P. putida KT2440 - B3

P. putida KT2440 (pSEVA421-Cas9tr // pSEVA6511-GIIi(B3)-94a) was barcoded with barcode #3 (TGGACATACATAGTATACTCTGGTG corresponds to the universal region present in all barcodes). Ll.LtrB intron delivery was used to carry out the insertion of the barcode and CRISPR/Cas9-mediated counterselection was implemented to facilitate the procedure.

Draft

Sequence files

Commit files

References

Velázaquez E., et al. Targetron-assisted delivery of exogenous DNA sequences into Pseudomonas putida through CRISPR-aided counterselection (in preparation)

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Strain data

Barcode

v1.0 de28be

de28be3dbcdc481f900b14ba6160d1a6b2ccc42cc

v1.0 TGGACA

Genosignature: TGGACATACATAGTATACTCTGGTGTTGAAGTTTCAAGGTTTTATCCGTAGGTTCAACTGCGTTGGGAGTGGGCAAGGGGGAATGGGCATAGATATAAAAACAATGGGGTTAGTAGGTTATCCATAGACTTGACAAGTTTGCCTTTG

Show GC content diagram

Species

Pseudomonas putida

P. putida KT2440 - B3

Strain data

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