZeoR - BC659 JonathanT Newcastle University
168 - BC659 JonathanT Newcastle University
168 - BC659 JonathanT Newcastle University
168 - BC659 JonathanT Newcastle University
EM383 - wild type JonathanT Newcastle University
EM383 - BC6 JonathanT Newcastle University
J1074 - wild type JonathanT Newcastle University
J1074 - BC4 JonathanT Newcastle University
GS115 - wild type JonathanT Newcastle University