Elena Velázquez

Last updated: Sep 01, 2021

P. putida KT2440 - B3

P. putida KT2440 (pSEVA421-Cas9tr // pSEVA6511-GIIi(B3)-94a) was barcoded with barcode #3 (TGGACATACATAGTATACTCTGGTG corresponds to the universal region present in all barcodes). Ll.LtrB intron delivery was used to carry out the insertion of the barcode and CRISPR/Cas9-mediated counterselection was implemented to facilitate the procedure.

Digital Barcode (Version 1.0)


Physical Barcode (Version 1.0)

Velázaquez E., et al. Targetron-assisted delivery of exogenous DNA sequences into Pseudomonas putida through CRISPR-aided counterselection (in preparation)

Strain data

Pseudomonas putida

ATCC 12633

EV138 (Elena's stock collection)

Elena Velázquez

Centro Nacional Biotecnología (CNB-CSIC), Madrid (Spain). Elena Velázquez Stock collection