Last updated: Jul 16, 2020


TraVF was PCR amplified using primer pair 59/60 and cloned into pUT18C

Whole genome sequence

This plasmid was constructed in this work: Protein interactions within and between two F-type type IV secretion systems. Birgit Koch, Melanie M. Callaghan, Jonathan Tellechea Luzardo, Ami Y. Seeger, Joseph P. Dillard, and Natalio Krasnogor. 2020. Submitted to Molecular Microbiology. The backbone (pUT18C) comes from: Karimova, G., Ullmann, A., and Ladant, D. (2001) Protein-protein interaction between Bacillus stearothermophilus tyrosyl-tRNA synthetase subdomains revealed by a bacterial two-hybrid system. J Mol Microbiol Biotechnol 3: 73–82.

Strain data
v1 0fc099



This plasmid was created in this work from pUT18C

This plasmid is based on pUT18C (Karimova et al 2001): Bacterial two-hybrid vector designed to express a given polypeptide fused in frame at its N-terminal end with T18 fragment; ColE1 ori, AmpR. 

Ampicillin resistance

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