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Catalogue
Here you can view all of the cells available to you in CellRepo, filtered by Strain or Plasmid
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| Type | Name/Code | Bioasset | DNA Barcoded | Insert/Plasmid | Origin of Replication/Genotype | Promoters/Phenotype | Terminators | Antibiotic Resistance | Author | Organisation |
|---|---|---|---|---|---|---|---|---|---|---|
| Strain | NCYC 2543 HOC1tr-2 | Komagataella phaffii | HOC1 Truncation | Ben Hastings | ||||||
| Strain | NCYC-2543 HOC1tr-1 | Komagataella phaffii | HOC1 Truncation | Ben Hastings | ||||||
| Strain | NCYC 2543 | Komagataella phaffii | Ben Hastings | |||||||
| Strain | pKTM25 | plasmid | Nadia Rostami | |||||||
| Strain | pKT25 | plasmid | Nadia Rostami | |||||||
| Strain | EV138 (Elena's stock collection) | Pseudomonas putida | Elena Velázquez | National Center for Biotechnology | ||||||
| Strain | EV137 (Elena's stock collection) | Pseudomonas putida | Elena Velázquez | National Center for Biotechnology | ||||||
| Strain | EV118 (Elena's stock collection) | Pseudomonas putida | Elena Velázquez | National Center for Biotechnology | ||||||
| Strain | P. putida KT2440 | Pseudomonas putida | Elena Velázquez | National Center for Biotechnology | ||||||
| Strain | HM4 | Escherichia coli | F-lambda-rph- INV (rrnD, rrnE) ΔlacI ΔlysA ΔmetA ΔthrBC | Lysine, methionine and threonine auxotrophic | JonathanT | Newcastle University | ||||
| Strain | HM3 | Escherichia coli | F-lambda-rph- INV (rrnD, rrnE) ΔlacI ΔlysA ΔmetA | Lysine, methionine auxotrophic | JonathanT | Newcastle University | ||||
| Strain | HM2 | Escherichia coli | F-lambda-rph- INV (rrnD, rrnE) ΔlacI ΔlysA | Lysine auxotrophic | JonathanT | Newcastle University | ||||
| Strain | HM1 | Escherichia coli | F-lambda-rph- INV (rrnD, rrnE) ΔlacI | JonathanT | Newcastle University | |||||
| Strain | W3110 | Escherichia coli | F-lambda-rph- INV (rrnD, rrnE) | JonathanT | Newcastle University | |||||
| Strain | s337 | Saccharomyces cerevisiae | S288C-derivative laboratory strain: MATα his3Δ1 leu2Δ0 lys2Δ0 ura3Δ0. BC14 (inserted between NCA3 and ASF1) | Auxotrophic for: histidine, leucine, lysine and uracil. | JonathanT | Newcastle University | ||||
| Strain | s392 | Saccharomyces cerevisiae | S288C-derivative laboratory strain: MATα his3Δ1 leu2Δ0 lys2Δ0 ura3Δ0. BC2 (inserted between NCA3 and ASF1) | Auxotrophic for: histidine, leucine, lysine and uracil. | JonathanT | Newcastle University | ||||
| Strain | s349 | Saccharomyces cerevisiae | S288C-derivative laboratory strain: MATα his3Δ1 leu2Δ0 lys2Δ0 ura3Δ0. KanMX-BC2 (inserted between NCA3 and ASF1) | Auxotrophic for: histidine, leucine, lysine and uracil. Kanamycin (G418) resistant | JonathanT | Newcastle University | ||||
| Strain | s191 | Saccharomyces cerevisiae | S288C-derivative laboratory strain: MATα his3Δ1 leu2Δ0 lys2Δ0 ura3Δ0 | Auxotrophic for: histidine, leucine, lysine and uracil | JonathanT | Newcastle University | ||||
| Strain | s256 | Komagataella phaffii | his4, ZeoR-BC10 (downstream of AOX1 gene) | Auxotrophic for histidine, Zeocin resistant | JonathanT | Newcastle University | ||||
| Strain | s239 | Komagataella phaffii | his4 | Auxotrophic for histidine | JonathanT | Newcastle University | ||||
| Strain | s268 | Streptomyces albidoflavus | BC4 (inserted in between XNR_3871 and XNR_3872) | JonathanT | Newcastle University | |||||
