BY4742 - BC14
This is the barcoded version of BY4742 using CRISPR as barcoding method. The barcoding plasmid used was pSC-CRISPR-BC (attached).Commited
Barcoding protocol based on: M. M. Jessop-Fabre et al., “EasyClone-MarkerFree: A vector toolkit for marker-less integration of genes into Saccharomyces cerevisiae via CRISPR-Cas9,” Biotechnol. J, vol. 11, pp. 1110–1117, 2016, doi: 10.1002/biot.201600147.
Saccharomyces cerevisiaeDSMZ DSM-70449
S288C-derivative laboratory strain: MATα his3Δ1 leu2Δ0 lys2Δ0 ura3Δ0. BC14 (inserted between NCA3 and ASF1)
Auxotrophic for: histidine, leucine, lysine and uracil.
Newcastle University, Devonshire Building. ICOS -80 room.
May 09, 2019