avatar

Elena Velázquez

Last updated: Sep 01, 2021

P. putida KT2440 - B3

P. putida KT2440 (pSEVA421-Cas9tr // pSEVA6511-GIIi(B3)-94a) was barcoded with barcode #3 (TGGACATACATAGTATACTCTGGTG corresponds to the universal region present in all barcodes). Ll.LtrB intron delivery was used to carry out the insertion of the barcode and CRISPR/Cas9-mediated counterselection was implemented to facilitate the procedure.

Draft

Velázaquez E., et al. Targetron-assisted delivery of exogenous DNA sequences into Pseudomonas putida through CRISPR-aided counterselection (in preparation)

#
Strain data
Barcode
v1 de28be

de28be3dbcdc481f900b14ba6160d1a6b2ccc42cc

v1 TGGACA

Genosignature: TGGACATACATAGTATACTCTGGTGTTGAAGTTTCAAGGTTTTATCCGTAGGTTCAACTGCGTTGGGAGTGGGCAAGGGGGAATGGGCATAGATATAAAAACAATGGGGTTAGTAGGTTATCCATAGACTTGACAAGTTTGCCTTTG

Show GC content diagram

Pseudomonas putida

ATCC 12633

EV138 (Elena's stock collection)

Elena Velázquez

Centro Nacional Biotecnología (CNB-CSIC), Madrid (Spain). Elena Velázquez Stock collection