BW25113 - wild type
This is the wild type strain used for barcoding purposes in E. coli.
The script used to find the barcoding site (essential_pair_finder.py) is attached. The script uses as input the .gff3 annotation file (BW25113.gff3) and the essential genes file (keio_genes_synonimschanged.txt).
It prints as output the closest pair of essential genes.
Datsenko, KA and Wanner, BL (2000) One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc. Natl. Acad. Sci. U.S.A. 97:6640-5
Escherichia coliATCC 11775
The initial aliquot of BW25113 cells was obtained from our strain collection in Devonshire Building.
rrnB3 ΔlacZ4787 hsdR514 Δ(araBAD)567 Δ(rhaBAD)568 rph-1.
Newcastle University, Devonshire Building. ICOS -80 room.