| Strain | s216 | Streptomyces albidoflavus | JonathanT | Newcastle University | ||||||
| Strain | s369 | Pseudomonas putida | Δprophage1 Δprophage4 Δprophage3 Δprophage2 ΔTn7 ΔendA-1 ΔendA-2 ΔhsdRMS Δflagellum ΔTn4652 ΔrecA. BC6 (upstream of glmS gene) | JonathanT | Newcastle University | |||||
| Strain | s366 | Pseudomonas putida | Δprophage1 Δprophage4 Δprophage3 Δprophage2 ΔTn7 ΔendA-1 ΔendA-2 ΔhsdRMS Δflagellum ΔTn4652 ΔrecA | JonathanT | Newcastle University | |||||
| Strain | s356 | Bacillus subtilis | trpC2, BC659 (in between rpsD and tyrS genes) | Trp(-) | JonathanT | Newcastle University | ||||
| Strain | s361 | Bacillus subtilis | trpC2, BC659 (in between rpsD and tyrS genes) | Trp(-) | JonathanT | Newcastle University | ||||
| Strain | s294 | Bacillus subtilis | trpC2, BC659 (in between rpsD and tyrS genes) | Trp(-) | JonathanT | Newcastle University | ||||
| Strain | s289 | Bacillus subtilis | trpC2, ZeoR-BC659 (in between rpsD and tyrS genes) | Trp(-), Zeocin resistant | JonathanT | Newcastle University | ||||
| Strain | s142 | Bacillus subtilis | trpC2 | Trp(-) | JonathanT | Newcastle University | ||||
| Strain | s284 | Escherichia coli | rrnB3 ΔlacZ4787 hsdR514 Δ(araBAD)567 Δ(rhaBAD)568 rph-1. BC383 (in between pyrH and frr) | JonathanT | Newcastle University | |||||
| Strain | s279 | Escherichia coli | rrnB3 ΔlacZ4787 hsdR514 Δ(araBAD)567 Δ(rhaBAD)568 rph-1. CmR-BC383 (in between pyrH and frr) | Chloramphenicol resistance | JonathanT | Newcastle University | ||||
| Strain | s316 | Escherichia coli | rrnB3 ΔlacZ4787 hsdR514 Δ(araBAD)567 Δ(rhaBAD)568 rph-1. BC383 (in between pyrH and frr) | JonathanT | Newcastle University | |||||
| Strain | BW25113 | Escherichia coli | rrnB3 ΔlacZ4787 hsdR514 Δ(araBAD)567 Δ(rhaBAD)568 rph-1. | JonathanT | Newcastle University | |||||
| Strain | This plasmid was propagated using 10-beta strain: Δ(ara-leu) 7697 araD139 fhuA ΔlacX74 galK16 galE15 e14- ϕ80dlacZΔM15 recA1 relA1 endA1 nupG rpsL | Plasmids | Protein expression vector. AmpR (Novagen) | Ampicillin resistance | JonathanT | Newcastle University | ||||
| Strain | This plasmid was propagated using 10-beta strain: Δ(ara-leu) 7697 araD139 fhuA ΔlacX74 galK16 galE15 e14- ϕ80dlacZΔM15 recA1 relA1 endA1 nupG rpsL | Plasmids | This plasmid is based on pUT18MC (Ouellette et al., 2014): As pUT18C but designed to insert the TM domain 1 of E. coli oppB between the cloned polypeptide and the T18 fragment; ColE1 ori, AmpR. | Ampicillin resistance | JonathanT | Newcastle University | ||||
| Strain | Plasmids | This plasmid is based on pUT18C (Karimova et al 2001): Bacterial two-hybrid vector designed to express a given polypeptide fused in frame at its N-terminal end with T18 fragment; ColE1 ori, AmpR. | Ampicillin resistance | JonathanT | Newcastle University | |||||
| Strain | This plasmid was propagated using 10-beta strain: Δ(ara-leu) 7697 araD139 fhuA ΔlacX74 galK16 galE15 e14- ϕ80dlacZΔM15 recA1 relA1 endA1 nupG rpsL | Plasmids | This plasmid is based on pKTM25 (this study): Bacterial two-hybrid vector designed to express a given polypeptide fused in frame at its N-terminal end with TM domain 1 of E. coli oppB and T25 fragment, p15 ori, KmR The TM region was PCR amplified from pSTM25 with primer pair 1/2 cut with SalI/EcoRI and cloned into SalI/EcoRI digested pKT25. | Kanamycin resistant | JonathanT | Newcastle University | ||||
