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JonathanT

Last updated: Aug 04, 2021

BW25113 - wild type

This is the wild type strain used for barcoding purposes in E. coli

The script used to find the barcoding site (essential_pair_finder.py) is attached. The script uses as input the .gff3 annotation file (BW25113.gff3) and the essential genes file (keio_genes_synonimschanged.txt). 

It prints as output the closest pair of essential genes.

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fe7c1691ee1c4bcda1444aaa4a3bfaf9b2c357b35

Whole genome sequence

Datsenko, KA and Wanner, BL (2000) One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc. Natl. Acad. Sci. U.S.A. 97:6640-5

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Strain data

Escherichia coli

ATCC 11775

BW25113

The initial aliquot of BW25113 cells was obtained from our strain collection in Devonshire Building.

rrnB3 ΔlacZ4787 hsdR514 Δ(araBAD)567 Δ(rhaBAD)568 rph-1.

Newcastle University, Devonshire Building. ICOS -80 room.