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JonathanT

Last updated: Jul 16, 2020

pUT18CTraVN

TraVN  (N. gonorrhoeae MS11 NCBI:CP003909) was PCR amplified using primer pair 17/18 and cloned into pUT18C

Draft
Barcode
v1 9d2632

9d2632797b79409da79b432e69ec56edb2cc60bc6

Whole genome sequence

This plasmid was constructed in this work: Protein interactions within and between two F-type type IV secretion systems. Birgit Koch, Melanie M. Callaghan, Jonathan Tellechea Luzardo, Ami Y. Seeger, Joseph P. Dillard, and Natalio Krasnogor. 2020. Submitted to Molecular Microbiology. The backbone (pUT18C) comes from: Karimova, G., Ullmann, A., and Ladant, D. (2001) Protein-protein interaction between Bacillus stearothermophilus tyrosyl-tRNA synthetase subdomains revealed by a bacterial two-hybrid system. J Mol Microbiol Biotechnol 3: 73–82.

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Strain data

Plasmids

This plasmid was propagated using 10-beta strain: Δ(ara-leu) 7697 araD139 fhuA ΔlacX74 galK16 galE15 e14-  ϕ80dlacZΔM15  recA1 relA1 endA1 nupG  rpsL 

This plasmid was created in this work from pUT18C

This plasmid is  based on pUT18C (Karimova et al 2001): Bacterial two-hybrid vector designed to express a given polypeptide fused in frame at its N-terminal end with T18 fragment; ColE1 ori, AmpR.

Ampicillin resistance

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