BW25113 - BC383 (CRISPR)

This is the barcoded version of BW25113 using CRISPR as barcoding method (Li et al. 2015). The attached plasmid contains the gRNA and the repair template.

Draft

Whole genome sequence

Sequence files

85e52b2b-f55c-4be6-8a5f-055938f003fbpEC-CRISPR-Vector-beaf9f3d482d.gb

Commit files

References

Li Y, Lin Z, Huang C, et al. Metabolic engineering of Escherichia coli using CRISPR-Cas9 meditated genome editing. Metab Eng. 2015;31:13‐21. doi:10.1016/j.ymben.2015.06.006

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Strain data

Barcode

v1.0 8328b5

8328b553bfd349f098d19ce9d95e05d6b2c0ad6eb

v1.0 GTCGTG

Genosignature: GTCGTGTCTGTCAATATCGGGGATAATAAATGTAGATAATGATTTCGTCAAGTCCTTACCCCGACTATACAACAGCAATCCATAGTAATCTGTGCGAATGGTTTAGTAGGACATAACACAAAT

Show GC content diagram

BW25113 - BC383 (CRISPR)

Strain data

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