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JonathanT

Last updated: Jun 05, 2020

168 strain (parent strain of this repo)

This is the initial strain in this repository obtained from the shown reference. The attached file describes the transformation protocol used.

This strain was barcoded with barcode BC659 following the Cre-Lox protocol in between the ybxH and ybxI genes (zeocin resistance removed).

The genome of this strain is attached.

Draft
Barcode
v1 dcabd7

dcabd7cccc09420682210d4384131e65b2c2242df

v1 CTTACT

CTTACTATGCCACATAGTCTAACTACTTTGCGTAGGTAATAGAGCAACGAGTAGGGTTATCGTCTTTCATACTCGGTTGGCGATTATACAAACTCCAATGGTTTGATTGGTGTTTCTCATACG

Whole genome sequence

Burkholder, P. R., & Giles, N. H. (1947). INDUCED BIOCHEMICAL MUTATIONS IN BACILLUS SUBTILIS. American Journal of Botany, 34(6), 345–348. doi:10.1002/j.1537-2197.1947.tb12999.x 

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Strain data

Bacillus subtilis

ATCC 6051

168 (BC659)

The initial aliquot of 168 cells was obtained from our strain collection in Devonshire Building.

trpC2
Barcode: BC659

Trp(-)

Devonshire Building. ICOS -80 room. Freezer 2. Box 2.

May 16, 2019