| Strain | This plasmid was propagated using 10-beta strain: Δ(ara-leu) 7697 araD139 fhuA ΔlacX74 galK16 galE15 e14- ϕ80dlacZΔM15 recA1 relA1 endA1 nupG rpsL | Plasmids | This plasmid is based on pKT25 (Euromedex): Bacterial two-hybrid vector designed to express a given polypeptide fused in frame at its N-terminal end with T25 fragment; p15 ori, KmR | Kanamycin resistant | JonathanT | Newcastle University | ||||
| Strain | Plasmids | pET22b: Protein expression vector. AmpR (Novagen) | Ampicillin resistance | JonathanT | Newcastle University | |||||
| Strain | This plasmid was propagated using 10-beta strain: Δ(ara-leu) 7697 araD139 fhuA ΔlacX74 galK16 galE15 e14- ϕ80dlacZΔM15 recA1 relA1 endA1 nupG rpsL | Plasmids | This plasmid is based on pUTM18C (Ouellette et al., 2014): As pUT18C but designed to insert the TM domain 1 of E. coli oppB between the cloned polypeptide and the T18 fragment; ColE1 ori, AmpR. | Ampicillin resistance | JonathanT | Newcastle University | ||||
| Strain | This plasmid was propagated using 10-beta strain: Δ(ara-leu) 7697 araD139 fhuA ΔlacX74 galK16 galE15 e14- ϕ80dlacZΔM15 recA1 relA1 endA1 nupG rpsL | Plasmids | This plasmid is based on pUT18C (Karimova et al 2001): Bacterial two-hybrid vector designed to express a given polypeptide fused in frame at its N-terminal end with T18 fragment; ColE1 ori, AmpR. | Ampicillin resistance | JonathanT | Newcastle University | ||||
| Strain | This plasmid was propagated using 10-beta strain: Δ(ara-leu) 7697 araD139 fhuA ΔlacX74 galK16 galE15 e14- ϕ80dlacZΔM15 recA1 relA1 endA1 nupG rpsL | Plasmids | This plasmid is based on pKTM25 (this study): Bacterial two-hybrid vector designed to express a given polypeptide fused in frame at its N-terminal end with TM domain 1 of E. coli oppB and T25 fragment, p15 ori, KmR. The TM region was PCR amplified from pSTM25 with primer pair 1/2 cut with SalI/EcoRI and cloned into SalI/EcoRI digested pKT25 | Kanamycin resistant | JonathanT | Newcastle University | ||||
| Strain | This plasmid was propagated using 10-beta strain: Δ(ara-leu) 7697 araD139 fhuA ΔlacX74 galK16 galE15 e14- ϕ80dlacZΔM15 recA1 relA1 endA1 nupG rpsL | Plasmids | This plasmid is based on pKT25 (Euromedex): Bacterial two-hybrid vector designed to express a given polypeptide fused in frame at its N-terminal end with T25 fragment; p15 ori, KmR | Kanamycin resistant | JonathanT | Newcastle University | ||||
| Strain | BW25113 - BC (CRISPR) | Escherichia coli | lacI+ rrnBT14 ΔlacZWJ16 hsdR514 ΔaraBADAH33 ΔrhaBADLD78BC383 | JonathanT | Newcastle University | |||||
| Strain | BW25113 - BC | Escherichia coli | lacI+ rrnBT14 ΔlacZWJ16 hsdR514 ΔaraBADAH33 ΔrhaBADLD78BC383 | JonathanT | Newcastle University | |||||
| Strain | BW25113 | Escherichia coli | lacI+ rrnBT14 ΔlacZWJ16 hsdR514 ΔaraBADAH33 ΔrhaBADLD78 | JonathanT | Newcastle University | |||||
| Strain | 168 - amyE GFP-cat (BC207) | Bacillus subtilis | trpC2, amyE::GFP-cat, BC207-zeoR | Trp(-), Chloramphenicol resistant, Zeocin resistant | JonathanT | Newcastle University | ||||
| Strain | 168 - amyE GFP-cat | Bacillus subtilis | trpC2, amyE::GFP-cat | Trp(-), Chloramphenicol resistant | JonathanT | Newcastle University | ||||
| Strain | 168 (BC659) | Bacillus subtilis | trpC2Barcode: BC659 | Trp(-) | JonathanT | Newcastle University |
