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JonathanT

Last updated: Aug 05, 2021

168 - BC659

This is the barcoded version of 168 using the toxin-mediated recombination as barcoding method (Lin et al, 2013). The DNA cassette was PCR amplified from the in-vitro assembly of all the parts.

Commited
Barcode
v1 dcabd7

dcabd7cccc09420682210d4384131e65b2c2242df

v1 CTTACT

CTTACTATGCCACATAGTCTAACTACTTTGCGTAGGTAATAGAGCAACGAGTAGGGTTATCGTCTTTCATACTCGGTTGGCGATTATACAAACTCCAATGGTTTGATTGGTGTTTCTCATACG

Whole genome sequence

This strain was produced in this study using the procedure described in: Z. Lin et al., “A versatile mini-mazF-cassette for marker-free targeted genetic modification in Bacillus subtilis,” J. Microbiol. Methods, vol. 95, pp. 207–214, 2013, doi: 10.1016/j.mimet.2013.07.020.

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Strain data

Bacillus subtilis

ATCC 6051

s361

from parent

trpC2, BC659 (in between rpsD and tyrS genes)

Trp(-)

Newcastle University, Devonshire Building. ICOS -80 room.

Nov 04, 2019