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JonathanT

Last updated: Jul 16, 2020

pUT18CTraVF

TraVF was PCR amplified using primer pair 59/60 and cloned into pUT18C

Draft
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v1 0fc099

0fc099e7a3e74515bcd2adecc3f766e9b2ce41fe4

Whole genome sequence

This plasmid was constructed in this work: Protein interactions within and between two F-type type IV secretion systems. Birgit Koch, Melanie M. Callaghan, Jonathan Tellechea Luzardo, Ami Y. Seeger, Joseph P. Dillard, and Natalio Krasnogor. 2020. Submitted to Molecular Microbiology. The backbone (pUT18C) comes from: Karimova, G., Ullmann, A., and Ladant, D. (2001) Protein-protein interaction between Bacillus stearothermophilus tyrosyl-tRNA synthetase subdomains revealed by a bacterial two-hybrid system. J Mol Microbiol Biotechnol 3: 73–82.

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Strain data

Plasmids

This plasmid was created in this work from pUT18C

This plasmid is based on pUT18C (Karimova et al 2001): Bacterial two-hybrid vector designed to express a given polypeptide fused in frame at its N-terminal end with T18 fragment; ColE1 ori, AmpR. 

Ampicillin resistance

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