avatar

JonathanT

Last updated: Jul 17, 2020

pUTM18CTraVN

TraVN  (N. gonorrhoeae MS11 NCBI:CP003909) was PCR amplified using primer pair 17/18 and cloned into pUTM18C

Draft
Barcode
v1 7d8e79

7d8e798d9d76413dabb2e6c4f86e391db2cf71ef7

Whole genome sequence

This plasmid was constructed in this work: Protein interactions within and between two F-type type IV secretion systems. Birgit Koch, Melanie M. Callaghan, Jonathan Tellechea Luzardo, Ami Y. Seeger, Joseph P. Dillard, and Natalio Krasnogor. 2020. Submitted to Molecular Microbiology. The backbone (pUTM18C) comes from: Ouellette, S.P., Gauliard, E., Antosová, Z., and Ladant, D. (2014) A Gateway ® -compatible bacterial adenylate cyclase-based two-hybrid system: A Gateway-compatible bacterial two-hybrid system. Environ Microbiol Rep 6: 259–267.

#
Strain data

Plasmids

This plasmid was propagated using 10-beta strain: Δ(ara-leu) 7697 araD139 fhuA ΔlacX74 galK16 galE15 e14-  ϕ80dlacZΔM15  recA1 relA1 endA1 nupG  rpsL 

This plasmid was created in this work from pUTM18C

This plasmid is based on pUTM18C (Ouellette et al., 2014): As pUT18C but designed to insert the TM domain 1 of E. coli oppB between the cloned polypeptide and the T18 fragment; ColE1 ori, AmpR.

Ampicillin resistance

Devonshire Building, Newcastle University. ICOS -80 room.